Skip to main content
SpringerLink
Log in

Effects of Zn Content on Crystal Structure, Cytocompatibility, Antibacterial Activity, and Chemical Stability in Zn-Modified Calcium Silicate Coatings

  • Peer Reviewed
  • Published:
Journal of Thermal Spray Technology Aims and scope Submit manuscript

Abstract

In our previous study, Zn-modified calcium silicate coatings possess not only excellent chemical stability but also well antibacterial activity. Still, effects of zinc content on these properties and cytocompatibility remain unclear. In this paper, two kinds of Zn-modified calcium silicate coatings (ZC0.3, ZC0.5) were fabricated on Ti-6Al-4V substrates via plasma spraying technology. X-ray diffraction results and transmission electron microscopy observations showed that the ZC0.5 coating was composed of pure hardystonite (Ca2ZnSi2O7) phase, while, besides Ca2ZnSi2O7 phase, the amorphous CaSiO3 phase was also detected in the ZC0.3 coating. Chemical stability in Tris-HCl buffer solution and antibacterial activity of the Zn-modified calcium silicate coatings increased with an increase in zinc content. In vitro cytocompatibility evaluation demonstrated that the proliferation and alkaline phosphatase activity and collagen type I (COLI) secretion of osteoblast-like MC3T3-E1 cells on Zn-modified coatings were significantly enhanced compared to the Zn-free coating and Ti-6Al-4V control, and no cytotoxicity appeared on Zn-modified coatings. The better antibacterial activity and the enhanced capability to promote MC3T3-E1 cells differentiation of Zn-modified coatings should be attributed to the slow and constant Zn2+ releasing from the coatings.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9
Fig. 10

Similar content being viewed by others

References

  1. M. Browne and P.J. Gregson, Effect of Mechanical Surface Pretreatment on Metal Ion Release, Biomaterials, 2000, 21(4), p 385-392

    Article  CAS  Google Scholar 

  2. L.M. Sun, C.C. Berndt, K.A. Gross, and A. Kucuk, Material Fundamentals and Clinical Performance of Plasma-Sprayed Hydroxyapatite Coatings: A Review, J. Biomed. Mater. Res., 2001, 58(5), p 570-592

    Article  CAS  Google Scholar 

  3. W.C. Xue, X.Y. Liu, X.B. Zheng, and C.X. Ding, In Vivo Evaluation of Plasma-Sprayed Wollastonite Coating, Biomaterials, 2005, 26(17), p 3455-3460

    Article  CAS  Google Scholar 

  4. J.Y. Sun, L. Wei, X.Y. Liu, J.Y. Li, B.E. Li, G.C. Wang, and F.H. Meng, Influences of Ionic Dissolution Products of Dicalcium Silicate Coating on Osteoblastic Proliferation, Differentiation and Gene Expression, Acta Biomater., 2009, 5(4), p 1284-1293

    Article  CAS  Google Scholar 

  5. X.Y. Liu, S.Y. Tao, and C.X. Ding, Bioactivity of Plasma Sprayed Dicalcium Silicate Coatings, Biomaterials, 2002, 23(3), p 963-968

    Article  CAS  Google Scholar 

  6. C.T. Wu, Y. Ramaswamy, J. Chang, J. Woods, Y.Q. Chen, and H. Zreiqat, The Effect of Zn Contents on Phase Composition, Chemical Stability and Cellular Bioactivity in Zn-Ca-Si System Ceramics, J. Biomed. Mater. Res. B, 2008, 87(2), p 346-353

    Google Scholar 

  7. Y. Liang, Y.T. Xie, H. Ji, L.P. Huang, and X.B. Zheng, Excellent Stability of Plasma-Sprayed Bioactive Ca3ZrSi2O9 Ceramic Coating on Ti-6Al-4V, Appl. Surf. Sci., 2010, 256(14), p 4677-4681

