Abstract
The ultrastructural distribution and active location of ATPase and the ultrastructural variations were investigated in mesophyll cells of Cyclocarya paliurus seedlings after iso-osmotic salt/water treatments in combination with calcium regulation. C. paliurus seedlings were treated with five groups (control, 85 mM NaCl, 85 mM NaCl + 12 mM Ca(NO3)2, PEG iso-osmotic to 85 mM NaCl and PEG iso-osmotic to 85 mM NaCl +12 mM Ca(NO3)2) in a hydroponic system in a phytotron. Results show that under normal growth conditions, the ATPase activity was low and the enzyme was primarily located on the nucleus. After 12 days of iso-osmotic salt/water treatments, ATPase activity on the tonoplast increased. Osmiophilic globules for iso-osmotic water treatment were greater than that for iso-osmotic salt treatments. The ATPase activity increased and was mostly transferred onto the nucleus for calcium regulation treatment under iso-osmotic salt/water stresses, and the osmiophilic globules significantly decreased under iso-osmotic water stress with calcium regulation. The ATPase located on the nucleus indicated that the degree of salt/drought damage that seedlings suffered was slighter, while the amount of the enzyme located on the tonoplast showed that the degree of salt/drought damage there was more serious. After 4 and 20 days of isoosmotic treatments, the injury suffered by the leaf ultrastructures of C. paliurus seedlings for iso-osmotic treatment with calcium regulation was lower than those without calcium regulation, especially for the iso-osmotic water treatments. Preliminary analysis suggests that the injury suffered by C. paliurus seedlings was lower for iso-osmotic salt treatments than for iso-osmotic water treatments, while the effect of calcium regulation under iso-osmotic water stress was greater than that of the iso-osmotic salt stress.
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References
Ashraf M, Mcneilly T. 1987. Salinity effects on five cultivars/lines of pearl millet (Pennisetum americanum ILl Leeke). Plant and Soil, 103: 13–19.
Baxter I, Tchieu J, Sussman MR, Boutry M, Palmgren MG, Gribskov M, Haeper JF, Axelsen KB. 2003. Genomic comparison of Ptype ATPase ion pumps in Arabidopsis and rice. Plant Physiol, 132: 618–628.
Chen Jinhui, Shi Jishen, Gan Xihua. 2006. Ultra-cytochemical localization of ATPase activity in somatic embryogenesis of Liriodendron hybrid (Liriodendron chinese×L.tulipifera). Acta Botania Boreali-Occidentalia Sinica, 26(1): 12–17 (in Chinese).
Epimashko S, Fischer-Schliebs E, Christian AL, Thiel G, Lüttge U. 2006. Na+/H+-transporter, H+-pumps and an aquaporin in light and heavy tonoplast membranes from organic acid and NaCl accumulating vacuoles of the annual facultative CAM plant and halophyte Mesembryanthemum crystallinum L. Planta, 224: 944–951.
Fang Shengzuo, Fu Xiangxiang. 2007. Research progress and prospects on silviculture and utilization of Cyclocarya paliurus resources. Journal of Nanjing Forestry University, 31(1): 95–100. (in Chinese).
Fang S, Wang J, Wei Z, Zhu Z. 2006. Methods to break seed dormancy in Cyclocarya paliurus (Batal)Iljinskaja. Scientia Horticulturae, 110: 305–309.
Greenway H, Munns R. 1980. Mechanisms of salt tolerance in nonhalophytes. Plant Physiol, 31: 149–190.
Hasegawa PM, Bressnan RA, Zhu JK. 2000. Plant cellular and molecular responses to high salinity. Plant Physiol, 51: 463–499.
Hoagland DR, Arnon DI. 1950. The water culture method for growing plants without soil. Circ., California Agricultural Experimental Station, Berkeley, Calif, pp347.
Joshi PA, Stewart JM, Graham ET. 1988. Ultrastructural localization of ATPase activity in cotton fiber during elongation. Protoplasma, 143: 1–10.
Kalampanayil BD, Wimmers LE. 2001. Identification and characterization of a salt-stress-induced plasma membrane H+-ATPase in tomato. Plant Cell Environ, 24: 999–1005.
Kerkeb L, Donaire JP, Rodriguez-Rosales MP. 2001. Plasma membrane H+-ATPase activity is involved in adaptation of tomato calli to NaCl. Plant Physiol, 111: 483–490.
Krysan PJ, Young JC, Tax F, Sussman MR. 1996. Identification of transferred DNA insertions within Arabidopsis genome is involved in signal transduction and ion transport. Proc Natl Acad Sci USA, 93: 8145–8150.
Kuhlbrandt W. 2004. Biology, structure and mechanism of P-type ATPases. Nat Rev Mol Cell Biol, 5: 282–295.
Kurihara H, Fukami H, Kusumoto A, Toyoda Y, Shibata H, Matsui Y, Asami S, Tanaka T. 2003. Hypoglycemic action of Cyclocarya paliurus (Batal.) Iljinskaja in normal and diabetic mice. Biosci Biotechnol Biochem, 67(4): 877–880.
Lambers H. 2003. Dryland salinity: a key environmental issue in southern Australia. Plant and Soil, 257: 5–7.
