Abstract
In our previous screening of the transcriptome of the causal agent of the devastating pine wilt disease, pine wood nematode (PWN, Bursaphelenchus xylophilus), after treatment with the nematicide fomepizole, Surfeit locus gene sft-4, which encodes a regulatory factor, was found to be downregulated. In situ hybridization results showed that the sft-4 was continuously expressed from egg to adult and was especially high in the reproductive system. Here in a study of the effect of RNA interference (RNAi) of sft-4 and recombinant SFT-4 on PWN activity, treatment with sft-4 dsRNA inhibited feeding, reproduction, oviposition and egg hatching of PWN with the greatest inhibition on reproduction and oviposition, whereas recombinant SFT-4 had the opposite effect. In addition, RNAi of sft-4 changed the female–male ratio and lifespan of PWN. In bioassays of PWNs, with RNAi of sft-4 on seedlings and 2-year-old Pinus thunbergii trees, none of the treated plants developed symptoms during the monitoring period, indicating that virulence of PWNs was either significantly weakened. These results indicate that the influence of sft-4 on PWN pathogenicity may be mainly through regulating reproductive function of PWN and its lifespan.
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Introduction
Pine wilt disease (PWD) caused by pine wood nematode (PWN, Bursaphelenchus xylophilus) causes serious ecological damage and economic losses worldwide (Takai et al. 2003; Akiba et al. 2012; Futai 2013; Li et al. 2021; Feng et al. 2022; Meng et al. 2022). At present, PWD management mainly relies on eliminating PWN or its insect vectors. Injecting nematicides against PWN into a tree trunk is considered one of the most effective and sustainable strategies to control PWD because it is less deleterious than spraying pesticides (Takai et al. 2003; Barbosa et al. 2012; Shanmugam et al. 2018; Liu et al. 2019; Lee et al. 2020). However, commonly used nematicides have single-action targets and their frequent use leads to resistance in the nematodes (Shanmugam et al. 2018; Liu et al. 2019). Hence, novel drug targets are greatly needed for developing promising nematicides against PWN.
The virulence of PWN is closely related to the spread of PWD (Wang et al. 2019). So far, many genes have been shown to be involved in the virulence of PWN. For example, cytochrome P450 gene has been shown to play a key role in the mechanism of low temperature tolerance in PWNs and be involved in regulating growth, development and longevity of nematodes (Wang et al. 2020). The pectate lyase gene is essential for successful invasion of their host plants by plant-parasitic nematodes. PWNs penetrate plant tissues by secreting pectate lyase, promoting feeding and migration in pine trees, eventually leading to host cell death and pine wilt. When pectate lyase gene expression was inhibited using RNAi, the reproduction and mobility of the PWN was inhibited and ultimately its pathogenicity or virulence was reduced in pine seedlings (Qiu et al. 2016). Inhibition of arginine kinase expression also decreases fertility and increases mortality of PWNs. These genes are thus potential targets for new nematicidal drugs against PWN.
We previously found that fomepizole, an inhibitor of alcohol dehydrogenase (ADH), rapidly killed PWNs (Wang 2019; Wang et al. 2019). By analyzing the transcriptome data of fomepizole-treated PWNs, we identified the differentially expressed gene Surfeit locus (sft-4) (Wang 2019). The sft family is widely present in the genome of animals and plays various important roles in organisms. Mashkevich et al. (1997) found that a homolog of mammalian surf-1 in yeast encodes a mitochondrial membrane protein related to respiration. Mouse surf-3 encodes ribosomal protein L7a (Giallongo et al. 1989). Homologs of sft-4 are widespread in invertebrate genomes and encode an integral membrane protein associated with the endoplasmic reticulum (ER) (Armes and Fried 1996). However, reports of the function of sft genes in PWN are rare. In the present research, we explored the physiological effect of manipulations of sft-4 on PWN to determine whether the gene is a potential drug target for PWN control.
Materials and methods
Experimental materials
PWNs were isolated from wilted black pines in Yantai, China using the Baermann funnel technique and cultured on Botrytis cinerea grown on potato dextrose agar (PDA) in the dark at 25 °C (Booth 1971; Viglierchio and Schmitt 1983; Guo et al. 2017).
Cloning of sft-4 and construction of expression vectors
Total RNA was extracted from the mixed-stage PWNs using Trizol reagent as reported by Chen et al. (2011) and used to synthesize cDNA with a reverse transcription kit (Takara, Dalian, China). The gene sft-4 was amplified by polymerase chain reaction (PCR) using primers designed for the sft-4 sequence (forward: 5′-GTTACGGTCATCGTCCAGGTCATAC-3′; reverse: 5′-CTTCTCTTCCAAGGTCTCGGCATAC-3′). The amplified products were ligated into pET-15b using T4-DNA ligase (Takara, Dalian, China) (Wang et al. 2022).
