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Biochemical investigations during in vitro adventitious shoot regeneration in leaflet explants from nodal segments of a mature Albizia procera tree

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Abstract

The in vitro adventitious shoot differentiation in leaflet explants of an adult tree differed from that of leaflet explants of seedlings of Albizia procera (Roxb.) Benth. reported previously elsewhere. The leaflet explants from an adult tree passed through an initial callus phase for 30 days on MS medium supplemented with 3 % sucrose, 2.5 µM 2,4-D followed by a subsequent adventitious shoot differentiation phase for another 30 days on half MS medium supplemented with 0.25 µM each of BA and IBA. The regeneration rate of in vitro adventitious shoots in explants from the adult tree, i.e.1.66 shoots/callus, was lower than that from seedlings, i.e. >10 shoots/callus, which was reported elsewhere. Correspondingly, the activities of nitrate reductase and peroxidase, and endogenous phenol content remained very low during in vitro adventitious shoot differentiation in leaflet explants of an adult tree possibly due to lower availability of competent stem (juvenile) cells for the process.

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Abbreviations

2,4-D:

2,4-dichlorophenoxyacetic acid

BA:

Benzyladenine

IBA:

Indole-3-butyric acid

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Acknowledgments

We are grateful to the Director of Tropical Forest Research Institute Jabalpur for providing research facilities and Madhya Pradesh Council of Science and Technology, Bhopal for Project Grant (Sanction No. 3402/CST/BTAC/2009) during the course of the present investigation.

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Authors and Affiliations

  1. Genetics and Plant Propagation Division, Tropical Forest Research Institute, Jabalpur, 482021, India

    Ekta Rai, Sulochna Bouddha & Shamim Akhtar Ansari

  2. Institute of Forest Productivity, Lalgutwa, Ranchi, 835303, India

    Shamim Akhtar Ansari

Authors
  1. Ekta Rai
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  2. Sulochna Bouddha
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  3. Shamim Akhtar Ansari
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Corresponding author

Correspondence to Shamim Akhtar Ansari.

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Project funding: Madhya Pradesh Council of Science and Technology, Bhopal, India Project Grant Sanction No. 3402/CST/BTAC/2009.

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Corresponding editor: Zhu Hong

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Rai, E., Bouddha, S. & Ansari, S.A. Biochemical investigations during in vitro adventitious shoot regeneration in leaflet explants from nodal segments of a mature Albizia procera tree. J. For. Res. 27, 699–705 (2016). https://doi.org/10.1007/s11676-015-0196-8

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