Li Lei, Xie Mingyong, Yi Xing. 2002. Study on reducing blood sugar of polysaccharide from Cyclocarya paliurus. Chin Med, 25(1): 39–41. (in Chinese).
Martinez-Ballesta MC, Martinez V, Carvajal M. 2003. Aquaporin functionality in relation to H+-ATPase activity in root cells of Capsicum annuum grown under salinity. Plant Physiol, 117: 413–420.
Mi Haili, Zhen Guoqi, Xu Xing. 2006. Effects of NaCl stress on plasmalemma and tonoplast H+-ATPase activities in seedlings roots of Lycium barbarum in Ningxia Hui Autonomous region. Acta Botania Boreali-Occidentalia Sinica, 26(4): 748–752. (in Chinese).
Morsomme P, Boutry M. 2000. The plant plasma membrane H+-ATPase: structure, function and regulation. Biochim Biophys Acta, 1465: 1–16.
Peng FR, Guo J, Wang GP. 2004. Subcellular localization of vegetative storage protein of Ginkgo biloba. Acta Botanica Sinica, 46(1): 77–85.
Porillo F. 2000. Regulation of plasma membrane H+-ATPase in fungi and plants. Biochim Biophy Acta, 1469: 31–42.
Qiu QS, Guo Y, Quintero FJ. 2004. Regulation of vacuolar Na+/H+ pumping and plasma membrane H+- ATPase activity in proteoid roots of white lupin under phosphate deficiency. Plant Physiol, 279: 207–215.
Reinhold L, Seiden A, Volokita M. 1984. Is modulation of the rate of proton pumping a key event in osmoregulation. Plant Physiol, 75: 846–849.
Salem E, Micaela C, Rachid G. 2005. Study of the involvement of osmotic adjustment and H+-ATPase activity in the resistance of Catharanthus roseus suspension cells to salt stress. Plant Cell Tis Org Cul, 80: 287–294.
Sam O, Ramírez C, Coronado MJ. 2003. Changes in tomato leaves induced by NaCl stress: leaf organization and cell ultrastructure. Plant Biol, 47: 361–366.
Shi H, Lee BH, Wu SJ, Zhu JK. 2003. Overexpression of a plasma membrane H+-ATPase antiporter gene improves salt tolerance in Arabidopsis thaliana. Nat Biotechnol, 21: 81–85.
Sibole JV, Cabot C, Michalke W, Poschenrieder C, Barcelo J. 2005. Relationship between expression of the PM H+-ATPase, growth and ion partitioning in the leaves of salt-treated Medicago species. Planta, 221: 557–566.
Wang BS, Lüttge U, Rataj R. 2001. Effects of salt treatment and osmotic stress on V-ATPase and V-PPase in leaves of the halophyte Suaeda salsa. J Exp Bot, 52: 2355–2365.
Wang YZ, Sze H. 1985. Similarities and differences between the tonoplast-type and mitochondrial H+-ATPase of oat roots. J Biol Chem, 260: 10434–10443.
Xie Mingyong, Li Lei. 2001. Review in studies on chemical constituents and bioactivities of Cyclocarya paliurus. Chin. Tradition. Herb Drugs, 32(4): 365–366 (in Chinese).
Yang YL, Zhang F, Zhao MG, An LZ, Zhang LX, Chen NL. 2007. Properties of plasma membrane H+-ATPase in salt-treated Populus euphratica callus. Plant Cell Rep, 26: 229–235.
Yao Ruiling, Fang Shengzuo. 2007. Effect of NaCl stress and Calcium regulation on ion distribution in root tissue of Cyclocarya paliurus. Journal of Plant Resource and Environment, 16(2): 22–26 (in Chinese).
Yao Ruiling, Wang Yin, Fang Shengzuo. 2008. Cytochemical localization of the H+-ATPase and variation of ultra-structure in leaves of Cyclocarya paliurus seedlings under salt-stress. Plant physiology communication, 44(2): 206–210. (in Chinese).
Zhao KF, Fan H, Zhou S, Song J. 2003. Study on the salt and drought tolerance of Suaeda salsa and Kalanchoe claigremontiana under iso-osmotic salt and water stress. Plant Sci, 165: 837–844.
Zhao LQ, Zhang F, Guo JK, Yang YL, Li BB, Zhang LX. 2004. Nitric oxide functions as a signal in salt resistance in the calluses from two ecotypes of reed. Plant Physiol, 134: 849–857.
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Foundation project: This study was supported by the National Natural Science Foundation of China (Project No: 30371156) and Research Foundation of Jiangsu Province (Project No: BG2006314).
Biography: YAO Rui-ling (1979–), female, PhD, study interest in the theory and technology of forest afforestation, in College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, P. R. China.
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Yao, Rl., Fang, Sz., Shang, Xl. et al. Cytochemical localization of ATPase and sub-cellular variation in mesophyll cell of Cyclocarya paliurus seedlings under iso-osmotic stress and calcium regulation. Journal of Forestry Research 20, 343–348 (2009). https://doi.org/10.1007/s11676-009-0058-3
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DOI: https://doi.org/10.1007/s11676-009-0058-3