Skip to main content
SpringerLink
Log in

Expression of heparanase and its clinical significance in human non-small cell lung cancer

  • Published:
Chinese Journal of Cancer Research

Abstract

Objective: To explore the expression and significance of heparanase in non-small cell lung cancer (NSCLC) regarding prognosis and clinicopathological parameters. Methods: The expression of heparanase was assessed using immunohistochemistry staining and Western blot in 122 paraffin-embedded specimens and 38 freshly taken tissues. The relationship between heparanase expression and the clinicopathological factors was analyzed by Chi-square test, multivariate analysis and Kaplan-Meier method. Results: In the immunoreactive cells, staining was mainly located in cytoplasma and membrane. Human heparanase was highly expressed in lung cancer tissue (78.7%, 96/122) while negative in epithelia of normal lung tissues. The level of heparanase was remarkably higher in NSCLC than that in normal tissue (P=0.043). Expression of heparanase significantly correlated with TNM stage (P=0.025), lymphatic metastasis (P=0.002) and vascular invasion (P=0.0003). The patients with positive heparanase expression had a significantly shorter survival than those with negative heparanase expression (P=0.0006). In multivariate analysis, only p-TNM stage, lymphatic metastasis and vascular invasion could be considered as prognostic factors. Conclusion: Elevated level of heparanase in human non-small cell lung cancer tissues correlates with the TNM stage, invasion, metastasis and prognosis. However, heparanase expression is not an independent prognostic factor.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Voldavsky I, Friedmann Y, Elkin M, et al. Mammalian heparanase: gene cloning, expression and function in tumor progression and metastasis [J]. Nat Med 1999; 5:793–802.

    Article  Google Scholar 

  2. Hulett MD, Freeman C, Hamdorf BJ, et al. Cloning of mammalian heparanase, an important enzyme in tumor invasion and metastasis [J] Nat Med 1999; 5:803–9.

    Article  PubMed  CAS  Google Scholar 

  3. Fairbanks MB, Mildner AM, Leone JW, et al. Processing of the human heparanase precursor and evidence that the active enzyme is a heterodimer [J]. J Biol Chem 1999; 274:29587–90.

    Article  PubMed  CAS  Google Scholar 

  4. Friedmann Y, Vlodavsky I, Aingorn H, et al. Expression of heparanase in normal, dysplastic and neoplastic human colonic mucosa and stroma [J]. Am J Pathol 2000; 157:1167–75.

    PubMed  CAS  Google Scholar 

  5. Rohloff J, Zinke J, Schoppmeyer K, et al. Heparanase expression is a prognostic indicator for postoperative survival in pancreatic adenocarcinoma [J]. Br J Cancer 2002; 86:1270–5.

    Article  PubMed  CAS  Google Scholar 

  6. Maxhimer JB, Quiros RM, Stewart R, et al. Heparanase-1 expression is associated with the metastatic potential of breast cancer [J]. Surgery 2002; 132:326–33.

    Article  PubMed  Google Scholar 

  7. Endo K, Maejara U, Baba H, et al. Heparanase gene expression and metastatic potential in human gastric cancer [J]. Anticancer Res 2001; 21:3365–9.

    PubMed  CAS  Google Scholar 

  8. Tang W, Nakamura Y, Tsujimoto M, et al. Heparanase: a key enzyme in invasion and metastasis of gastric carcinoma [J]. Mod Pathol 2002; 15:593–8.

    Article  PubMed  Google Scholar 

  9. Takaoka M, Naomoto Y, Ohkawa T, et al. Heparanase expression correlates with invasion and poor prognosis in gastric cancers [J]. Lab Invest 2003; 83:613–22.

    PubMed  CAS  Google Scholar 

  10. Mikami S, Ohashi K, Usui Y, et al. Loss of syndecan-1 and increased expression of heparanase in invasive esophageal carcinomas [J]. Jpn J Cancer Res 2001; 92:1062–73.

    PubMed  CAS  Google Scholar 

  11. Kuniyasu H, Chihara Y, Kubozoe T, et al. Co-expression of CD44v3 and heparanase is correlated with metastasis of human colon cancer [J]. Int J Mol Med 2002; 10:333–7.

    PubMed  Google Scholar 

  12. Kim AW, Xu X, Hollinger EF, et al. Human heparanase-1 gene expression in pancreatic adenocarcinoma [J]. J Gastrointest Surg 2002; 6:167–72.

    Article  PubMed  Google Scholar 

  13. Koliopanos A, Friess H, Kleeff J, et al. Heparanase expression in primary and metastatic pancreatic cancer [J]. Cancer Res 2001; 61:4655–9.

    PubMed  CAS  Google Scholar 

  14. Gohji K, Okamoto M, Kitazawa S, et al. Heparanase protein and gene expression in bladder cancer [J]. J Urol 2001; 166:1286–90.

    Article  PubMed  CAS  Google Scholar 

  15. Gohji K, Hirano H, Okamoto M, et al. Expression of three extracellular matrix degradative enzyme in bladder cancer [J]. Int J Cancer 2001; 95:295–301.

    Article  PubMed  CAS  Google Scholar 

  16. Kurokawa H, Katsube K, Podyma KA, et al. Heparanase and tumor invasion patterns in human oral squamous cell carcinoma xenografts [J]. Cancer Sci 2003; 94:277–85.

    Article  PubMed  CAS  Google Scholar 

  17. Ikeguchi M, Ueta T, Yamane Y, et al. Quantitative analysis of heparanase messenger RNA expression in hepatocellular carcinoma [J]. J Surg Oncol 2002; 81:148–54.

    Article  PubMed  CAS  Google Scholar 

  18. Zhang YL, Fu ZR, Ding GS, et al. Expression of heparanase mRNA and its clinical significance in primary hepatocellular carcinoma [J]. Clin J Oncol 2003; 25:359–61.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pathology, China Medical University, 110011, Shenyang, China

    Han Yu-chen, Li Shu-yu, Yu Min, Gu Yu-mei, Zhang Si-yang, Wang En-hua & Qiu Xue-shan

  2. Insight Strategy and Marketing Ltd., Israel

    Iris Pecker

Authors
  1. Han Yu-chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Li Shu-yu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yu Min
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Gu Yu-mei
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Zhang Si-yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Iris Pecker
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Wang En-hua
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Qiu Xue-shan
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Qiu Xue-shan.

Additional information

Foundation item: This work was supported by the National Natural Sciences Foundation of China (No. 30170409) and the Scientific Research Foundation of Liaoning Education Office (No. 20122178).

Biography: HAN Yu-chen (1969–), female, doctor of medicine, associate professor, Department of Pathology, China Medical University, majors in tumor pathology.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Han, Yc., Li, Sy., Yu, M. et al. Expression of heparanase and its clinical significance in human non-small cell lung cancer. Chin. J. Cancer Res. 17, 207–212 (2005). https://doi.org/10.1007/s11670-005-0042-1

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11670-005-0042-1

Key words

CLC number

Search

Navigation