Bioinformatics analysis of sft-4 and SFT-4
The open reading frame (ORF) of sft-4 from PWN was analyzed using the ORF Finder program (NCBI, Bethesda, MD, USA; http://www.ncbi.nlm.nih.gov/gorf/gorf.html). The structure of PWN sft-4 was analyzed using WormBase ParaSite (Howe et al. 2016, 2017). Signal peptides in SFT-4 were predicted using the server SignalP 5.0 (http://www.cbs.dtu.dk/services/SignalP-5.0/), and transmembrane helices were predicted using the server TMHMM 2 (http://www.cbs.dtu.dk/services/TMHMM/).
Fluorescence in situ hybridization to localize sft4 expression in PWNs at different stages
A sulfo-cyanine3-labeled probe (5′-Cy3-CGGAGACCGATTTCAACAGCCCGTCGACTGTCGACTTGAGCTTGG-3′) was designed based on the ORF sequence of PWN sft-4 using Premier 5.0 software (PREMIER Biosoft International, San Francisco, CA, USA). The mixed-stage nematodes were fixed in 4% v/v RNase-free paraformaldehyde (PFA) aqueous solution at 5 °C for 16 h before using the WISH01 in situ hybridization kit (Gefan, Shanghai, China) and the manufacturer’s protocol. Samples were examined for fluorescence at the excitation wavelength of 554 nm and emission wavelength of 568 nm using a fluorescence microscope (Nikon Eclipse Ci, Tokyo, Japan).
Expression and purification of recombinant SFT-4
Escherichia coli BL21 (DE3) (Takara, Dalian, China) was transformed with the expression vector pET-15b-sft-4, then plated on LB medium to select bacteria that express recombinant SFT-4. A single colony was then cultured in LB fermentation broth, after 4 h, 0.5 mM isopropyl-beta-D-thiogalactopyranoside (IPTG) was added to the broth to induce expression of recombinant SFT-4 at 28 °C (Zhou et al. 2021). The bacteria cells were collected by centrifugation at 10,000 rpm for 20 min, then suspended in 20 mL binding buffer (20 mM Tris-HCl, 0.5 M NaCl, 5 mM imidazole, pH 8.0), and lysed using an ultrasonic processor (400 W, 4 s, 15 s, 120 cycles), followed by centrifugation at 10,000g for 25 min. The recombinant SFT-4 was purified using Ni-NTA affinity chromatography (Xu et al. 2015).
Effects of recombinant SFT-4 on PWN vitality
PWNs were soaked in a solution of recombinant SFT-4 in Tris-HCl buffer (pH 8.0) and tested for locomotion (Zhou et al. 2021), feeding (Wang et al. 2019, 2022), reproduction (Xu et al. 2015; Meng et al. 2019; Zhou et al. 2021), oviposition and hatching (Wang et al. 2019, 2022; Zhou et al. 2021) as described previously. PWNs soaked in Tris-HCl buffer (pH 8.0) and BSA solution were used as the two controls, respectively. The BSA control was set to exclude the side effect of the protein. To determine whether recombinant SFT-4 can enter the body of PWN, the nematodes were soaked in purified GFP under the same conditions, then washed with sterilized water three times, and observed at the excitation wavelength of 488 nm and emission wavelength of 507 nm with a fluorescence microscope (Olympus IX73, Tokyo, Japan).
