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Baicalin Reduces Early Brain Injury after Subarachnoid Hemorrhage in Rats

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Abstract

Objective

To evaluate the effect of baicalin on subarachnoid hemorrhage (SAH) in rats and explore the potential mechanisms.

Methods

Sprague-Dawley rats underwent experimental SAH and received treatment with baicalin at 10 or 50 mg/kg after 2 and 12 h of SAH. Neurological scores, brain water content, Evans-blue extravasation, and levels of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), myeloperoxidase (MPO), and malondialdehyde (MDA) were measured 24 h after SAH. Expression of nuclear factor erythroid-related factor 2 (Nrf2), NAD(P)H: quinone oxidoreductase 1 (NQO1), matrix metalloproteinase-9 (MMP-9), aquaporin 4 (AQP4), occludin, and zonulaoccludens-1 (ZO-1) were detected in the brain by Western blot. Heme oxygenase-1 (HO-1) was detected by quantitative polymerase chain reaction, and tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) were assessed by enzyme-linked immunosorbent assay.

Results

Baicalin attenuated EBI 24 h after SAH in rats (P<0.05). Baicalin elevated neurological scores, GSH-Px, SOD, and increased the expression of Nrf2, NQO1, HO-1, occludin, and ZO-1 in SAH rats (P<0.05 or P<0.01). Baicalin reduced MPO, MDA, and the expression of MMP-9, AQP4, TNF-α, and IL-1β (P<0.05 or P<0.01).

Conclusion

Baicalin reduced SAH-induced EBI, partially via activation of the Nrf2/HO-1 pathway and inhibition of MMP-9 and AQP4.

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Author information

Authors and Affiliations

  1. Department of Neurosurgery, Fujian Medical University Union Hospital, Neurosurgery Research Institute of Fujian Province, Fuzhou, 350001, China

    Hua-bin Zhang, Xian-kun Tu, Shi-wei Song, Ri-sheng Liang & Song-sheng Shi

Authors
  1. Hua-bin Zhang
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  2. Xian-kun Tu
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  3. Shi-wei Song
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  4. Ri-sheng Liang
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  5. Song-sheng Shi
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Contributions

Tu XK designed the experiments and drafted the manuscript. Shi SS helped perform the analysis with constructive discussions and revised the manuscript. Zhang HB and Song SW carried out the experiments. Liang RS contributed to the statistical analysis. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Xian-kun Tu.

Ethics declarations

The authors declare that they have no competing interests.

Additional information

Supported by the Project of Medical Innovation of Health and Family Planning Commision of Fujian Province of China (No. 2016-CX-18), the Natural Science Foundation of Fujian Province of China (No. 2017J01204), the Key Young Talents Cultivation Project of Health and Family Planning Commision of Fujian Province of China (No. 2016-ZQN-28), the Excellent Youth Scientific Research Personnel Cultivation Program of Fujian Province’s High School (No. 2016B026), the Clinical Key Subject (Neurosurgery) Foundation of Fujian Medical University, and the Key Department (Neurosurgery) Foundation from Fujian Medical University Union Hospital, China

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Zhang, Hb., Tu, Xk., Song, Sw. et al. Baicalin Reduces Early Brain Injury after Subarachnoid Hemorrhage in Rats. Chin. J. Integr. Med. 26, 510–518 (2020). https://doi.org/10.1007/s11655-020-3183-7

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  • DOI: https://doi.org/10.1007/s11655-020-3183-7

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