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A microsatellite study on outcrossing rates and contamination in an Eucalyptus globulus breeding arboretum

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Abstract

Four pairs of microsatellite molecular polymorphism primers were used to analyse microsatellite fingerprints of 188 seedlings derived from an open-pollinated progeny grafted Eucalyptus globulus breeding arboretum in Victoria, south-eastern Australia. The microsatellite loci chosen for this study were highly polymorphic with the mean number of alleles per locus of 14.25. Individual mothers varied in their outcrosssing rate estimate from 15% to 95%, the overall outcrossing level in the arboretum was 47.9% and the contamination rate was 17.6%. The high selfing level was likely to result in marked inbreeding depression in the performance of open-pollinated seed lots. Open-pollinated seeds collected from such arboreta are not advisable because of its low genetic quality, although such arboreta may be useful for the seed production through large-scale manual pollination or collecting seeds only from trees or genotypes within the arboretum that have high outcrossing rates.

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Authors and Affiliations

  1. Hunan Provincial Forest Botanic Garden, Changsha, 410116, P. R. China

    Hong-xin Rao

  2. School of Plant Science and Cooperative Research Centre for Forestry, University of Tasmania, Private Bag 55, HOBART, 7001, Tasmania, Australia

    Briony Patterson, Brad Potts & Réne Vaillancourt

Authors
  1. Hong-xin Rao
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  2. Briony Patterson
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  3. Brad Potts
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  4. Réne Vaillancourt
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Corresponding author

Correspondence to Hong-xin Rao.

Additional information

Foundation items: This study was supported by State Administration of Foreign Experts Affairs, P. R. China (No.2001430007).

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Rao, Hx., Patterson, B., Potts, B. et al. A microsatellite study on outcrossing rates and contamination in an Eucalyptus globulus breeding arboretum. Journal of Forestry Research 19, 136–140 (2008). https://doi.org/10.1007/s11676-008-0023-6

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  • DOI: https://doi.org/10.1007/s11676-008-0023-6

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