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Characterizing the memory of the GAL regulatory network in Saccharomyces cerevisiae

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Systems and Synthetic Biology

Abstract

Genetic regulatory networks respond dynamically to perturbations in the intracellular and extracellular environments of an organism. The GAL system in the yeast Saccharomyces cerevisiae has evolved to utilize galactose as an alternative carbon and energy source, in the absence of glucose in the environment. We present a dynamic model for GAL system in Saccharomyces cerevisiae, which includes a novel mechanism for Gal3p activation upon induction with galactose. The modification enables the model to simulate the experimental observation that in absence of galactose, oversynthesis of Gal3p can also induce the GAL system. We then characterize the memory of the GAL system as the domain of attraction of the steady states.

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Acknowledgments

This research is supported, in parts, by NSF CAREER Award 0845650 and by Department of Science and Technology India.

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Authors and Affiliations

  1. Department of Electrical Engineering, Indian Institute of Technology, Bombay, Mumbai, 400 076, India

    Vishwesh V. Kulkarni

  2. Department of Chemical Engineering, Indian Institute of Technology, Bombay, Mumbai, 400 076, India

    Venkatesh Kareenhalli & Ganesh A. Viswananthan

  3. Department of Electrical and Computer Engineering, University of Minnesota, Minneapolis, MN, 55455, USA

    Marc Riedel

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  1. Vishwesh V. Kulkarni
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  2. Venkatesh Kareenhalli
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  3. Ganesh A. Viswananthan
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  4. Marc Riedel
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Correspondence to Vishwesh V. Kulkarni.

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Kulkarni, V.V., Kareenhalli, V., Viswananthan, G.A. et al. Characterizing the memory of the GAL regulatory network in Saccharomyces cerevisiae . Syst Synth Biol 5, 97–104 (2011). https://doi.org/10.1007/s11693-011-9086-3

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  • DOI: https://doi.org/10.1007/s11693-011-9086-3

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