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Effects of chemical sterilization of the culture media, porous membranes and luminosity on in vitro culture of Eucalyptus grandis × Eucalyptus urophylla

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A Correction to this article was published on 30 November 2020

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Abstract

Hybrid combinations of Eucalyptus have increased due to expansion of plantations into unconventional areas and to the search for higher quality timber. However, most of these species have difficulties surviving in vitro cultivation. Active chlorine and sealing systems are often used to reduce contamination and increase gas exchange. The aim of the present study is to evaluate the establishment, multiplication, elongation and adventitious rooting of E. grandis × E. urophylla. Two clones (C1 and C2) and four active chlorine concentrations (0.000%, 0.001%, 0.003%, and 0.005%) were tested in the establishment and multiplication phases. Three sealing forms (W/M, 1/M and 3/M) and the same four active chlorine concentrations were applied to the elongation phase. Two luminosities (dark and light) and three sealings (W/M, 1/M and 3/M) were tested during adventitious rooting. Active chlorine concentration of 0.005% led to the lowest fungal contamination rate and to the highest in vitro establishment. Active chlorine concentration of 0.003% resulted in the greatest length and highest number of shoots per explant in the multiplication phase. There were no phytotoxicity problems and the quality of plants grown in an environment with active chlorine was maintained in comparison with those grown in an autoclave. The increase in gas exchange in ventilation systems had a positive impact on the in vitro growth and development of plants.

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Authors and Affiliations

  1. Department of Forestry Sciences, Federal University of Lavras, Lavras, Minas Gerais, Brazil

    Letícia Vaz Molinari, Denys Matheus Santana Costa Souza, Maria Lopes Martins Avelar, Sérgio Bruno Fernandes, Douglas Santos Gonçalves, Júlio Cézar Tannure Faria, Dulcineia de Carvalho & Gilvano Ebling Brondani

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  1. Letícia Vaz Molinari
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  2. Denys Matheus Santana Costa Souza
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  3. Maria Lopes Martins Avelar
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  4. Sérgio Bruno Fernandes
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  5. Douglas Santos Gonçalves
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  6. Júlio Cézar Tannure Faria
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  7. Dulcineia de Carvalho
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  8. Gilvano Ebling Brondani
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Correspondence to Letícia Vaz Molinari or Gilvano Ebling Brondani.

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The original online version of this article was revised: “In the original publication of the article, there were errors in the fig 3 and fig 6. The corrected figures are given below:”

Project funding: This work was carried out with the support of the Coordination for the Improvement of Higher Education Personnel - Brazil (CAPES), the National Council for Scientific and Technological Development (CNPq) and the Research Support Foundation of the State of Minas (FAPEMIG).

The online version is available at http://www.springerlink.com.

Corresponding editor: Yanbo Hu.

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Molinari, L.V., Souza, D.M.S.C., Avelar, M.L.M. et al. Effects of chemical sterilization of the culture media, porous membranes and luminosity on in vitro culture of Eucalyptus grandis × Eucalyptus urophylla. J. For. Res. 32, 1587–1598 (2021). https://doi.org/10.1007/s11676-020-01240-5

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  • DOI: https://doi.org/10.1007/s11676-020-01240-5

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