Linking tree growth rate, damage repair, and susceptibility to a genus-specific pest infestation
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Pest preference and subsequent susceptibility of a host individual is likely related to previous growth patterns in that host. Emerald ash borer (Agrilus planipennis Fairmaire) is a pestiferous beetle introduced to North America from Asia. While all species of ash are susceptible to attack, some individual trees appear to survive infestation. We selected ash trees in southeastern Michigan, collected cores and categorized trees as high tolerance to emerald ash borer attack (high overall health, low crown dieback), low tolerance (low overall health, high crown dieback) and intermediate tolerance (in-between the other categories). We artificially wounded trees and measured wound closure after 3 years. Ring width indices were not correlated between high and low tolerance trees. Regression slopes comparing growth and years were significantly different between the three tolerance categories, with high tolerance trees having the steepest slope. Wound closure was greatest in high tolerance trees. High tolerance trees demonstrating more rapid (steeper regression slope), consistent (lower variance), and effective (greater wound closure) growth. Those vigorously growing trees likely had more capacity to repair damage caused by emerald ash borer, leading to healthier trees in our categorization. Linking previous host growth patterns to health may have implications related to identifying individual trees potentially tolerant to attack.
KeywordsAgrilus Ash Dendrochronology Emerald ash borer Fraxinus Invasive species
Plant suitability for a pest insect is likely to align with the stress or vigor level of an individual host. However, there are two competing hypotheses as to which host type is more suitable: a stressed or vigorous individual host (Price 1991; White 1984). While these hypotheses originated based on specific pest-host interactions (stress hypothesis: psyllids (White 1984); vigor hypothesis: galling and shoot borers (Price 1991)), support exists for both, which may be system and interaction dependent. The plant vigor hypothesis suggests that pest insects are more successful and preferentially feed on vigorously growing plants (Price 1991; Inbar et al. 2001). The plant stress hypothesis suggests the opposite with stressed plants being more suitable (White 1969, 1984). These may be extremes and an intermediate stress hypothesis may be appropriate in other plant–insect interactions, especially where pulses of stress and recovery are more beneficial to insects (Huberty and Denno 2004).
First detected in 2002, emerald ash borer (Agrilus planipennis Fairmaire) was probably introduced to North America via wood packing material imported from Asia (Poland and McCullough 2006). Cross-dating analysis of cores from the affected area suggests that emerald ash borer went undetected for nearly 10 years and was introduced to southeastern Michigan, USA, and Ontario, Canada, in the early- to mid-1990s (Siegert et al. 2014). Since introduction, emerald ash borer has spread across much of the eastern half of the USA and Canada (Herms and McCullough 2014). All North American ash species (Fraxinus spp.) are susceptible to attack, including relatively healthy individuals (Liu et al. 2003; Cappaert et al. 2005; Anulewicz et al. 2006; Rebek et al. 2008).
The decline of ash and subsequent mortality is caused by the feeding of emerald ash borer larvae on vascular cambium and phloem layers of the tree (Bauer et al. 2004). Even in large, healthy trees, mortality can occur in a few years (Poland and McCullough 2006). Estimates regarding ash loss and potential retention in North American forests are broad, but agree that millions of trees have been lost to emerald ash borer and millions more are still at high risk of infestation (Cappaert et al. 2005; Poland and McCullough 2006; Marshall et al. 2013). Such loss has the potential for large-scale economic impacts, especially in urban forests where ash are removed and replaced (Vannatta et al. 2012). Mortality rates are related to the relative density of emerald ash borer larvae within trees, with high tree loss rates occurring in forest stands that are heavily infested (Marshall et al. 2009). Ash phloem connecting the crown to the roots becomes severed as emerald ash borer larvae feed and progress through developmental instars. Such severing needs to be repaired in order for the tree to survive. Previous growth rates and wound closure abilities may have an influence on the likelihood that an individual tree will succumb to, or survive damage (Shortle et al. 2003).
While the vast majority of ash do succumb to attack by emerald ash borer, there are some individual trees that survive and exhibit some level of resiliency (Marshall et al. 2013). Cipollini et al. (2011) demonstrated that there are clear differences between the phloem chemistries of North American ash species and those found in Manchurian ash. Since Manchurian ash co-evolved with emerald ash borer, there may be factors other than phloem chemistry leading to individual North American ash survival. The objectives of this study were to (1) quantify the growth rates of ash trees along a gradient of tolerance to emerald ash borer attack; (2) identify wound closure within the tolerance gradient; and (3) test the hypothesis that growth rates and wound closure influence presence of emerald ash borer attack signs.
