Abstract
Objective: To investigate the regulation effect of protein kinase A on IL-6-induced STAT3 activation in myeloma cells. Methods: Two human myeloma cell lines-Sko-007 and U266 were pretreated with Forskolin, a protein kinase A antagonist, and then stimulated by IL-6. The activation state of STAT3 in these two cells were examined by electrophoretic mobility shift assay (EMSA). Results: Although PKA pathway itself doesn’t participate in IL-6 signal transduction in Sko-007 and U266 cells, activation of protein kinase A can inhibit IL-6-induced STAT3 activation in these two cell lines. Conclusion: There exists an inhibitory effect of protein kinase A on STAT3 activation in human myeloma cells treated by IL-6.
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Abbreviations
- IL-6:
-
Interleukin-6
- Jak:
-
Janus kinase
- STAT:
-
signal transducer and activator of transcription
- APRE:
-
acute phase response element
- CRE:
-
cAMP responsive element
- CREB:
-
cAMP responsive element binding protein
- EMSA:
-
electrophoretic mobility shift assay
- PKA:
-
protein kinase A
- ERK:
-
extracellular signal regulated protein kinase
- FK:
-
Forskolin
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Foundation item: This work was supported by the national natural Science Foundation of China (No. 39925019)
Biography: SONG Lun(1971–), doctor of medicine. associate professor, majors in signal transduction mechanism of cytokine.
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Song, L., Li, y. & Shen, Bf. Inhibition of IL-6-induced STAT3 activation in myeloma cells by protein kinase a. Chin. J. Cancer Res. 13, 243–246 (2001). https://doi.org/10.1007/s11670-001-0039-3
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DOI: https://doi.org/10.1007/s11670-001-0039-3