Skip to main content
SpringerLink
Log in

Alleviation of cell damage in experimental ANP in rats by administration of chondroitin-sulfate reduces

  • Research Article
  • Published:
Frontiers of Medicine in China Aims and scope Submit manuscript

Abstract

In order to explore the effects of retrograde infusion of chondroitin-sulfate via the pancreatic duct on cytoprotection and attenuation of oxidative damage during acute necrotic pancreatitis (ANP), male Wistar rats were randomly divided into three groups: A, B (experimental groups) and C (sham operation, control group). The rats in group A was subjected to retrograde injection of 5% sodium taurocholate via the pancreatic duct, and those in group B received chondroitin-sulfate therapy after ANP induction. All rats in three groups were killed at 6 h. The levels of malondialdehyde (MAD), total superoxide dismutase (SOD), glutathione (GSH), adenosine triphosphate (ATP) and serum amylase (SAM) were measured. The morphologic changes in pancreatic tissues were observed. It was found that the level of SAM was increased in group A and group B, with corresponding pathological changes of ANP. The levels of ATP, GSH and SOD in group A were decreased markedly and MDN increased significantly as compared with those in group B (P<0.01). In group B, the histopathologic damage was attenuated to a certain extent in comparison to that in group A. It was concluded that endogenous antioxidants were significantly reduced and lipid peroxidation increased during ANP. Retrograde infusion of chondroitin-sulfate via pancreatic duct could alleviate the pancreatic cell damage as a sort of scavengers of oxygen free radicals.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Aho H J, Suonpaa K, Aho la R A, Nevalainen T J. Experimental pancreatitis in the rat-ductal factors in sodium taurocholate-induced acute pancreatitis. Exp Path, 1984, 25(2): 73–79

    Article  CAS  Google Scholar 

  2. Hansson K, Lundh G, Stenram U. The toxic effects of bile salts on the pancreas. Acta Chir Scand, 1967, 375(1): 48–64

    Google Scholar 

  3. Aho H J, Koskensalo S M L, Nevalainen T J. Experimental pancreatitis in the rat Sodium taurocholate-induced acute haemorrhagic pancreatitis. Scand J Gastroenterol, 1980, 15(3): 411–416

    Article  CAS  PubMed  Google Scholar 

  4. Gough D B, Boyle B, Joyce W P, Delaney C P, McGeeney K F, Gorey T F, Fitzpatrick J M. Free radical inhibition and serial chemiluminescence in evolving experimental pancreatitis. Br J Surg, 1990, 77(11): 1258–1259

    Article  Google Scholar 

  5. Formela C S, Galloway S W, Kingsnorth A N. Inflammatory mediators in acute pancreatitis. Br J Surg, 1995, 82(1): 6–13

    Article  CAS  PubMed  Google Scholar 

  6. Sakorafas G H, Tsiotou A G. Etiology and pathogenesis of acute pancreatitis: current concepts. J Clin Gastroenterol, 2000, 30(2): 343–356

    Article  CAS  PubMed  Google Scholar 

  7. Sweiry J H, Mann G E. Role of oxidative stress in the pathogenesis of acute pancreatitis. Scand J Gastroenterol Suppl, 1996, 219(1): 10–15

    Article  CAS  PubMed  Google Scholar 

  8. Albertini R, Deluca G, Passi A, Moratti R, Abuja P M. Chondroitin-4-Sulfate protects high-density lipoprotein against copper-dependent oxidation. Arch Biochem Biophys, 1999, 365(1): 143–149

    Article  CAS  PubMed  Google Scholar 

  9. Albertini R, Passi A, Abuja P M, De Luca G. The effect of glycos-aminoglycans and proteoglycans on lipid peroxidation. Int J Mol Med, 2000, 6(2): 129–136

    CAS  PubMed  Google Scholar 

  10. Campo G M, Avonoso A, Campo S, Ferlazzo A M, Altavilla D, Micali C, Calatroni A. Aromatic trap analysis of free radicals production in experimental collagen-induced arthritis in the rat: protective effect of glycosaminoglycans treatment. Free Radic Res, 2003, 37(3): 257–268

