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Promoter hypermethylation of KiSS-1 gene in gastric cancer

  • Original Article
  • Published:
Chinese Journal of Cancer Research

Abstract

Objective

To investigate the association between KiSS-1 methylation and clinicopathological characteristics of gastric cancer and evaluate the role of peritoneal lavage fluid in detecting peritoneal metastases.

Methods

The methylation status of KiSS-1 gene in 40 gastric cancer specimens, the corresponding adjacent normal mucosa, lymph nodes and peritoneal lavage fluid was investigated by methylation-spcific polymerase chain reaction(MS-PCR).

Results

Aberrant methylation of KiSS-1 gene was detected in 55%(22/40) of the adjacent normal mucosa, 82.5% (33/40)of gastric cancer specimens, 80.95%(17/21) of the lymph nodes, and 42.5%(17/40) of peritoneal lavage fluid. Methylation in gastric carcinoma and lymphonode was more frequent than in non-neoplastic gastiric mucosa. Presence of KiSS-1 methylation in peritoneal lavage fluid was significantly correlated with tumor invasion (P=0.043). The accuracy of KiSS-1 methylation in peritoneal lavage fluid for diagnosing peritoneal metastasis was 70%, with a sensitivity of 77.8% and a specificity of 67.7%.

Conclusion

Aberrant methylation of KiSS-1 gene is a common event in the occurrence and progression of gastric carcinoma, which may provide useful information for the early diagnosis of peritoneal metastases and a new therapy for gastric cancer.

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Authors and Affiliations

  1. Department of Surgical Oncology, the First Affiliated Hospital, China Medical University, Shenyang, 110001, China

    Zhi Yang, Dong-Qiu Dai & Yun-Yi Du

Authors
  1. Zhi Yang
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  2. Dong-Qiu Dai
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  3. Yun-Yi Du
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Corresponding author

Correspondence to Dong-Qiu Dai.

Additional information

This work was supported by the National Natural Science Foundation of China, (No. 30572162); and the Science Foundation for High Education Program of Liaoning Province(No. 2008S240)

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Yang, Z., Dai, DQ. & Du, YY. Promoter hypermethylation of KiSS-1 gene in gastric cancer. Chin. J. Cancer Res. 22, 280–284 (2010). https://doi.org/10.1007/s11670-010-0280-8

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  • DOI: https://doi.org/10.1007/s11670-010-0280-8

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