Characterization and effect of extraction solvents on the yield and total phenolic content from Vernonia amygdalina leaves

  • Oluwaseun Ruth Alara
  • Nour Hamid Abdurahman
  • Siti Kholijah Abdul Mudalip
  • Olusegun Abayomi Olalere
Original Paper


In this study, the effects of extraction solvents on the yield and total phenolic content of Vernonia amygdalina leaf were examined. The considered solvents were water, 20% v/v ethanol, 40% v/v ethanol, 60% v/v ethanol, 80% v/v ethanol, and 100% v/v ethanol using Soxhlet extraction technique. Highest extraction yield (19.45 ± 0.22% g/g) and total phenolic content (96.25 ± 2.52 mg GAE/g d.w.) were achieved using 60% v/v aqueous ethanol solution. In addition, there exist a significant correlation between total phenolic content and antioxidant activities using DPPH (R2 = 0.9397, p < 0.05) and ABTS scavenging activity (R2 = 0.7700, p < 0.05). More so, the FTIR analysis confirmed the presence of functional groups attributed to its antioxidant property. There was a significant damage in the cell wall of V. amygdalina leaf after extracted with 60% v/v ethanol solution. Therefore, V. amygdalina leaf can be a good source of antioxidants.


Vernonia amygdalina Solvent Antioxidant Extraction Phenolic Characterization 



The authors wish to acknowledge the financial support of Universiti Malaysia Pahang through the soft grant RDU160325.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest on this study.


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Copyright information

© Springer Science+Business Media, LLC 2017

Authors and Affiliations

  1. 1.Faculty of Chemical Engineering & Natural ResourcesUniversiti Malaysia PahangGambangMalaysia

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