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Electropolymerization of Stable Leucoemeraldine Base Polyaniline Film and Application for Quantitative Detection of Escherichia coli O157:H7

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Abstract

A stable leucoemeraldine base polyaniline (PANI) has been optimally electrodeposited on a bare screen-printed carbon electrode (SPCE) using a potentiostatic method and a nonenzymatic electrochemical immunosensor for quantitative detection of Escherichia coli O157:H7 (E. coli O157:H7) fabricated. The stability, electroactivity, and surface morphology of the PANI film were studied by cyclic voltammetry and scanning electron microscopy. The PANI film formed by the potentiostatic method was firmly attached to the SPCE, being more stable than PANI film electrodeposited by a cyclic voltammetric method. Gold nanoparticles (AuNPs) were linked to the PANI film and used as a connector to an antibody against E. coli O157:H7 (Ab). E. coli O157:H7 was quantitatively detected by differential pulse voltammetry based on the PANI/Ab-modified SPCE. The principle of this quantitative method is based on a prominent decrease of the current after specific binding to E. coli O157:H7. Under the optimized conditions, a linear relationship between the peak current change (ΔI) and the logarithm of the E. coli O157:H7 concentration was obtained in the range from 4.0 × 104 colony-forming units (CFU)/mL to 4.0 × 109 CFU/mL, with limit of detection (LOD) of 7.98 × 103 CFU/mL at signal-to-noise ratio (S/N) of 3. The immunoassay exhibited acceptable specificity, reproducibility, and stability for detection of E. coli O157:H7. In future work, the proposed approach could be used to prepare stable films which can withstand sonication and strong acid.

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References

  1. Q. Zhou, Y. Lin, K. Zhang, M. Li, and D. Tang, Biosens. Bioelectron. 101, 146 (2017).

    Article  Google Scholar 

  2. K. Zhang, S. Lv, Z. Lin, M. Li, and D. Tang, Biosens. Bioelectron. 101, 159 (2017).

    Article  Google Scholar 

  3. D. Tang and J. Shu, Chem. Asian J. 12, 2780 (2017).

    Article  Google Scholar 

  4. Z. Qiu, J. Shu, and D. Tang, Anal. Chem. 90, 1021 (2017).

    Article  Google Scholar 

  5. J. Shu, Z. Qiu, S. Lv, K. Zhang, and D. Tang, Anal. Chem. 90, 2425 (2018).

    Article  CAS  Google Scholar 

  6. L. Bai, R. Yuan, Y. Chai, Y. Yuan, L. Mao, and Y. Wang, Anal. Chim. Acta 698, 14 (2011).

    Article  CAS  Google Scholar 

  7. S. Huang, S. Lu, C. Huang, J. Sheng, L. Zhang, W. Su, and Q. Xiao, Sens. Actuators, B 224, 22 (2016).

    Article  CAS  Google Scholar 

  8. A. Weremfo, T.C.F. Sui, A. Khan, D.B. Hibbert, and C. Zhao, Electrochim. Acta 231, 20 (2017).

    Article  CAS  Google Scholar 

  9. C. Hu, W. Dou, and G. Zhao, Electrochim. Acta 117, 239 (2014).

    Article  CAS  Google Scholar 

  10. P.Y. Cheung and M.K. Kai, Food Res. Int. 45, 802 (2012).

    Article  CAS  Google Scholar 

  11. C. Yi, J.H. Lee, B.S. Kwak, M.X. Lin, H.O. Kim, and H.I. Jung, Sens. Actuators, B 191, 305 (2014).

    Article  CAS  Google Scholar 

  12. J.J. Shi, T.T. He, F. Jiang, E.S. Abdelhalim, and J.J. Zhu, Biosens. Bioelectron. 55, 51 (2014).

    Article  CAS  Google Scholar 

  13. A.P. Washe and P. Lozano, Electrochim. Acta 91, 166 (2013).

    Article  CAS  Google Scholar 

  14. R. Pallela, P. Chandra, H.B. Noh, and Y.B. Shim, Biosens. Bioelectron. 85, 883 (2016).

    Article  CAS  Google Scholar 

  15. M.J. Monerris, F. D´Eramo, F.J. Arévalo, H. Fernández, M.A. Zon, and P.G. Molina, Microchem. J. 129, 71 (2016).

    Article  CAS  Google Scholar 

  16. D. Wang, W. Dou, G. Zhao, and Y. Chen, J. Microbiol. Methods 106, 110 (2014).

    Article  CAS  Google Scholar 

  17. Y.S. Fang, H.Y. Wang, L.S. Wang, and J.F. Wang, Biosens. Bioelectron. 51, 310 (2014).

    Article  CAS  Google Scholar 

  18. F. Liu, G. Xiang, D. Jiang, L. Zhang, X. Chen, L. Liu, F. Luo, Y. Li, C. Liu, and X. Pu, Biosens. Bioelectron. 74, 214 (2015).