Synthesis of sft-4 dsRNA and confirmation of RNAi
With pET-15b-sft-4 as the template, three DNA fragments, P1 (537 bp), P2 (441 bp), P3 (366 bp), selected from the ORF of sft-4 were PCR-amplified using T7-labeled gene-specific primers (F1: 5′-GATCACTAATACGACTCACTATAGGGCTC CTCCCGCATATCGCCCGACTTTG-3′; R1: 5′-GATCACTAATACGACTCACTAT AGGGGGTCTTATAGCCAATGGTGAC-3′; F2: 5′-GATCACTAATACGACTCACT ATAGGGTTTTTGGCCAGGGACATCTCAG-3′; R2: 5′-GATCACTAATACGACT CACTATAGGGGTCGGACTCCACCAG-3′; F3: 5′-GATCACTAATACGACTCACT ATAGGGTCTCAAGAAGTCACCGACAAC-3′; R3: 5′-GATCACTAATACGACTC ACTATAGGGCTTATAATGATAGTCGACTGAG-3′). A 323-bp DNA fragment of the GFP gene was amplified using a pair of T7-labeled gene-specific primers (forward: 5′-GATCACTAATACGACTCACTATAGGGAACGGCCA CAAGTTCAGC-3′; reverse: 5′-GATCACTAATACGACTCACTATAGGGAAGTCGATGCCCTTCAGC-3′) as the negative control. With the amplicons as templates, double-stranded RNA (dsRNA) for sft-4 was synthesized using the MEGAscript RNAi Kit (Invitrogen, Vilnius, Lithuania) and the manufacturer’s instructions. RNAi was carried out using the soak method previously described by Xu et al. (2015). Briefly, approximately 3000 PWNs were soaked in 50 µL of solution containing sft-4 dsRNA (1.0 µg/µL) at 20 °C for 72 h. PWNs were soaked in 50 µL sterilized water and GFP dsRNA solution (1.0 µg/µL) and used as double negative controls. The efficiency of RNAi was assessed by qRT-PCR using forward primer 5′-GGTCATCGTCCAGGTCA TACT-3′ and reverse primer 5′-TTCCAAGGTCTCGGCATACA-3′). The gene for actin from PWN was used as an internal control using forward primer 5′-CTGCTGAGCGTGAAATCGT-3′ and reverse primer 5′-GTTGTAGGTGGTCTCGTGGA-3′). The data were analyzed using the 2 − △△Ct method. There were three biological replicates in this experiment.
Effects of sft-4 dsRNA on PWN vitality and lifespan
The effects of sft-4 dsRNA on PWN motility, reproduction, oviposition and egg hatching were determined as described in Sect. 2.6. Approximately 100 RNAi-treated PWNs were added to each well of three 48-well plates to evaluate the lifespan of nematodes. The experiment was done three times.
Effect of RNAi on pathogenicity of PWN
Approximately 100 30-day-old Pinus thunbergii seedlings and 1000 of 2-year-old P. thunbergii (about 1000 pieces) saplings were inoculated with RNAi-treated PWNs (Yu et al. 2012; Xu et al. 2015; Wang et al. 2020). PWNs treated with sterilized water and GFP dsRNA were used as double negative controls. The virulence of PWNs was determined after 15 days according to the wilting severity on seedlings (Yu et al. 2012).
Data analyses
All experiments were carried out in triplicate and means ± standard deviation (SD) calculated. Means among treatments were compared for significant differences using Student’s t-tests in SPSS version 17.0 (SPSS, Chicago, IL, USA) and GraphPad (Boston, MA, USA) Prism 8. A significance level of P < 0.05 was applied.
Results
Cloning and sequencing of sft-4 coding gene in PWN
Sequence analysis of the sft-4 PCR product showed an 852-bp ORF encoding a protein composed of 283 amino acids with a relative molecular weight of 31 kDa (Fig. 1a). The amino acid sequence of SFT-4 was 46%, 49%, and 46% homologous to that of Aphelenchus avenae (GenBank accession KAH7713954.1), Pristionchus Pacificcus (GenBank accession KAF8382798.1) and Bursaphelenchus okanawaensis (GenBank accession CAD5229971.1), respectively (Fig. 1b). The theoretical pI of PWN SFT-4 is 7.00, and its amino acid sequence was predicted to have five transmembrane helical structures (Fig. 1c) and no signal peptide sequence (Fig. 1d).
Bioinformatic analysis of sft-4. a Nucleotide and deduced amino acid sequence of sft-4 from pine wood nematodes (PWNs). b Alignment of the amino acid sequences of SFT-4 from PWNs, Aphelenchus avenae, Pristionchus pacificus and Bursaphelenchus okinawaensis. Colors indicate conserved amino acid residues. Predicted c SFT-4 signal peptide and d SFT-4 transmembrane structure
Localization of sft-4 expression in PWN
Red hybridization signals for sft-4 were found throughout the life cycle of PWN. Red fluorescence was detected nearly throughout the eggs and J1 nematodes (Fig. 2a, b). Signals in J2–J4 nematodes were mainly concentrated in the intestinal tract and the tail (Fig. 2c–e). In male and female adults, the signal was strong in the genital area, especially in the spicules of males (Fig. 2f, g).
