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Ethanol Extract of Lycopodium serratum Thunb. Attenuates Lipopolysaccharide-Induced C6 Glioma Cells Migration via Matrix Metalloproteinase-9 Expression

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Abstract

Objective

To elucidate how ethanol extract of L. serratum (ELS) could exert anti-migratory effects on glioma with the suppression of nuclear factor kappa B (NF-κB) downstream pathway.

Methods

Cell viability of ELS on C6 glioma was detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Nitric oxide (NO) assay and 2′,7′-dichlorofluorescin diacetate (DCFH-DA) assay were applied to measure NO production and reactive oxygen species (ROS) generation on lipopolysaccharide (LPS)-induced C6 glioma cells. NF-κB, mitogen-activated protein kinase (MAPK), inducible nictric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) protein were determined by Western blot. Wound healing assay was used to investigate the inhibitory effect of ELS on fetal bovine serum (FBS)-induced migration and matrix metalloproteinase (MMP)-9 and -2 activity was examined by zymography.

Results

ELS suppressed LPS-induced phosphorylation of extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), and p38 through inhibiting the expression of chemokine CCL2 (or monocyte chemoattractant protein-1, MCP-1). In addition, ELS inhibited the expression of iNOS, COX-2, and the production of NO by LPS in C6 glioma cells. ELS also significantly decreased serum-induced migration of C6 glioma cells in scratch wound healing in a dose-dependent manner (P<0.01). The activity of MMP-9 and -2 were also significantly attenuated by ELS with LPS treatment (P<0.01).

Conclusions

Our results suggest that downregulation of MMP-9 gene expression might be involved in the anti-migration effect of ELS against LPS-induced C6 glioma cells.

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Authors and Affiliations

  1. Institute of Korean Medicine, College of Korean Medicine, Dongguk University, Dongguk-Ro 32, Goyang, 10326, Republic of Korea

    Ju-Yeon Park

  2. Department of Diagnostics, College of Korean Medicine, Dongguk University, Dongguk-Ro 32, Goyang, 10326, Republic of Korea

    Hyuck Kim & Won-Hwan Park

  3. Department of Pathology, College of Korean Medicine, Dongguk University, Dongguk-Ro 32, Goyang, 10326, Republic of Korea

    Dong-Woo Lim & Jai-Eun Kim

  4. Department of Prescription, College of Korean Medicine, Dongguk University, Dongguk-Ro 32, Goyang, 10326, Republic of Korea

    Sun-Dong Park

Authors
  1. Ju-Yeon Park
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  2. Hyuck Kim
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  3. Dong-Woo Lim
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  4. Jai-Eun Kim
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  5. Won-Hwan Park
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  6. Sun-Dong Park
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Correspondence to Sun-Dong Park.

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Supported by Dongguk University Research Fund of 2015

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Park, JY., Kim, H., Lim, DW. et al. Ethanol Extract of Lycopodium serratum Thunb. Attenuates Lipopolysaccharide-Induced C6 Glioma Cells Migration via Matrix Metalloproteinase-9 Expression. Chin. J. Integr. Med. 24, 860–866 (2018). https://doi.org/10.1007/s11655-017-2923-9

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  • DOI: https://doi.org/10.1007/s11655-017-2923-9

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