Skip to main content
SpringerLink
Log in

Regulatory effect of salicylic acid and methyl jasmonate supplementation on ergosterol production in Hericium erinaceus mycelia

  • ORIGINAL PAPER
  • Published:
Journal of Forestry Research Aims and scope Submit manuscript

Abstract

We evaluated the biomass and ergosterol content of Hericium erinaceus mycelium, and extracellular enzyme activities in H. erinaceus liquid culture following salicylic acid (SA) and methyl jasmonic acid (MeJA) supplementation. The optimal SA concentration was 100 µmol·L−1, where the highest ergosterol content of 2.33 mg·g−1 was obtained following 6-day cultivation with 100 µmol·L−1 SA supplementation, and which was significantly higher than the unsupplemented control (p < 0.01). Following 4-day supplementation with 50 μmol·L−1 MeJA, the highest ergosterol content obtained was 1.988 mg·g−1, which was 25.8 % higher than the unsupplemented control. Our data indicate that SA and MeJA supplementation improves ergosterol content in H. erinaceus mycelium.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9
Fig. 10

Similar content being viewed by others

References

  • Blanco F, Salinas P, Cecchini NM, Jordana X, Van Hummelen P, Alvarez ME, Holuigue L (2009) Early genomic responses to salicylic acid in Arabidopsis. Plant Mol Biol 70:79–102

    Article  CAS  PubMed  Google Scholar 

  • Bulgakov VP, Tchernoded GK, Mischenko NP, Khodakovskays MV, Glazunov VP, Radchenko SV (2002) Effect of salicylic acid, methyl jasmonate, ethephon and cantharidin on anthraquinone production by Rubia cordifolia callus cultures transformed with the role B and role C gene. Biotechnol Lett 97(3):213–221

    CAS  Google Scholar 

  • Carvalhais LC, Dennis PG, Badri DV, Tyson GW, Vivanco JM, Schenk PM (2013) Activation of the jasmonic acid plant defence pathway alters the composition of rhizosphere bacterial communities. PLoS One 8:1–5

    Google Scholar 

  • Cheong JJ, Choi YD (2003) Methyl jasmonate as a vital substance in plants. Trends Genet 19:409–413

    Article  CAS  PubMed  Google Scholar 

  • Endo J, Takahashi W, Ikegami T, Beppu T, Tanaka O (2009) Induction of flowering by inducers of systemic acquired resistance in the Lemna plant. Biosci Biotechnol Biochem 73(1):183–185

    Article  CAS  PubMed  Google Scholar 

  • Goossens A, Hakkinen ST, Laakso I, Seppanen-Laakso T, Biondi S, De Sutter V, Lammertyn F, Nuutila AM, Soderlund H, Zabeau M, Inze D, Oksman-Caldentey KM (2003) A functional genomics approachtoward the understanding of secondary metabolism in plant cells. Proc Natl Acad Sci USA 100(14):8595–8600

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Halaouili S, Asther M, Sigoillot JC (2006) Fungal tryrosinases: new prospects in molecular characteristics. Bioengineering and biotechnological applications. Appl Microbiol 100:219–232

    Article  Google Scholar 

  • Han ZH, Zhang PQ, Kong XH, Ma QF, Dai XD, Zhang JC (2007) Extracellular enzyme activities, mycelial growth rates and fruit body yields of ten Auricularia auricular strains. Acta Edulis Fungi 14(4):41–46

    Google Scholar 

  • Han ZH, Zhang JC, Zhang PQ, Dai XD, Liu JN, Yu DS (2009) Study on the activity change of extracellular enzymes in stock culture of Auricularia Aauricular. Biotechnology 19(5):14–16

    CAS  Google Scholar 

  • Kang SM, Jung HY, Kang YM, Yunb DJ, Bahkb JD, Yang JK, Choi MS (2004) Effect of methyl jasmonate and salicylic acid on the production of tropane alkaloids and the expression of PMT and H6H in adventitious root cultures of Scopolia parviflora. Plant Sci 166(3):745–751

    Article  CAS  Google Scholar 

  • Kawagishi H, Shimada A, Shiral R, Okamoto K, Ojima F, Sakamoto H, Ishiguro Y, Furukawa S (1994) Erinacines A, B and C, strong stimuLators of nerve growth factors (NGF) synthesis from mycelia of Hericium erinaceum. Tetrahedron Lett 35:1569–1572

    Article  CAS  Google Scholar 

  • Kenmoku H, Sassa T, Kato N (2000) Isolation of erinacine P, a new parental metabolite of cyathane-xylosides, from Hericium erinaceum and its biomimetic conversion into erinacines A and B. Tetrahedron Lett 41:4389–4393

    Article  CAS  Google Scholar 

  • Khan A, Tania M, Liu R, Rahman MM (2013) Hericium erinaceus: an edible mushroom with medicinal values. J Complement Integr Med 10:253–258

    Article  Google Scholar 

  • Li J, Lu L, Dai C, Chen K, Qiu J (2001) A comparative study on sterols of ethanol extract and water extract from Hericium erinaceus. China J Chin Mater Med 26(12):831–834 (In Chinese)