Localization of sft-4 mRNA in pine wilt nematodes (PWNs) using fluorescence in situ hybridization. Hybridization with red-fluorescence-labeled probe in a the egg b J1 juvenile, c J2 juvenile, d J3 juvenile, e J4 juvenile, f male adult, g female adult. Hybridization for negative controls in h egg, i J1 juvenile, j J2 juvenile, k male adult and l female adult. m, metacorpus; an, anus. Scale bar = 50 µm
Effect of recombinant SFT-4 on PWN
The relative molecular weight of the recombinant SFT-4 protein overexpressed in E. coli BL21 (DE3) after IPTG treatment was approximately 33 kDa as shown by SDS-PAGE analysis (Fig. 3), consistent with the predicted molecular size. The homogeneity of recombinant SFT-4 purified from the supernatant of the engineered bacterial lysate by Ni2+ affinity chromatography was verified by SDS-PAGE (Fig. 3).
SDS-PAGE analyses of purification and identification of recombinant SFT-4. a Isolated recombinant SFT-4. Lane 1: Total proteins of E. coli BL21 (DE3) harboring pET-15b-sft-4; after centrifugation at 10,000×g for 25 min, lanes 2, 3: supernatant; lane 4: pellet. b SDS-PAGE analysis of purified recombinant SFT-4. Lane 1: Total proteins of E. coli BL21 (DE3); Lane 2: total proteins of E. coli BL21 (DE3) harboring pET-15b-sft-4; Lane 3: purified recombinant protein
Green fluorescence was detected in the GFP-treated PWNs, which indicated that SFT-4 can infiltrate PWN bodies (Fig. 4). The motility of SFT-4-treated PWNs was not significantly different from that of the controls (Fig. 5a) (P > 0.05), while the feeding rate (Fig. 6), reproductive ability (Fig. 5b), oviposition rate (Fig. 5c) and hatching rate (Fig. 5d) were significantly higher than for the controls (P < 0.01). These results indicated that recombinant SFT-4 may play an important role in regulating feeding and reproduction of PWN.
Effect of recombinant SFT-4 on vitality of pine wilt nematodes (PWNs). a Mean number of head thrashes per 30 s, b the number of nematodes reproduced by the inoculated PWNs, and c egg hatching rate after treatment with recombinant SFT-4 (control treatments: Tris-HCl buffer, BSA). Means were tested for significant differences using Student’s t-test; bars on means are standard errors; level of significance: **P < 0.005, ***P < 0.001, ns, not significant
Effect of sft-4 dsRNA on PWN
The qRT-PCR to analyze the expression level of sft-4 after treatment with the three synthesized interfering fragments showed that fragment 2 inhibited expression the most (Fig. 7), so we selected fragment 2 for the subsequent sft-4 RNAi experiment. Compared with the relative expression level for sft-4 in the group treated with sterilized water, relative expression sft-4 in PWN treated with sft-4 dsRNA was strongly inhibited the expression (76.6% lower than in the control), and there was no significant effect in the GFP dsRNA-treated group (Fig. 7). Thus, treatments with sft-4 dsRNA inhibited expression of sft-4 in PWNs.
qRT-PCR results for relative expression of sft-4 in pine wilt nematodes (PWNs) after treatment with sft-4 dsRNA. RNAiP1, -P2, -P3 represent three synthesized RNA interfering fragments. Means were tested for significant differences using Student’s t-test; bars on means are standard errors; level of significance: *P < 0.05, ***P < 0.001, ns, not significant
Based on the PWN feeding assays, the feeding rate of PWNs after RNAi was much lower than for those treated with sterilized water or GFP dsRNA (Fig. 8). The moving rate of PWN after RNAi was also significantly lower than for the negative controls, with 30 head thrashes per 30 s for PWN treated with sft-4 dsRNA, 33 for sterilized water and GFP 32 dsRNA (Fig. 9a) (P < 0.01). The lifespan of PWNs after sft-4 dsRNA treatment was also significantly shorter than for those treated with sterilized water. The sft-4 dsRNA-treated PWNs reached 80% mortality in 12 days compared with 15 days for the control (Fig. 9b). In addition, the female-male ratio of PWNs in RNAi group was five times higher than the 1.3 ratio after the GFP dsRNA treatment and 1.5 after the sterilized water treatment (Fig. 9c). Moreover, the reproductive ability, oviposition rate and egg hatchability for sft-4 dsRN-treated PWNs were reduced significantly compared to that of the PWNs treated with sterilized water or GFP dsRNA (Fig. 9d). The PWNs treated with sft-4 dsRN produced 4273 eggs, compared with 15,885 and 17,288 eggs after the treatments with sterilized water and GFP dsRNA, respectively (Fig. 9e) (P < 0.01), and the hatchability of these eggs for the three treatment groups was 31.22%, 82.98% and 80.26%, respectively (Fig. 9f) (P < 0.01). These results indicated that sft-4 may play an important role in regulating PWN feeding, development, reproduction and lifespan.
