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Lokale Applikation von Deferroxamin zur Verbesserung der Regeneration peripherer Nerven im Tierversuch

Influence of local deferoxamine application on peripheral nerve regeneration in an animal study

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Zusammenfassung

Noch immer stellt die Verbesserung der Ergebnisse nach Verletzung peripherer Nerven eine Herausforderung für die Wissenschaft dar. Ziel dieser Arbeit war es, den Einfluss eines Eisenchelators, nämlich des Deferroxamins (DFO), auf die Regeration peripherer Nerven zu untersuchen.

Bei Tieren der Gruppe I (n=12) wurde der rechte N. medianus nach dessen Durchtrennung mit einer einfachen End-zu-End-Nervennaht behandelt. Die Nahtseite wurde in Gruppe II (n=12) zusätzlich mit einem 1 cm langen V.-jugularis-externa-Segment umhüllt. Bei Tieren der Gruppe III (n=12) wurde die Vene mit unbeladenen Lipidpartikeln und in Gruppe IV (n=12) mit DFO beladenen Partikeln (Perineurin©) gefüllt. Zur Analyse der Regeneration des peripheren Nerven evaluierten wir postoperativ Greiftest, Histologie, Elektrophysiologie und das Muskelgewicht.

Der Vergleich der Ergebnisse aller Gruppen zeigte eine erhöhte Nervenfaserdichte sowie eine schnellere funktionelle Nervenregeneration im Greiftest bei Tieren der Gruppe IV. Die Anwendung von DFO führte bei der Neurographie zu einer herabgesetzten Reizschwelle und Latenz sowie einer erhöhten Nervenleitgeschwindigkeit.

So belegen die Ergebnisse dieser Studie einen positiven Einfluss der lokalen Anwendung von DFO auf die Regeneration am peripheren Nerven nach einer einfachen Nervennaht.

Abstract

Improving results after peripheral nerve injury is still a challenge for science. The aim of the present study was to investigate the effect of local deferoxamine (DFO) administration on postoperative outcome in the rat median nerve reconstruction model.

In group I (n=12) animals, the right median nerve was repaired by end-to-end neurorhaphy after dissection. The suture site was additionally wrapped by a 1 cm empty external jugular vein segment in group II (n=12). In animals of group III (n=12), the vein was filled with unloaded lipid particles, and in group IV animals (n=12) the vein was filled with DFO-loaded lipid particles (Perineurin©) For assessment of peripheral nerve regeneration, we postoperatively evaluated the grasping test, histology, electrophysiology, and muscle weight.

Comparing the results of all groups, we could show that intraoperative application of DFO-loaded lipid particles at the neurorhaphy site led to a significant increase in the density of regenerating axons as well as to an accelerated recovery of both muscle tropism and motor function. The electrophysiological results demonstrated a threshold decrease, lower latency, and higher conduction velocity in the DFO-treated animals.

The results of the present study suggest that local administration of DFO might have therapeutic potential for improving postoperative outcome after microsurgical nerve reconstruction.

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Authors and Affiliations

  1. Klinik für Hand-, Plastische-, Rekonstruktive- und Verbrennungschirurgie, Universität Tübingen, BG-Unfallklinik, Schnarrenberg 95, 72076, Tübingen, Deutschland

    F. Werdin, P. Schönle, A. Kraus, P. Jaminet, H.-E. Schaller & N. Sinis

  2. Dipartimento di Scienze Cliniche e Biologiche, University de Torino, Ospedale San Luigi, Orbassano, Italy

    Federica Di Scipio & S. Geuna

  3. Neuraxo Biopharmaceuticals, Düsseldorf, Erkrath, Deutschland

    G. Koopmanns

  4. Natruwissenschaftliches und Medizinisches Institut, Reutlingen, Deutschland

    T. Danker & E. Guenther

  5. Department of Hand and Plastic Surgery, Orthopädische Klinik Markgröningen, Markgröningen, Deutschland

    M. Haerle

  6. Neurologische Klinik der Universität Düsseldorf, Labor für Molekulare Neurobiologie, Düsseldorf, Deutschland

    H.-W. Mueller

Authors
  1. F. Werdin
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  2. Federica Di Scipio
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  3. P. Schönle
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  4. A. Kraus
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  7. T. Danker
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  8. E. Guenther
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  9. M. Haerle
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  10. H.-E. Schaller
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  11. S. Geuna
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  12. H.-W. Mueller
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  13. N. Sinis
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Correspondence to F. Werdin.

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Werdin, F., Di Scipio, F., Schönle, P. et al. Lokale Applikation von Deferroxamin zur Verbesserung der Regeneration peripherer Nerven im Tierversuch. Obere Extremität 4, 217–223 (2009). https://doi.org/10.1007/s11678-009-0044-6

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  • DOI: https://doi.org/10.1007/s11678-009-0044-6

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