Abstract
Objective
To investigate the expressions of cyclin D1 and p27 and DNA content in esophageal cancer and adjacent normal tissues, and to discuss the relationship between them.
Methods
The cyclinD1 and p27 were detected by immunohistochemical staining; DNA content was measured by flow cytometry.
Results
The positive expression rates of cyclinD1 and p27 in cancer were 45.8% and 33.3% respectively, the DNA content in the positive group of cyclinD1 was higher than that in the negative group of cyclinD1(1.54±0.21 versus 1.08±0.43, P<0.05), while the DNA content and SPF (S-phase fraction) in the positive group of p27 were lower than those in the negative group (1.10±0.19 and 5.56%±5.18% versus 1.66±0.28 and 19.78%±6.12%, P<0.05).
Conclusion
The data show that the expression of cyclinD1 and p27 are related to the ontogenesis and progression of esophageal cancer. The combined detection of cyclinD1, p27 and DNA content may be indicators of diagnosis and assessment of esophageal cancer.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Donnellan R, Chetty R. CyclinD1 and human neoplasia[J]. J Clin Pathol Mol Part 1998; 51: 1–7.
Wang X, Zou S. The relationship of cyclinD1 and estrogen receptor expression in the process of proliferation metastasis in breast neoplasm[J]. J Tongji Med Uni(in Chinese) 2001; 21: 231–232.
Youssef EM, Hasuma T, Morishima Y, et al. Overexpression of cyclinD1 in rat esophageal carcinogenesis model[J]. Cancer Res 1997; 88: 18–27.
Gugger M, Kappeler A, Vonlanthen S, et al. Alteration of cell cycle regulators are less frequent in advanced non-small cell lung cancer than in respectable tumors[J]. Lung Cancer 2001; 33: 229–239.
Bae DS, Cho SB, Kim YJ, et al. Aberrant expression of cyclinD1 is associated with poor prognosis in early stage cervical cancer of the uterus[J]. Gynecol Oncol 2001; 81: 341–347.
Rvanko K, Jarviene K, Paasinen-Sohns A, et al. Loss of p27kip from cyclinE/CDKI but not from cyclink1/CDK4 complexes in cells transformed by polymine biosynthetic enzymes[J]. Cancer Res 2000; 60: 5224–5233.
Schwarz JK, Bassing CH, Kovesdi I, et al. Expression of the E2F1 transcription factor overcomes type β transforming growth factor-mediated growth suppression[J]. Proc Natl Acad Sci USA 1995; 92: 483–487.
Hengst I, Reed SI. Translational control of p27kip accumulation during the cell cycle[J]. Science 1996; 271: 1851–1864.
Barata JT, Cardoso AA, Nadler LM, et al. Interleukin-7 promotes survival and cell cycle progression of T-cell acute lymphoblastic leukemia cells by down-regulating the cyclin-dependent kinase inhibitor p27 (kip1)[J]. Blood 2001; 98: 1524.
De Paola F, Vecci AM, Granato AM, et al. p27/kip1 expression in normal epithelium, benign and neoplastic breast lesions[J]. Pathol 2002; 196:26–31.
Kim DH, Lee HI, Nam ES, et al. Reduced expression of the cell-cycle inhibitor p27kip is associated with progression and lymph node metastasis of gastric carcinoma[J]. Histopathology 2000; 36: 245–251.
Author information
Authors and Affiliations
Corresponding author
Additional information
Biography: MA Ping (1960–), female, associate professor, Cancer Research Institute, The First Affiliated Hospital, China Medical University, majors in oncology.
Rights and permissions
About this article
Cite this article
Ma, P., Yin, Yq. & Wang, Xh. Combined detection of cyclin D1, p27 and DNA content in esophageal cancer. Chin. J. Cancer Res. 18, 282–285 (2006). https://doi.org/10.1007/s11670-006-0282-8
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s11670-006-0282-8