    Article  CAS  Google Scholar 

  8. X.Y. Liu and C.X. Ding, Plasma Sprayed Wollastonite/TiO2 Composite Coatings on Titanium Alloys, Biomaterials, 2002, 23(20), p 4065-4077

    Article  CAS  Google Scholar 

  9. K. Li, J. Yu, Y. Xie, L. Huang, X. Ye, and X. Zheng, Chemical Stability and Antimicrobial Activity of Plasma Sprayed Bioactive Ca2ZnSi2O7 Coating, J. Mater. Sci. Mater. Med., 2011, 22(12), p 2781-2789

    Article  CAS  Google Scholar 

  10. R.L. Du, J. Chang, S.Y. Ni, W.Y. Zhai, and J.Y. Wang, Characterization and In Vitro Bioactivity of Zinc-Containing Bioactive Glass and Glass-Ceramics, J. Biomater. Appl., 2006, 20(4), p 341-360

    Article  CAS  Google Scholar 

  11. H. Zreiqat, Y. Ramaswamy, C.T. Wu, A. Paschalidis, Z.F. Lu, B. James, O. Birke, M. McDonald, D. Little, and C.R. Dunstan, The Incorporation of Strontium and Zinc into a Calcium-Silicon Ceramic for Bone Tissue Engineering, Biomaterials, 2010, 31(12), p 3175-3184

    Article  CAS  Google Scholar 

  12. A. Ito, H. Kawamura, M. Otsuka, M. Ikeuchi, H. Ohgushi, K. Ishikawa, K. Onuma, N. Kanzaki, Y. Sogo, and N. Ichinose, Zinc-Releasing Calcium Phosphate for Stimulating Bone Formation, Mater. Sci. Eng. C., 2002, 22(1), p 21-25

    Article  Google Scholar 

  13. V. Aina, G. Malavasi, A.F. Pla, L. Munaron, and C. Morterra, Zinc-Containing Bioactive Glasses: Surface Reactivity and Behaviour Towards Endothelial Cells, Acta Biomater., 2009, 5(4), p 1211-1222

    Article  CAS  Google Scholar 

  14. G. Lusvardi, G. Malavasi, L. Menabue, M.C. Menziani, A. Pedone, U. Segre, V. Aina, A. Perardi, C. Morterra, F. Boccafoschi, S. Gatti, M. Bosetti, and M. Cannas, Properties of Zinc Releasing Surfaces for Clinical Applications, J. Biomater. Appl., 2008, 22(6), p 505-526

    Article  CAS  Google Scholar 

  15. Y.K. Chen, X.B. Zheng, Y.T. Xie, C.X. Ding, H.J. Ruan, and C.Y. Fan, Anti-bacterial and Cytotoxic Properties of Plasma Sprayed Silver-Containing HA Coatings, J. Mater. Sci.-Mater. Med., 2008, 19(12), p 3603-3609

    Article  CAS  Google Scholar 

  16. P.G. Wu, J.A. Imlay, and J.K. Shang, Mechanism of Escherichia coli Inactivation on Palladium-Modified Nitrogen-Doped Titanium Dioxide, Biomaterials, 2010, 31(29), p 7526-7533

    Article  CAS  Google Scholar 

  17. Q.L. Feng, J. Wu, G.Q. Chen, F.Z. Cui, T.N. Kim, and J.O. Kim, A Mechanistic Study of the Antibacterial Effect of Silver Ions on Escherichia coli and Staphylococcus aureus, J. Biomed. Mater. Res., 2000, 52(4), p 662-668

    Article  CAS  Google Scholar 

  18. K. Li, J.M. Yu, Y.T. Xie, L.P. Huang, X.J. Ye, and X.B. Zheng, Chemical Stability and Antimicrobial Activity of Plasma Sprayed Bioactive Ca2ZnSi2O7 Coating, J. Mater. Sci.-Mater. Med., 2011, 22(12), p 2781-2789

