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Establishment of genetic transformation system via Agrobacterium in tall fescue cultivar

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Abstract

Tall fescue (Festuca arundinacea Schreb.) is a cool-season turfgrass used on fairways in golf courses. The object of this study was to develop a more efficient, reliable, and repeatable approach in transforming the grass using Agrobacterium (EHA105), where β-glucuronidase gene (uidA) was used as a reporter and hygromycin phosphotransferase gene (hyg) as a selectable marker. An effective expression of transgene was observed in transforming 2-month-old calli derived from mature seeds (cv. Bingo) cultured on MS medium supplemented with 9 mg·L−1 2, 4-D. A two-step solid medium selection with increasing hygromycin concentration (from 30 to 50 mg·L−1) was used to obtain resistant calli. Transgenic plants have been produced from many independent transformed calli. The presence of functional β-glucuronidase gene (uidA) was detected in hygromycin-resistant calli. Transgenic plants were regenerated and PCR and Southern blot confirmed transgene integration in the tall fescue genome.

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Authors and Affiliations

  1. College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, 310014, China

    Qian Hai-feng, Shaukat Ali, Hong Liang & Xu Hao

  2. National Agricultural Research Centre, Park Road, Islamabad, Pakistan

    Shaukat Ali

Authors
  1. Qian Hai-feng
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  2. Shaukat Ali
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  3. Hong Liang
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  4. Xu Hao
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Corresponding author

Correspondence to Qian Hai-feng.

Additional information

Foundation project: This paper was supported by Zhejiang Provincial Science and Technology Plan of China (Grant No. 2003C30053) and Zhejiang Provincial Natural Science Foundation of China (Grant No. Y504076).

Biography: QIAN Hai-feng (1973–), male, Ph.D., associate professor in Zhejiang University of Technology.

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Qian, Hf., Ali, S., Hong, L. et al. Establishment of genetic transformation system via Agrobacterium in tall fescue cultivar. J. of For. Res. 17, 238–242 (2006). https://doi.org/10.1007/s11676-006-0054-9

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  • DOI: https://doi.org/10.1007/s11676-006-0054-9

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