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Plantlet regeneration of adult Pinus massoniana Lamb. trees using explants collected in March and thidiazuron in culture medium

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Abstract

A protocol for micropropagation using nodal explants from mature Pinus massoniana trees has been developed. Time of explant collection is crucial for the initial success of aseptic culture. Explants collected in early March gave the highest percentage of explant survival (64.5%) and shoot-forming percentage (52.3%). Thidiazuron (TDZ) concentration significantly influenced shoot formation; 4 μM TDZ was optimum, with 4.8 shoots produced per explant with a mean length of 7.1 cm after 120 days of culture. Regenerated shoots rooted for 60 days in basic medium with 1 μM NAA were ready for growth in pots. This is the first report on plantlet regeneration in vitro from mature trees of P. massoniana that provides a reliable method for propagating selected elites.

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Acknowledgements

This study was funded by the Science Research and Technology Development from the Department of Science and Technology of Guangxi (14125008-2-17, 1598006-5-7), the Natural Science Foundation of China (31360178), the Key Program of Guangxi Forestry Bureau ([2015]7), and as an independent project by the Key Laboratory of Gaungxi Fine Timber Forest Resources Cultivation (13A-01-01).

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Authors and Affiliations

  1. Guangxi Key Laboratory of Superior Timber Trees Resource Cultivation, Guangxi Forestry Research Institute, Nanning, 530002, Guangxi, People’s Republic of China

    Yin Wang & Ruiling Yao

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  1. Yin Wang
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  2. Ruiling Yao
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Correspondence to Ruiling Yao.

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The authors declare that they have no conflict of interest.

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Project funding: This work funded by the Science Research and Technology Development from the Department of Science and Technology of Guangxi (14125008-2-17, 1598006-5-7), the Natural Science Foundation of China (31360178), the Key Program of Guangxi Forestry Bureau ([2015]7), and as an independent project by the Key Laboratory of Gaungxi Fine Timber Forest Resources Cultivation (13A-01-01).

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Corresponding editor: Chai Ruihai.

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Wang, Y., Yao, R. Plantlet regeneration of adult Pinus massoniana Lamb. trees using explants collected in March and thidiazuron in culture medium. J. For. Res. 28, 1169–1175 (2017). https://doi.org/10.1007/s11676-017-0412-9

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  • DOI: https://doi.org/10.1007/s11676-017-0412-9

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