Materials and methods
Study areas and trees
Tree core sampling
From each selected tree, two cores were collected at breast height and perpendicular to each other during early-July 2013 using a 4.3 mm diameter increment borer (Haglöf Sweden AB, Långsele, Sweden). Cores were air dried, mounted on wood rails for support, progressively sanded with 220–500 grit sandpaper, and measured to the nearest 0.001 mm using a Velmex UniSlide with linear encoder and a stereo microscope. Mean ring widths for each tree were converted to ring area (annual basal area growth, mm2), which represents overall tree growth better than ring width (Biondi and Qeadan 2008). COFECHA software (version 6.06P) was used to verify cross-dating among series and to identify possible measurement problems. Since all trees were alive when samples were collected with living phloem and had produced early wood during 2013, all series were aligned starting at 2012. While ring-porous trees, such as ash (Fraxinus spp.), rarely produce false or missing rings (Phipps 1985), there was still a need to ensure ring measurements were correlated with the created master chronology.
Analysis of variance (ANOVA) was used to compare DBH between tolerance groups. Mood’s median test was used to test if the count of trees with earliest measurable year were independent of tolerance groups. Ring width indices based on mean ring widths in each tolerance group were calculated using the spline method in R 3.5.1 software (R Core Team 2018) with the dplR library and Pearson’s correlation was used to compare ring width indices between tolerance groups. Simple linear regression was used to identify relationships between year and mean annual basal area growth for each tolerance group. Estimated marginal means of tolerance group regression models were calculated using emtrends function in emmeans package in R and slopes were compared with the pairs function in R as pairwise t tests with a Holm (1979) p value adjustment. Repeated measures ANOVA was used to compare tolerance group annual basal area growth variance with post hoc pairwise t tests and a Holm (1979) p value adjustment. Annual growth variance was calculated as mean for each of the previous year growth subtracted from the current year growth. Wound closure was compared between tolerance groups using ANOVA with a Tukey’s HSD post hoc test. We used logistic regression to test the relationships between wound closure and the presence of signs of emerald ash borer infestation (bark splits, exit holes, and wood pecker activity).
Thirty-five trees were categorized as high tolerance (Kensington = 14, Lower Huron = 2, Oakwoods = 19, Willow = 0), 31 as intermediate tolerance (Kensington = 21, Lower Huron = 2, Oakwoods = 7, Willow = 1), and 14 as low tolerance (Kensington = 7, Lower Huron = 0, Oakwoods = 6, Willow = 1). DBH did not significantly differ between trees within the three tolerance groups and had a pooled mean of (17.6 ± 5.7) cm (F2,72 = 1.43, p = 0.245). Seven ring series were flagged in the output from COFECHA due to relatively low correlations with the master chronology (r < 0.50; high tolerance = 4; intermediate tolerance = 2; low tolerance = 1). These were also the trees with the shortest ring series (10–14 years). Due to this lack of correlation, these trees were omitted from subsequent analyses.
Since its introduction to North America, emerald ash borer has caused significant mortality in ash species (Poland and McCullough 2006; Kovacs et al. 2010; Marshall et al. 2013). One important characteristic of the pest has been the success of emerald ash borer in a broad range of health categories in ash, with both healthy and stressed trees succumbing to attack (Herms and McCullough 2014). However, a small number of trees appear to have survived exposure to emerald ash borer, even though these trees have signs of attack (Hietala 2013; Marshall et al. 2013). Those trees that have remained provide an important source for data in testing if the plant vigor or plant stress hypothesis aided in explaining ash survival in the presence of emerald ash borer. Our objectives focused on understanding variation in growth rates, wound closure, and attack by emerald ash borer. We applied the plant stress hypothesis in an effort to explain differences in the presence of emerald ash borer attack signs in light of slower, variable growth rates and reduce wound closure rates.
We were able to separate trees using tolerance groups described by Hietala (2013), which were defined by vigor rating, percent crown dieback, and signs of emerald ash borer attack. Trees categorized into these tolerance groups were of similar size and we were able to collect cores that were measurable back to similar years in each tolerance group. While trees were not different in DBH, their growth rates and patterns differed, as evidenced by both ring width indices and annual basal area growth analyses. High tolerance trees had a steeper slope (faster growth rate) than both intermediate and low tolerance trees. Additionally, the low tolerance trees had significantly greater variance across years (less consistent growth) than both intermediate and high tolerance trees.
