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Simultaneous analysis of vitamin D and K in processed food products via ultra high- performance liquid chromatography (UHPLC)

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Abstract

This study highlights a simplified extraction and method development of Ultra High-Performance Liquid Chromatography for simultaneous determination of vitamin D and K in selected processed food products. Simultaneous analysis of vitamin D and K was achieved via gradient elution of methanol with ultra-pure water (95:5–100:0–95:5), at programmed flow rate of (1.0–0.2–1.0 mL/min) for left pump and constant 1 mL/min for right pump within 25 min on HPLC Dionex Ultimate 3000 Series system using two Dionex Acclaim Polar Advantage II C18 columns at 30 °C and acquisition wavelength of 265 nm. The limits of detection and quantification ranged between 0.05 and 0.12 µg/mL and 0.16–0.41 µg/mL; for both vitamins. The method showed good linearity (R2: 0.996–0.999) and high accuracy (85.35–106.36%) for both vitamin D and K. Recovery above 70% was achieved for vitamin D, whereas lower recovery of 60–70% was obtained for vitamin K. The proposed analytical and extraction procedure was advantageous for sample purification and improved detection of trace vitamin levels from selected cereal and flour products.

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References

  1. J.D. Floros, R. Newsome, W. Fisher, G.V. Barbosa-Cánovas, H. Chen, C.P. Dunne, J.B. German, R.L. Hall, D.R. Heldman, M.V. Karwe, S.J. Knabel, T.P. Labuza, D.B. Lund, M. Newell-McGloughlin, J.L. Robinson, J.G. Sebranek, R.L. Shewfelt, W.F. Tracy, C.M. Weaver, G.R. Ziegler, Feeding the world today and tomorrow: the importance of food science and technology. Compr. Rev. Food Sci. Food Saf. 9, 572–599 (2010). https://doi.org/10.1111/j.1541-4337.2010.00127.x

    Article  Google Scholar 

  2. C.M. Weaver, J. Dwyer, V.L. Fulgoni, J.C. King, G.A. Leveille, R.S. MacDonald, J. Ordovas, D. Schnakenberg, Processed foods: contributions to nutrition. Am. J. Clin. Nutr. 99, 1525–1542 (2014). https://doi.org/10.3945/ajcn.114.089284

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. J.S. Sidhu, Y. Kabir, F.G. Huffman, Functional foods from cereal grains. Int. J. Food Prop. 10, 231–244 (2007). https://doi.org/10.1080/10942910601045289

    Article  CAS  Google Scholar 

  4. B. McKevith, Nutritional aspects of cereals. Nutr. Bull. 29, 111–142 (2004). https://doi.org/10.1111/j.1467-3010.2004.00418.x

    Article  Google Scholar 

  5. G. Montenegro-Bethancourt, M. Vossenaar, L.D. Kuijper, C.M. Doak, N.W. Solomons, Ready-to-eat cereals are key sources of selected micronutrients among schoolchildren from public and private elementary schools in Quetzaltenango, Guatemala. Nutr. Res. 29, 335–342 (2009). https://doi.org/10.1016/j.nutres.2009.05.003

    Article  CAS  PubMed  Google Scholar 

  6. P. Kumar, R. Yadava, B. Gollen, S. Kumar, R. Verma, S. Yadav (2011) Nutritional contents and medicinal properties of wheat: A review. Life Sci. Med. Res. 22, 1–10

    Google Scholar 

  7. D.-N. Rathi, C.Y. Liew, M.N.M. Fairulnizal, D. Isameyah, G. Barknowitz, Fat-soluble vitamin and carotenoid analysis in cooking oils by ultra-performance convergence chromatography. Food Anal. Methods 10, 1087–1096 (2017). https://doi.org/10.1007/s12161-016-0661-9

    Article  Google Scholar 

  8. T. Plozza, V.C. Trenerry, D. Caridi, The simultaneous determination of vitamins A, E and β-carotene in bovine milk by high performance liquid chromatography–ion trap mass spectrometry (HPLC–MSn). Food Chem. 134, 559–563 (2012)

    Article  CAS  Google Scholar 

  9. J.L. Luque-García, M.D. Luque de Castro, Extraction of fat-soluble vitamins. J. Chromatogr. A 935, 3–11 (2001). https://doi.org/10.1016/S0021-9673(01)01118-9

    Article  PubMed  Google Scholar 

  10. S. Scalia, A. Renda, G. Ruberto, F. Bonina, E. Menegatti, Assay of vitamin A palmitate and vitamin E acetate in cosmetic creams and lotions by supercritical fluid extraction and HPLC. J. Pharm. Biomed. Anal. 13, 273–277 (1995)

    Article  CAS  Google Scholar 

  11. A. Jedlicka, J. Klimes, Determination of water-and fat-soluble vitamins in different matrices using High-performance liquid chromatography. Chem. Pap. 59, 202–222 (2005)

