Abstract
Objective: To explore the antitumor mechanisms of whole peptidoglycan of bifidobacterium. Methods: The apoptotic cells and the positive expression of bcl-2 and bax oncoprotein were studied nude mice transplantation tumors of colorectal carcinoma by employing in situ end labeling technique and immunohistochemical staining. Results: The apoptotic cell density, the positive rate and the staining intensity of bax oncoprotein of the transplantation tumor of colorectal carcinoma in the whole peptidoglycan injection group were significantly higher when compared with the tumor control group. The positive rate of bcl-2 oncoportein in the whole peptidoglycan injection group was obviously lower than that in the tumor control group (P<0.01). Conclusion: Whole peptidoglycan of Bifidobacterium bifidum could induce cell apoptosis of nude mice transplantation tumors of colorectal carcinoma by down-regulating the expression of the bcl-2 gene and upregulating the expression of the bax gene.
Similar content being viewed by others
References
Sekine K, Watanabe SE, Ohta J, et al. Induction and activation of tumoricidal cells in vivo and in vitro by the bacterial cell wall of Bifidobacterium infantis. Bifidobacteria Microflora 1994; 13:65.
Jue J, Hu H. Isolation and purification of whole peptidoglycan from bifidobacteria. Chin J Microecol 1997; 9:10.
Tan XH, Zhang YL, Jiang B, et al. In situ end labeling detection for apoptosis in formal tissue sections. J Cytobiol 1997; 19:48.
Gavrieli Y, Sherman Y, Shmuel A et al. Identification of programmed cell death in situ via specific labeling of nuclear DNA net squares fragmentation. J Cell Biol 1992; 119:493.
Maldonado V, Melensw-Zajgla J, Ortega A. Modulation of NF-kappa B, and bcl-2 in apoptosis induced by cisplatin in Hela cells. Mutat Res 1997; 381:67.
Dlrval ZN, Milliman CL, Korsmeyer SJ, et al. Bcl-2 heterodimerizes in vivo with a conserved homology, bax, that accelerate programmed cell death. Cell 1993; 74:609.
Sasaki T, Fukami S, Namioka S, et al. Enhancement of cytotoxic activity of macrophages in mice by oral administration of peptidoglycan derived from Bifidobacterium thermophilum. J Vet Med Sci 1994; 56:1129.
Sekine K, Ohta J, Onishi M, et al. Analysis of antitumor properties of effector cells stimulated with a cell wall preparation (WPG) of Bifidobacterium infantis. Biol Pharm Bull 1995; 18:148.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Li-sheng, W., Ling-jia, P., Li, S. et al. The apoptosis of experimental colorectal carcinoma cells induced by peptidoglycan of bifidobacterium and the expression of apoptotic regulating genes. Chin. J. Cancer Res. 11, 184–187 (1999). https://doi.org/10.1007/s11670-999-0008-9
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s11670-999-0008-9