Effects of treatment with sft-4 dsRNA on movement (a), lifespan (b), female–male ratio (c), reproduction (d), oviposition (e) and percentage of eggs that hatched (f) of pine wilt nematodes (PWNs). Control treatments: GFP dsRNA-treated and Sterilzed water. Means were tested for significant differences using Student’s t-test; bars on means are standard errors; level of significance: ***P < 0.001, ns, not significant
In the seedlings inoculated with PWN from the four treatment groups, the virulence of the PWNs treated with sft-4 dsRNA was significantly weaker than that of the control PWNs treated with sterilized water or GFP dsRNA. Specifically, the seedlings in the sterilized water group and GFP dsRNA group started wilting 7 days post inoculation (dpi), while the seedlings of RNAi group had no symptoms. On 15 dpi, the seedlings in both control groups had completely withered, whereas seedlings treated with sft-4 dsRNA still had no wilting (Fig. 10). The 2-year-old saplings in the sterilized water group and GFP dsRNA group had begun to wilt by 14 dpi, and most had completely wilted by 40 dpi. In contrast, the sft-4 dsRNA group never developed any symptoms. The results indicated that the sft-4 dsRNA treatment of the PWNs either abolished pathogenicity or significantly decreased their virulence (Fig. 11) by influencing feeding, reproduction and mobility of the PWNs.
Discussion and conclusions
In the present study, we studied the role of sft-4, which we previously determined was significantly downregulated in PWNs after treatment with fomepizole, which inhibits ADH and rapidly kills PWNs (Wang et al. 2019). The gene sft-4 and its homologues are widespread among organisms and play important regulatory roles in material transport and protein synthesis. In Caenorhabditis elegans, sft-4 is crucial for the endoplasmic reticulum (ER) transport of vitellin VIT-2, which is one of the core proteins of vitellin and often used to detect the transportation of yolk proteins in C. elegans (Grant and Hirsh 1999; Belden and Barlowe 2001; Balklava et al. 2007; Saegusa et al. 2018). Surf4, a homologue of sft-4 in mammalian cells, is involved in formation of the microsomal membrane and encodes a membrane protein (Reeves and Fried 1995). Several studies have indicated that surf4 is involved in the movement of materials by the ER and Golgi (Belden and Barlowe 2001; Mitrovic et al. 2008; Saegusa et al. 2018). Based on our studies, sft-4 overexpression in PWNs significantly increased the reproductive ability and oviposition, but RNAi of the gene significantly decreased reproduction and oviposition in PWNs with either weakened virulence or a loss of pathogenicity. In addition, our in situ hybridization analysis showed that sft-4 was more strongly expressed in the reproductive system of adults. We also verified that sft-4 affects PWN lifespan. Thus, sft-4 is important for the functioning of the PWN reproductive system. Hence, we speculate that the sft-4 might influence virulence or pathogenicity by regulating the reproduction and lifespan of the nematodes.
Overall, sft-4 affected PWN vitality, reproduction, oviposition, female to male ratio and lifespan and significantly influenced PWN virulence. Therefore, sft-4 is a potential drug target for developing a novel nematicide to control PWN.
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Conceptualization, SL, LW, RL, and GD; methodology, SL, LW, RL, CW, GD and QG; Software, SL; validation, RL, GD and QG; statistical analyses, SL; investigation, SL, LW, MC, WD; resources, RL, CW, GD and QG; data curation, SL, RL, GD and QG; writing original draft, SL; manuscript review and editing, RL, WD, GD and QG; visualization, SL, LW, MC; supervision, RL, GD and QG; project administration, RL, GD and QG; funding acquisition, RL and QG.
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This study was supported by the Shandong Provincial Natural Science Foundation, China (ZR2020MC123) and Qingdao Municipal People-benefitting Demonstration Project of Science and Technology, China (23-2-8-cspz-8-nsh).
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Liu, S., Wang, L., Li, R. et al. Cloning of sft-4 and its influence on vitality and virulence of pine wood nematode, Bursaphelenchus xylophilus. J. For. Res. 35, 43 (2024). https://doi.org/10.1007/s11676-023-01687-2
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DOI: https://doi.org/10.1007/s11676-023-01687-2