    CAS  Google Scholar 

  • Ma BJ, Shen JW, Yu HY, Ruan Y, Wu TT, Zhao X (2010) Hericenones and erinacines: stimulators of nerve growth factor (NGF) biosynthesis in Hericium erinaceus. Mycology 1:92–98

    Article  CAS  Google Scholar 

  • Martin HM (2004) Induction of two indirect defences benefits Lima bean Phaseolus lunatus, Fabaceae in nature. J Ecol 92:527–536

    Article  Google Scholar 

  • Mattila P, Lampi A, Ronkainen R et al (2002) Sterol and vitamin D2 contents in wild and cultivated mushrooms. Food Chem 76:293–298

    Article  CAS  Google Scholar 

  • Miao Z, Wei Z, Yuan Y (2000) Study on the effects of salicylic acid on taxol biosynthesis. Chin J Biotechnol 16(4):509–513 (In Chinese)

    CAS  Google Scholar 

  • Mizuno T (1999) Bioactive substances in Hericium erinaceus (Bull.: Fr.) Pers. and its medicinal utilization. Int J Med Mushrooms 1:105–119

    Article  CAS  Google Scholar 

  • Qiu Y, Jia N, Wang L (2003) Progress of studies on elicitor’s application in taxal production in Taxus cell cyktyres. Chin Bull Bot 20(2):184–189 (In Chinese)

    Google Scholar 

  • Stintzi A, Weber H, Reymond P, Browse J, Farmer EE (2001) Plant defense in the absence of jasmonic acid: the role of cyclopentenones. PNAS 98:12837–12842

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Takaishi Y, Kawagishi Y (1991) Glycosides of ergosterol derivatives from Hericium erinaceus. Phytochemistry 30:4117–4120

    Article  CAS  Google Scholar 

  • Terashita T, Nakaie Y, Inoue T (1998) Role of metal proteinasesin the fruitbody formation of Hypsizygus marmoreus. J Wood Sci 44(5):379–384

    Article  CAS  Google Scholar 

  • Volkman JK (2003) Sterols in microorganisms. Appl Microbiol Biotechnol 60:495–506

    Article  CAS  PubMed  Google Scholar 

  • Wang ZJ, Luo DH, Liang ZY (2004) Structure of polysaccharides from the fruiting body of Hericium erinaceus pers. Carbohydr Polym 57:241–247

    Article  CAS  Google Scholar 

  • Wasternack C (2007) Jasmonates: an update on biosynthesis, signal transduction and action in plant stress response, growth and development. Ann Bot-london 100:681–697

    Article  CAS  Google Scholar 

  • Xiao CQ, Gao H, Chi R (2004) Progress in the application of elicitors to promote the production of plant secondary metabolites. Nat Prod Res Dev 16(5):473–476 (In Chinese)

    CAS  Google Scholar 

  • Xu W, Yan SC (2005) The function of jasmonic acid in induced plant defence. Acta Ecolog Sin 25(8):2074–2082 (In Chinese)

    CAS  Google Scholar 

  • Yang Y, Jiang R, Chen Y, Gao Q (2004) Study on chemical composition of Hericium erinaceus polysaccharide HEP-2. Nat Prod Res Dev 16:194–197

    CAS  Google Scholar 

  • Zhang A, Sun P, Zhang J, Tang C, Fan J, Shi X, Pan Y (2007) Structural investigation of a novel fucoglucogalactan isolated from the fruiting bodies of the fungus Hericium erinaceus. Food Chem 104:451–456

    Article  CAS  Google Scholar 

  • Zhang AQ, Fu L, Xu M, Sun PL, Zhang JS (2012) Structure of a water-soluble hetero-polysaccharide from fruiting bodies of Hericium erinaceus. Carbohydr Polym 88:558–561

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This research was supported by funding from the China Agriculture Research System (CARS-24) and the Heilongjiang Province Outstanding Youth Science Fund (JC201316).

Author information

Authors and Affiliations

  1. College of Life Science, Northeast Forestry University, Harbin, 150040, People’s Republic of China

    Xiaodong Dai & Yaguang Zhan

  2. Microbiology Institute of Heilongjiang Provincial Academy of Sciences, Harbin, 150010, People’s Republic of China

    Xiaodong Dai, Jiechi Zhang, Zenghua Han, Qingfang Ma, Xianghui Kong & Yinpeng Ma

  3. Institute of High Technology, Heilongjiang Academy of Science, Harbin, 150010, People’s Republic of China

    Xiaodong Dai, Piqi Zhang & Jianing Liu

Authors
  1. Xiaodong Dai
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yaguang Zhan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jiechi Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Piqi Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Zenghua Han
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Qingfang Ma
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Xianghui Kong
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Jianing Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Yinpeng Ma
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yaguang Zhan.

Additional information

Project funding: This research was supported by funding from the China Agriculture Research System (CARS-24) and the Heilongjiang Province Outstanding Youth Science Fund (JC201316)

The online version is available at http://www.springerlink.com

Corresponding editor: Zhu Hong

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Dai, X., Zhan, Y., Zhang, J. et al. Regulatory effect of salicylic acid and methyl jasmonate supplementation on ergosterol production in Hericium erinaceus mycelia. J. For. Res. 26, 71–77 (2015). https://doi.org/10.1007/s11676-014-0014-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11676-014-0014-8

Keywords

Search

Navigation