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Extraction optimization of insecticidal compounds from Lysurus mokusin by response surface methodology

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Abstract

We optimized the extraction process of Lysurus mokusin (L.) Fr. insecticidal compound (LMIC) using response surface methodology with ultrasonic extraction (UE). The surface morphologies of maceration extract (ME) and UE residues were compared by scanning electron microscopy, and then the activity of LMIC on Lymantria dispar (Asian gypsy moths) larvae was determined. Results showed that the optimal condition of UE was followed by 80 min of extraction time, 60% of ethanol concentration and 80 mL g−1 of liquid–solid ratio. The surface morphologies of UE residue were looser and rougher than ME residue. The corrected mortalities of LMIC on L. dispar larvae was 59.77%. This result indicated that L. mokusin was a good candidate as pesticide for pest management.

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Acknowledgements

My deepest gratitude goes to Professor Zou Li and Liu Xuefeng, their critical comments, constant encouragement and guidance have greatly enlightened me not only on the academic pursuit but also on the morals of being a man.

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Correspondence to Guocai Zhang.

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Project funding: The work was supported by the Central University Fundamental Research Funds Special Fund Project [2572016AA03, 25720171118], Harbin Applied Technology Research and Development Project [2016RAXXJ035] and the Central Financial Forest Science and Technology Promotion Demonstration Fund Project [JLT[2016]13].

The online version is available at http://www.springerlink.com

Corresponding editor: Tao Xu.

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Lin, L., Yang, J., Zhang, G. et al. Extraction optimization of insecticidal compounds from Lysurus mokusin by response surface methodology. J. For. Res. 31, 1985–1993 (2020). https://doi.org/10.1007/s11676-019-00880-6

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  • DOI: https://doi.org/10.1007/s11676-019-00880-6

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