    Article  CAS  Google Scholar 

  19. L. Zhao, S. Li, J. He, G. Tian, Q. Wei, and H. Li, Biosens. Bioelectron. 49, 222 (2013).

    Article  CAS  Google Scholar 

  20. G. Fusco, F. Gallo, C. Tortolini, P. Bollella, F. Ietto, M.A. De, A. D’Annibale, R. Antiochia, G. Favero, and F. Mazzei, Biosens. Bioelectron. 93, 52 (2016).

    Article  Google Scholar 

  21. Y. Su, H. Zhu, and H. Dong, Anal. Lett. 48, 477 (2015).

    Article  CAS  Google Scholar 

  22. P. Si, H. Chen, P. Kannan, and D.H. Kim, Analyst 136, 5134 (2011).

    Article  CAS  Google Scholar 

  23. H. Ni, X. Sun, Z. Song, S. Niu, and X. Luo, Biosens. Bioelectron. 86, 143 (2016).

    Article  Google Scholar 

  24. Y. Zhang and G.C. Rutledge, Macromolecules 45, 4238 (2012).

    Article  CAS  Google Scholar 

  25. J.E.D. Albuquerque, L.H.C. Mattoso, R.M. Faria, J.G. Masters, and A.G. Macdiarmid, Synth. Met. 146, 1 (2004).

    Article  Google Scholar 

  26. C. Dhand, M. Das, M. Datta, and B.D. Malhotra, Biosens. Bioelectron. 26, 2811 (2011).

    Article  CAS  Google Scholar 

  27. H.F. Jiang and X.X. Liu, Electrochim. Acta 55, 7175 (2010).

    Article  CAS  Google Scholar 

  28. A. Parsa and S.A. Ghani, Polymer 49, 3702 (2008).

    Article  CAS  Google Scholar 

  29. Y. Zheng, H. Wang, and Z. Ma, Microchim. Acta 184, 4269 (2017).

    Article  CAS  Google Scholar 

  30. W. Yan, X. Chen, X. Li, X. Feng, and J.J. Zhu, J. Phys. Chem. B 112, 1275 (2008).

    Article  CAS  Google Scholar 

  31. M. Bartoszek, M. Wecks, G. Jakobs, and D. Möhlmann, E. Flammarion 160, 1070 (2011).

    Google Scholar 

  32. R.J. Klingler and J.K. Kochi, J. Phys. Chem. 85, 1731 (2002).

    Article  Google Scholar 

  33. K. Bade, V. Tsakova, and J.W. Schultze, Electrochim. Acta 37, 2255 (1992).

    Article  CAS  Google Scholar 

  34. R. Córdova, M.A.D. Valle, A. Arratia, H. Gómez, and R. Schrebler, J. Electroanal. Chem. 377, 75 (1994).

    Article  Google Scholar 

  35. Y. Wang, Z. Yong, S. Yu, L. Feng, H. Ma, L. He, B. Du, and W. Qin, Talanta 124, 60 (2014).

    Article  CAS  Google Scholar 

  36. L. Yang, H. Zhao, S. Fan, S. Deng, Q. Lv, J. Lin, and C.P. Li, Biosens. Bioelectron. 57, 199 (2014).

    Article  CAS  Google Scholar 

  37. P.K. Vabbina, A. Kaushik, N. Pokhrel, S. Bhansali, and N. Pala, Biosens. Bioelectron. 63, 124 (2015).

    Article  CAS  Google Scholar 

  38. S.M. Radke and E.C. Alocilja, Biosens. Bioelectron. 20, 1662 (2005).

    Article  CAS  Google Scholar 

  39. Y. Wang, Z. Ye, C. Si, and Y. Ying, Sensors. 11, 2728 (2011).

    Article  CAS  Google Scholar 

  40. A. Wolter, R. Niessner, and M. Seidel, Anal. Chem. 80, 5854 (2008).

    Article  CAS  Google Scholar 

  41. L.C. Shriver-Lake, S. Turner, and C.R. Taitt, Anal. Chim. Acta 584, 66 (2007).

    Article  CAS  Google Scholar 

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Acknowledgements

The authors acknowledge support from the National Natural Science Foundation of Zhejiang Province (LY17C200003), the Food and Engineering Most Important Discipline of Zhejiang Province (2017SIAR210, JYTSP20141062), Zhejiang Public Innovation Platform Analysis and Testing Project (2018C37056), Plans of College Students in Zhejiang Province and Technology Innovation Activities (acrobatic tender grass talent program) Project (1110KZN0217054G, 1110KZN0217053G), Open Fund of State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, and The First Affiliated Hospital of Medical College, Zhejiang University (2017KF02).

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Authors and Affiliations

  1. Food Safety Key Laboratory of Zhejiang Province, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, 310018, China

    Xiaoyan Mo, Guangying Zhao & Wenchao Dou

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  1. Xiaoyan Mo
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  2. Guangying Zhao
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  3. Wenchao Dou
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Correspondence to Guangying Zhao or Wenchao Dou.

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Mo, X., Zhao, G. & Dou, W. Electropolymerization of Stable Leucoemeraldine Base Polyaniline Film and Application for Quantitative Detection of Escherichia coli O157:H7. J. Electron. Mater. 47, 6507–6517 (2018). https://doi.org/10.1007/s11664-018-6517-x

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  • DOI: https://doi.org/10.1007/s11664-018-6517-x

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