    CAS  Google Scholar 

  12. A. Kamal-Eldi, S. Gorgen, J. Pettersson, A.M. Lampi, Normal-phase high-performance liquid chromatography of tocopherols and tocotrienols. Comparison of different chromatographic columns. J. Chromatogr. A 881, 217–227 (2000)

    Article  CAS  Google Scholar 

  13. P. Salo-Väänänen, V. Ollilainen, P. Mattila, K. Lehikoinen, E. Salmela-Mölsä, V. Piironen, Simultaneous HPLC analysis of fat-soluble vitamins in selected animal products after small-scale extraction. Food Chem. 71, 535–543 (2000). https://doi.org/10.1016/S0308-8146(00)00155-2

    Article  Google Scholar 

  14. C.F. Klopfenstein, Nutritional quality of cereal-based foods, in Handbook of cereal science and technology, revised and expanded. Food science and technology, vol. 99, 2nd edn., ed. by K. Kulp, J.G. Ponte (CRC Press, Marcel Dekker, New York, 2000), pp. 705–724

    Google Scholar 

  15. L. Cordain (1999) Cereal grains: humanity’s double-edged sword. in: Simopoulos A (ed) Evolutionary Aspects of Nutrition and Health: Diet, Exercise, Genetics and Chronic disease, vol 84. World Rev Nutr Diet, 1999/09/18 edn. Karger Medical and Scientific Publisher, USA, pp 19–73

    Chapter  Google Scholar 

  16. L. Henderson, K. Irving, J. Gregory (2003) The National Diet and Nutrition Survey: adults aged 19 to 64 years. Vitamin and Mineral Intake and Urinary Analytes. 3

  17. H.C.O.T. Netherlands (2012) Evaluation of the dietary reference values for vitamin D

  18. C. Vermeer, K. HamulyÁK, Vitamin K: lessons from the past. J. Thromb. Haemost. 2, 2115–2117 (2004). https://doi.org/10.1111/j.1538-7836.2004.01002.x

    Article  CAS  PubMed  Google Scholar 

  19. M.J. Shearer, C. Bolton-Smith, The UK food data-base for vitamin K and why we need it. Food Chem. 68, 213–218 (2000). https://doi.org/10.1016/S0308-8146(99)00157-0

    Article  CAS  Google Scholar 

  20. T.N.C.O. Ministers (2014) Nordic Nutrition Recommendations 20125th edn. Integrating nutrition and physical activity

  21. P.O.M.I.O. Medicine (2001) Dietary reference intakes for Vitamin A, Vitamin K, Arsenic, Boron, Chromium, Copper, Iodine, Iron, Manganese, Molybdenum, Nickel, Silicon, Vanadium, and Zinc. J. Acad. Nutr. Diet. 101(3), 294 (2001)

  22. N.C. Binkley, D.C. Krueger, T.N. Kawahara, J.A. Engelke, R.J. Chappell, J.W. Suttie, A high phylloquinone intake is required to achieve maximal osteocalcin gamma-carboxylation. Am. J. Clin. Nutr. 76, 1055–1060 (2002)

    Article  CAS  Google Scholar 

  23. TurKomp (2014) Turkish Food Composition Database. http://www.turkomp.gov.tr/food/129,143,151,376. Accessed 29 June 2017

  24. USDA (2016) USDA National Nutrient Database for Standard Reference Release 28. http://www.ndb.nal.usda.gov/ndb/foods/show/1788,1849,2055,4794,5981,6545. Accessed 9th August 2017

  25. R. Macrae, R.K. Robinson, M.J. Sadler, Cereals, in Encyclopaedia of food science, food technology and nutrition, 1st edn., ed. by M.J. Sadler (Academic Press Inc., San Diego, London, 1993), pp. 768–789

    Google Scholar 

  26. C. MacEvilly, Cereals/contribution to the diet, in Encyclopedia of Food Sciences and Nutrition, ed. by L. Trugo, P. Finglass, B. Caballero (Academic Press, San Diego, 2003), pp. 1008–1014

    Chapter  Google Scholar 

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Acknowledgements

The authors wish to thank the Director General of Health Malaysia and the Director of Institute for Medical Research (IMR) for granting the permission to publish this study. We would also like to express our gratitude to the staff of Nutrition Unit for their valuable support. This project was funded by a grant from the Ministry of Health Research Grant.

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  1. Nutrition Unit, Cardiovascular, Diabetes and Nutrition Research Centre, Institute for Medical Research, Ministry of Health, Jalan Pahang, 50588, Kuala Lumpur, Malaysia

    Devi-Nair Rathi, Mohd Fairulnizal Md Noh, Aswir Abd Rashed & Isameyah Dasuki

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  1. Devi-Nair Rathi
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  2. Mohd Fairulnizal Md Noh
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Rathi, DN., Md Noh, M.F., Abd Rashed, A. et al. Simultaneous analysis of vitamin D and K in processed food products via ultra high- performance liquid chromatography (UHPLC). Food Measure 13, 1947–1957 (2019). https://doi.org/10.1007/s11694-019-00113-8

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