Faster, consistent growth rates leading to attack resistance or resilience aligns with studies for other beetle taxa. Hard (1985) identified patterns in white spruce (Picea glauca [Moench] Voss) resistance and resilience under attack by spruce beetle (Dendroctonus rufipennis [Kirby]), where unattacked trees had the greatest radial growth, killed trees had the least radial growth, and unsuccessfully attacked trees were in between. Similarly, the threshold for a successful attack by Ips typographus L. in Norway spruce (Picea abies [L.] Karst.) increased with increasing vigor (a ratio of basal area incremental growth and sapwood area; Mulock and Christiansen 1986). Radial growth in white oaks (Quercus alba L.) prior to infestation was significantly lower in trees subsequently colonized by twolined chestnut borer (Agrilus bilineatus [Weber]), a congener of emerald ash borer (Dunn et al. 1990). These examples support our interpretation that tree growth rates may provide an indication of resilience against pest attack; those trees with faster, more consistent growth rates have the highest likelihood of survival.
Price (1991) supported the plant vigor hypothesis, arguing that herbivorous pests benefit from vigorous host plants. This was posed as an alternative to the plant stress hypothesis where pests are more successful feeding on stressed plants (e.g. White 1969). Our results align more with the plant stress hypothesis, in which high tolerance trees exhibit higher growth rate and greater wound closure capacity compared to the low tolerance trees. Since the stress and vigor hypotheses provide differing explanations as to how pest insects interact with their host plant, especially those with a limited host range, conservative interpretation and application should be employed to explain an observed interaction. However, they still provide a framework for testing and explaining likely reasons for why a pest herbivore may select individual host plants. Both Price (1991) and Inbar et al. (2001) found support for the plant vigor hypothesis using endophytic insects. However, our endophytic insect (emerald ash borer) seems to align more with the plant stress hypothesis.
By categorical definition, the high tolerance trees had highest vigor (rating 1 or 2) compared to the low tolerance trees (rating 3–5). As wound closure values increased, the likelihood of emerald ash borer exit holes decreased. Vigorously growing trees with the ability to close the artificial phloem wounds likely were able to close the wounds produced by emerald ash borer as they fed in the phloem. Twolined chestnut borer has reduced success in oaks that have the ability to rapidly grow callus tissue and reconnect phloem (Dunn et al. 1990). Wound closure is a fairly precise measure of overall tree vigor; with suitable resource availability, trees can produce callus tissue and reconnect phloem (Neely 1970). By extension, the reduced likelihood of emerald ash borer exit holes as wound closure increased may be related to the ability of those vigorously growing trees to produce callus tissue as larvae feed. For other boring beetles, tree vigor and wound closure aligns with resilience to attack, with those trees closing wounds having reduced infestation rates (e.g. Dunn et al. 1990; Katovich et al. 2000; Fierke and Stephen 2008). Along with our results, those reported by Dunn et al. (1990) and summarized by Katovich et al. (2000) suggest that phloem feeding borers (i.e. Agrilus spp.) may preferentially select stressed hosts, likely due to callus tissue growth limitations.
It is important to keep in mind that all trees included in this study were green ash, which accounted for over 90% of the surviving ash within these forests (Marshall et al. 2013). While white ash (F. americana L.) did occur in the forest, it was much rarer in numbers. Adult capture rates have not differed between green and white ash in trapping studies (Marshall et al. 2009, 2010), which suggests an indifference in host selection in natural forests.
Because the high tolerance trees have maintained vigorous and consistent growth, they persist within the forest and may limit emerald ash borer emergence. The intermediate tolerance trees, while consistent in growth, were growing slower than the high tolerance trees and may lead to lower overall individual health. Finally, low tolerance trees were both slower and inconsistent in growth, making them the most susceptible to attack by emerald ash borer. There are high costs associated with removal and replacement of trees by urban foresters (Kovacs et al. 2010). By linking previous tree growth patterns to host health, these results have implications in identifying resilient individuals prior to heavy infestation by emerald ash borer (Clark et al. 2015). Mature trees with rapid, consistent growth should be retained within forests to ensure survival of ash within the tree community.
The authors thank Kelley Boyes, Ryan Boyes, Rachel Harman, Maja Sljivar, and Cecelia Smith for their assistance in field and laboratory data collection. Additionally, the authors thank the Huron-Clinton Metroparks for their continuing cooperation.
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