Abstract
Mangrove endophytic fungus 1893 was isolated from Kandelia candel from an estuarine mangrove on the South China Sea Coast. Two new lactones 1893A and 1893B, together with other known compounds, have been isolated from its fermentation broth. To classify the endophyte correctly for further industrial application, a combination of morphological and molecular techniques was used to approach its identity. The endophyte was compared with similar species having trichogynes or trichogyne-like hyphae which apparently fused with antheridium-like hyphae, and perithecia initials developing from an ascogonial coil surrounded by enveloping hyphae in early developmental stages on pure culture. Further morphological characteristics on host and non-host were used for comparison with similar species when the endophyte was cultivated on leaves of Kandelia candel and Mangifera indica, respectively, which resulted in classifying the endophyte as a Phomopsis species. The ITS sequence of rDNA was used to infer its phylogenetic relationships with Phomopsis species that resembled the strain in morphology or ecology. Finally, the endophyte was identified as Diaporthe phaseolorum var. sojae based on morphological and molecular evidence. Our study is a first report of Diaporthe phaseolorum var. sojae isolated from mangrove Kandelia candel.
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References
Castlebury LA, Farr DF, Rossman AY, Jaklitsch WJ. 2003. Diaporthe angelicae comb. nov., a modern description and placement of Diaporthopsis in Diaporthe. Mycoscience, 44: 203–208.
Castlebury LA, Rossman AY, Jaklitsch WJ, Vasilyeva LN. 2002. A preliminary overview of the Diaporthales based on large subunit nuclear ribosomal DNA sequences. Mycologia, 94(6): 1017–1031.
Chen GY, Lin YC, Vrijmoed LLP, Fong WF. 2006. A new isochroman from the marine endophytic fungus 1893#, Chem Nat Compds, 42:138–141.
Chen GY, Lin YC, Wen L, Vrijmoed LLP, Jones EBG. 2003. Two new metabolites of a marine endophytic fungus (No. 1893) from an estuarine mangrove on the South China Sea coast. Tetrahedron, 59: 4907–4909.
Chi PK, Jiang ZD, Xiang MM. 2007. Flora Fungorum Sinicorum Vol. 34 Phomopsis. Beijing: Science Press, 1–18. (in Chinese)
Farr DF, Castlebury LA, Pardo-Schultheiss RA. 1999. Phomopsis amygdali causes peach shoot blight of cultivated peach trees in the southeastern United States. Mycologia, 91: 1008–1015.
Farr DF, Castlebury LA, Rossman AY, Putnam ML. 2002. A new species of Phomopsis causing twig dieback of Vaccinium vitis-idaea (lingonberry). Mycol Res, 106(6): 745–752.
Fernández FA, Hanlin RT. 1996. Morphological and RAPD analysis of Diaporthe phaseolorum from Soybean. Mycologia, 88(3): 425–440.
Gilbert GS, Mejía-Chang M. 2002. Fungal diversity and plant disease in mangrove forests: salt excretion as a possible defense mechanism. Oecologia, 132:278–285.
Guo LD, Hyde KD, Liew ECY. 2000. Identification of endophytic fungi from Livistona chinensis based on morphology and rDNA sequences. New Phytol, 147(3): 617–630.
Huelsenbeck JP, Ronquist F. 2001. MRBAYES:Bayesian inference of phylogenetic trees. Bioinformatics, 17: 754–755.
Hobbs TW, Schmitthenner AF, Kuter GA. 1985. A new Phomopsis species from soybean. Mycologia, 77(4): 535–544.
Jensen JD. 1983. The development of Diaporthe phaseolorum variety sojae in culture. Mycologia, 75(6): 1074–1091.
Kanematsu S, Minaka N, Kobayashi T, Kudo A, Ohtsu Y. 2000. Molecular phylogenetic analysis of ribosomal DNA internal transcribed spacer regions and comparison of fertility in Phomopsis isolates from fruit trees. J Gen Plant Pathol, 66: 191–201.
Kathiresan K, Bingham BL. 2001. Biology of mangroves and mangrove ecosystems. Adv Mar Biol, 40:81–251.
Kulik MM. 1984. Symptomless infection, persistence, and production of pycnidia in host and no-host plants by Phomopsis batatae, Phomopsis phaseoli, and Phomopsis sojae, and the taxonomic implications. Mycologia, 76(2): 274–291.
Lin YC, Wu XY, Feng S, Jiang G, Luo JH, Zhou SN, Vrijmoed LLP, Jones EBG, Krohn K, Steingröver K, Zsila F. 2001. Five unique compounds: xyloketals from mangrove fungus Xylaria sp. from the South China Sea coast. J Org Chem, 66:52–62.
Luttrell ES, Huang LH. 1982. Development of the perithecium in Gnomonia comari (Diaporthaceae). Amer J Bot, 69(3): 421–431.
Maiello JM. 1978. The origin of the Pycnidium in Macrophomina phaseoli. Mycologia, 70(1): 176–179.
Mostert L, Crous PW, Kang JC, Phillips AJL. 2001. Species of Phomopsis and a Libertella sp. occurring on grapevines with specific reference to South Africa: morphological, cultural, molecular and pathological characterization. Mycologia, 93(1): 146–167.
Mucciarelli M, Scannerini S, Bertea CM, Maffei M. 2002. An ascomycetous endophyte isolated from Mentha piperita L.: biological features and molecular studies. Mycologia, 94(1): 28–39.