    Article  CAS  PubMed  Google Scholar 

  11. Campo G M, Avonoso A, Campo S, Ferlazzo A M, Altavilla D, Calatroni A. Efficacy of treatment with glycosaminglycans on experimental collagen-induced arthritis in rats. Arthritis Res Ther, 2003, 5(3): 122–131

    Article  Google Scholar 

  12. Niederau C, Ferrell L D, Grendell J H. Caerulein-induced acute necrotizing pancreatitis in mice: protective effects of proglumide, benzotript, and secretin. Gastroenterology, 1985, 88(9): 1192–1204

    CAS  PubMed  Google Scholar 

  13. Ellman G L. Tissue sulphydryl groups. Arch Biochem Biophys, 1959, 82(1): 70–77

    Article  CAS  PubMed  Google Scholar 

  14. Salzman A L, Menconi M J, Unno N, Ezzell R M, Casey D M, Gonzalez P K, Fink M P. Nitric oxide dilates tight junctions and depletes ATP in cultured Caco-2Bbe intestinal epithelial monolayers. Am J Physiol, 1995, 268(2 Pt 1): G261–G373

    Google Scholar 

  15. Schulz H U, Niedrau C, Klonowski-Stumpe H, Halangk W, Luthen R, Lippert H. Oxidative stress in acute pancreatitis. Hepatogastroenterology. 1999, 46(29): 2736–2750

    CAS  PubMed  Google Scholar 

  16. Grune T, Reinheckel T, Davies K J. Degradation of oxidized proteins in mammalian cells. FASEB J, 1997, 11(5): 526–534

    CAS  PubMed  Google Scholar 

  17. Niederau C, Ude K, Niederau M, Strohmeyer G, Ferrell L D, Grendell J H. Effects of the seleno-organic substance Ebselen in two different models of acute pancreatitis. Pancreas, 1991, 6(3): 282–290

    Article  CAS  PubMed  Google Scholar 

  18. Wang Z, Iguchi H, Ohshio G. Increased pancreatic metallothionein and GSH levels protecting against cerulean-and taurocholate-induced acute pancreatitis in rats.Pancreas, 1996, 13(2): 173–183

    Article  CAS  PubMed  Google Scholar 

  19. Niederau C, Niederau M, Borchard F, Ferrell L D, Grendell J H. Effects of antioxidants and free radical scavengers in three different models of acute pancreatitis.Pancreas, 1992, 7(5): 486–496.

    Article  CAS  PubMed  Google Scholar 

  20. Sugahara K, Kitagawa H. Recent advances in the study of the biosynthesis and function of sulfated glycosaminoglycans. Curr Opin Strct Biol, 2000, 10(5): 518–527

    Article  CAS  Google Scholar 

  21. Beren J, Hill SL, Diener-West M, Rose NR. Effect of pre-loading oral glucosamine HCL/Chondroition Sulfate/manganese ascorbate combination on experimental arthritis in rats. Exp Biol Med, 2001, 226(2): 144–151

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of General Surgery, the First Affiliated Hospital, China Medical University, Shenyang, 110001, China

    He Zhongye PhD, Guo Renxuan PhD & Li Yang PhD

  2. Department of Pathology, College of Basic Medical Sciences, China Medical University, Shenyang, 110001, China

    Xie Chengyao MD & Liu Nan MD

  3. Department of Pathology, 242nd Hospital of Shenyang, Shenyang, 110001, China

    Song Wen MD

Authors
  1. He Zhongye PhD
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Guo Renxuan PhD
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Xie Chengyao MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Liu Nan MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Li Yang PhD
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Song Wen MD
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Guo Renxuan PhD.

Additional information

Translated from J Chin Med Univ, 2006, 35(3): 297–298, 301 [译自: 中国医科大学学报]

Rights and permissions

Reprints and permissions

About this article

Cite this article

He, Z., Guo, R., Xie, C. et al. Alleviation of cell damage in experimental ANP in rats by administration of chondroitin-sulfate reduces. Front. Med. China 1, 36–40 (2007). https://doi.org/10.1007/s11684-007-0007-5

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11684-007-0007-5

Keywords

Search

Navigation