    Article  CAS  Google Scholar 

  19. H. Hu, W. Zhang, Y. Qiao, X. Jiang, X. Liu, and C. Ding, Antibacterial Activity and Increased Bone Marrow Stem Cell Functions of Zn-Incorporated TiO2 Coatings on Titanium, Acta Biomater., 2012, 8(2), p 904-915

    Article  CAS  Google Scholar 

  20. S. Samani, S.M. Hossainalipour, M. Tamizifar, and H.R. Rezaie, In Vitro Antibacterial Evaluation of Sol-Gel-Derived Zn-, Ag-, and (Zn + Ag)-Doped Hydroxyapatite Coatings Against Methicillin-Resistant Staphylococcus aureus, J. Biomed. Mater. Res. A, 2012, 101, p 222-230

    Google Scholar 

  21. S.D. Miao, K. Cheng, W.J. Weng, P.Y. Du, G. Shen, G.R. Han, W.Q. Yan, and S. Zhang, Fabrication and Evaluation of Zn Containing Fluoridated Hydroxyapatite Layer with Zn Release Ability, Acta Biomater., 2008, 4(2), p 441-446

    Article  CAS  Google Scholar 

  22. Y. Ramaswamy, C.T. Wu, H. Zhou, and H. Zreiqat, Biological Response of Human Bone Cells to Zinc-Modified Ca-Si-Based Ceramics, Acta Biomater., 2008, 4(5), p 1487-1497

    Article  CAS  Google Scholar 

  23. J. Ovesen, B. Moller-Madsen, J.S. Thomsen, G. Danscher, and L. Mosekilde, The Positive Effects of Zinc on Skeletal Strength in Growing Rats, Bone, 2001, 29(6), p 565-570

    Article  CAS  Google Scholar 

  24. M. Kamitakahara, C. Ohtsuki, H. Inada, M. Tanihara, and T. Miyazaki, Effect of ZnO Addition on Bioactive CaO-SiO2-P2O5-CaF2 Glass-Ceramics Containing Apatite and Wollastonite, Acta Biomater., 2006, 2(4), p 467-471

    Article  CAS  Google Scholar 

  25. W.H. Song, J.Y. Zhang, J. Guo, J.H. Zhang, F. Ding, L.Y. Li, and Z.T. Sun, Role of the Dissolved Zinc Ion and Reactive Oxygen Species in Cytotoxicity of ZnO Nanoparticles, Toxicol. Lett., 2010, 199(3), p 389-397

    Article  CAS  Google Scholar 

  26. A. Yamamoto, R. Honma, and M. Sumita, Cytotoxicity Evaluation of 43 Metal Salts Using Murine Fibroblasts and Osteoblastic Cells, J. Biomed. Mater. Res., 1998, 39(2), p 331-340

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grant No. 81071455, No. 51172264) and the Foundation of Shanghai Biomaterial and Clinic Research Centre (Grant No. BMCRC2010004). We would like to acknowledge the support of the National Natural Science Foundation of China (Grant No. 51232007).

Author information

Authors and Affiliations

  1. Key Laboratory of Inorganic Coating Materials, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai, 200050, People’s Republic of China

    Kai Li, Youtao Xie, Liping Huang & Xuebin Zheng

  2. Department of Orthopaedics, Changzheng Hospital, Second Military Medical University, Shanghai, 200003, People’s Republic of China

    Jiangming Yu & Xiaojian Ye

Authors
  1. Kai Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jiangming Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Youtao Xie
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Liping Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Xiaojian Ye
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Xuebin Zheng
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Xuebin Zheng.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Li, K., Yu, J., Xie, Y. et al. Effects of Zn Content on Crystal Structure, Cytocompatibility, Antibacterial Activity, and Chemical Stability in Zn-Modified Calcium Silicate Coatings. J Therm Spray Tech 22, 965–973 (2013). https://doi.org/10.1007/s11666-013-9938-3

Download citation

  • Received:

  • Revised:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11666-013-9938-3

Keywords

Search

Navigation