Abstract
Leaf epidermal fingerprints of six species of Rhododendron (Rh. Aureaum, Rh. dauricum, Rh. micranthum, Rh. Mucronulatum, Rh. Redowskianum, Rh. schlippenbachii) were observed by optical microscope with nail polish expression method in Key Laboratory of Forest Plant Ecology of Ministry Education China in Northeast Forestry University in 2004. The leaf morphological features including of stomata types, characters of guard cells, subsidiary cells in lower epidemis were observed. And ordinary cells (in shape and anticlinal walls feature) as well as the trichomes in both sides of the leaves are described in detail. The results showed that there were three types of stoma in six investigated Rhododendron species, from which Pericytic stomata type exists in three species (Rh. dauricum, Rh. micranthum, and Rh. mucronulatum), Anomocytic stomatal type in Rh. Redowskianum, Diacytic stomata type in Rh. aureaum and Rh. schlippenbachii. The subsidiary cells of the Pericytic and Diacytic stomata type are different in shape and surface feature between the species, respectively. The ordinary epidermal cells show a variety from quadrangular to hexagonal, polygonal or irregular in surface view, the anticlinal walls are straight or sinuose. Trichomes (gland scales) are present in the both of the leaf sides in three species (Rh. dauricum, Rh. micranthum, and Rh. mucronulatum). All of these detail leaf features show specific specificity of leave finger print for 6 rhododendrons.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Copeland, H.F. 1943. A study, anatomical and taxonomic, of the genera of Rhododendroideae Amer. Midl [J]. Naturalist, 30: 533–625.
Cox, H.T. 1948. Studies in the comparative anatomy of the Ericales. I. Ericaceae — subfamily Rhododendroideae Amer. Midl [J]. Naturalist, 39: 220–245.
Dehgan, B. 1980. Application of epidermal morphology to taxonomic delimitations in the genus Jatropha L. (Euphorbiaceae) [J]. Bot. J. Linn. Soc., 80: 257–278.
Edeoga, H.O. and Ikem, C.I. 2001. Comparative Morphology of the leaf epidermis in three species of Boerhavia I., (Nyetaginaceae) [J]. J. Pl. Anat. Morph., 1: 14–21.
Edeoga, H.O. and Ogbebor, N.O. 2001. Epidermal features of some Nigerian species of Aneilema R. BR. Commelinaceae [J]. J. Econ taxon. Bot., 19: 117–124.
Edeoga, H.O., Osawe, P.I. 1996. Cuticular studies of some Nigerian species of Senna Tourn. Ex Mill (Syn. Cassia Tourn. ExL.); Leguminosae — Caesalpinioideae [J]. Acta phytotax. Geobot, 47(1): 41–46.
Yang Hanbi, Fang Ruizheng, Jin Cunli. 1999. Flora of China, vol. 57(1) [M]. Beijing: Science Press, 19–211.
Ferzana, J., Prabhakar, M., Leelavathi, P. 1991. Folia architecture in relation to taxonomy of Malvales [J]. Asian J Pl Sci., 3(2): 17–53.
Fryns, C.E., Van, C.W. 1973. A new classification of the ontogenetic types stomatal features of stomata [J]. Bot Rev., 39: 71–138.
Glover, B.J. 2000. Differentiation in plant epidermal cells [J]. Journal of Experimental Botany, 51(344): 497–505.
Guyot. 1971. Phylogenetic and systematic value of stoma in the umbillifereae [C]. In: Heywood V H., The Biology and Chemistry of Umbelliferae. London: Academic Press, p199–214.
Hedegaard, J. 1980a. b. Morphological studies in the Genus Rhododendron [M]. Danmark: Gds Publishing House, p760.
Hong S.-P, Son S.-P. 2000. The taxonomy consideration of leaf epidermal microstructure in the tribe Rumiceae Dum. (Polygonaceae) [J]. Kor. J. Plant Tax. 30: 105–121.
Hong, S.P, Oh, I.C. 1999. The taxonomic study of leaf epidermal microstructure in the genera Polygonum L. s. str. And polygonella Michx. (Polygoneae-Polygonaceae) [J]. Kor. J. Plant Tax., 29: 75–90.
He Mingyou, Fang Mingyuan, Hu Wenguang, et al. 1994. Flora of China, vol. 57(2) [M]. Beijing: Science Press, p7–339.
Jelani, S., Leelavathi, P., Prabhakar, M. 1990. Folia epidermis in relation to Taxonomy of Cleome (Capparaceae) [J]. Asian J. Pl Sci., 2(2):13–24.
Kurashige, Y. 2001. Sectional relationships in the genus Rhododendron (Ericaceae): evidence from MATK and TRNK intron sequences Pl [J]. Syst. Evol., 228: 1–14.
Leelavathi, P., Ramayya, N., Prabhakar, M. 1980. Folia stomatal distributiob patterns in the Leguminosae and their taxonomic significance [J]. Phytomorphology, 30(2, 3): 195–204.
Liu Shene. 1955. Illustration Flora of Woody Plant in Northeast of China [M]. Beijing: Science press, pp.568. (in Chinses)
Metcalfe C.R, Chalk L. 1988. Anatomy of Dicotyledons. Systematic Anatomy of the Leaf and Stem [M]. England: Oxford Scientific Publications, p276.
Nakai. 1917. Praecursores ad Floram Sylvaticam Koreanam. VIII [J]. The Botanical Mag., 31(369): 236.
Paliwal, G.S., 1966. Paliwal, G.S. 1966. Structure Ontogeny of Stomata in some Acanthaceae [J]. Phytomorphology, 16: 527–532.
Park, K.-W. 1994. A taxonomy study of the Magnoliaceae [J]. Res. Rep. For. Res. Inst., 50: 173–190.
Prabhakar, M. 2004. Structure, Delimitation, Nomenclature and Classification of Stomata [J]. Acta Bot Sinica, 46(2): 242–252.
Rasmussen, H. 1981. Teminology and classification of stomata and stomata development [J]. Bot. J. Linn Soc., 83:199–212.
Shtromberg, A.Y.A. 1956. On the question of classification of stomatal types of dicoty ledonous plants [J]. Sci. Publ. Chem. Pharmoc. Inst., 8: 51–66.
Van Cotthem, W. 1970a. Comparative morphological study of the stomata in the Filicopsida [J]. Bull Jard Bot. Nat Belg, 40:81–239.
Van Cotthem, W. 1970b. A classification of stomatal types [J]. Bot. J. Linn Soc, 63:235–246.
Zhang Changqin. 2003. Dujuanhua (rhododendra) [M]. Beijing: China Building Industry Press, pp. 248. (in Chinese)
Author information
Authors and Affiliations
Corresponding author
Additional information
Foundation project: This work was supported by the Korea Research Foundation and The Korean Federation of Science and Technology Socities Grant funded by Korea Government (MOEHRD, Basic Research Promotion Fund), (Project No. 032-4-1).
Biography: WANG Xiu-wei (1981–), Ph. D. student in Key Laboratory of Forest Plant Ecology Ministry of Education China, Northeast Forestry University, Harbin 150040, P. R. China.
Rights and permissions
About this article
Cite this article
Wang, Xw., Mao, Zj., Choi, K. et al. Significance of the leaf epidermis fingerprint for taxonomy of Genus Rhododendron . J. of For. Res. 17, 171–176 (2006). https://doi.org/10.1007/s11676-006-0041-1
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s11676-006-0041-1