Murali TS, Suryanarayanan TS, Geeta R. 2006. Endophytic Phomopsis species: host range and implications for diversity estimate. Can J Microbiol, 52(7): 673–v680.
Nevena M, Jelena V, Franić-Mihajloviċ D. 1997. A comparative study of Diaporthe/Phomopsis fungi on soybean from two different regions of the world. Mycopathologia, 139: 107–113.
Posada D, Crandall KA. 1998. Modeltest: testing the model of DNA substitution. Bioinform, 14: 817–818.
Promputtha I, Lumyong S, Dhanasekaran V, Mckenzie EHC, Hyde KD, Jeewon R. 2007. A phylogenetic evaluation of whether endophytes become saprotrophs at host senescence. Micro Ecol, 53: 579–590.
Rehner SA, Uecker FA. 1994. Nuclear ribosome of internal transcribed spacer phylogeny and host diversity in the coelomuycete Phomopsis. Can J Bot, 72: 1666–1674.
Rossman AY, Farr DF, Castlebury LA. 2007. A review of the phylogeny and biology of the Diaporthales. Mycoscience, 48: 135–144.
Sanni MO. 1982. Perithecium development in Gelasinospora tetrasperma. Mycologia, 74(2): 320–324.
Schilder AMC, Erincik O, Castlebury L, Rossman A, Ellis MA. 2005. Characterization of Phomopsis spp. infecting grapevines in the great lakes region of north America. Plant Dis, 89: 755–762.
Selosse MA, Baudoin E, Vandenkoornhuyse P. 2004. Symbiotic microorganisms, a key for ecological success and protection of plants. C R Biologies, 327: 639–648.
Sheadrer CA, Descals E, Kohlmeyer B, Kohlmeyer J, Marvanová L, Padgett D, Porter D, Raja HA, Schmit JP, Thorton HA, Voglymayr H. 2007. Fungal biodiversity in aquatic babitats. Biodivers Conserv, 16: 49–67.
Suryanarayanan TS, Kumaresan V, Johnson JA. 1998. Foliar fungal endophytes from two species of the mangrove Rhizophora. Microbiol, 44: 1003–1006.
Swofford DL. 2002. PAUP*: Phylogenetic Analysis Using Parsimony (*and other methods) Version 4.0b10. Sinauer Associates, Sunderland, MA.
Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. 1997. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucl Acids Res, 24: 4876–4882.
Tucker CM. 1935. Diaporthe phaseolorum on pepper fruits. Mycologia, 27(6): 580–585.
Uddin W, Stevenson KL, Pardo-schultheiss RA, Rehner SA. 1998. Pathogenic and molecular characterization of three Phomopsis isolates from peach, plum and Asian pear. Plant Dis, 82: 732–737.
Van Rensburg JCJ, Lamprecht SC, Groenewald JZ, Castlebury LA, Crous PW. 2006. Characterization of Phomopsis spp. associated with die-back of rooibos (Aspalathus linearis) in South Africa. Stud Mycol, 55: 65–74.
Wang Z, Binder M, Hibbett DS. 2005. Life history and systematics of the aquatic discomycete Mitrula (Helotiales, Ascomycota) based on cultural, morphological, and molecular studies. Amer J Bot, 92(9): 1565–1574.
Wu XY, Liu XH, Jiang GC, Lin YC, Chan W, Vrijmoed LLP. 2005. Xyloketal G, a novel metabolite from the mangrove fungus Xylaria sp. 2508. Chem Nat Compd, 41(1): 27–29.
Yuan KP, Vrijmoed LLP, Feng MG. 2005. Survey of coastal mangrove fungi for xylanase production and optimized culture and assay conditions. Acta Microbiologica Sinica. 45(1): 91–96.
Zeng XB, Wang HY, He LY, Lin YC, Li ZT. 2006. Medium optimization of carbon and nitrogen sources for the production of eucalyptene A and xyloketal A from Xylaria sp. 2508 using response surface methodology. Process Biochem, 41:293–298.
Zhang AW, Hartman GL, Riccioni L, Chen WD, Ma RZ, Pedersen WL. 1997. Using PCR to distinguish Diaporthe phaseolorum and Phomopsis logicolla from other soybean fungal pathogens and to detect them in soybean tissues. Plant Dis, 81: 1143–1149.
Zhang AW, Riccioni L, Pedersen WL, Kollipara KP, Hartman GL. 1998. Molecular identification and phylogenetic grouping of Diaporthe phaseolorum and Phomopsis longicolla isolates from soybean. Phtopathology, 88: 1306–1314.
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Foundation item: The search was supported partly by the Guangzhou Natural Science Foundation (Grant No. 2007Z3-EO581), the Guangdong Provincial Natural Science Foundation (Grant No. 2007A0200300001-7), the Chinese High-Tech 863 Project (Grant No. 2006AA09Z422), and the National Natural Science Foundation of China (Grant No. 20572136).
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Cheng, Zs., Tang, Wc., Xu, Sl. et al. First report of an endophyte (Diaporthe phaseolorum var. sojae) from Kandelia candel . Journal of Forestry Research 19, 277–282 (2008). https://doi.org/10.1007/s11676-008-0049-9
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DOI: https://doi.org/10.1007/s11676-008-0049-9