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Chinese Journal of Integrative Medicine

, Volume 24, Issue 5, pp 353–358 | Cite as

Effects of Ligustrazine on Airway Inflammation in A Mouse Model of Neutrophilic Asthma

  • Xiao-ming Liu
  • Yong-bin Wang
  • Qian Wu
  • Zhong-rui Bian
  • Xiao-wen Che
Original Article

Abstract

Objective

To investigate the effects of ligustrazine (LTZ) on airway inflammation in a mouse model of neutrophilic asthma (NA).

Methods

Forty healthy C57BL/6 female mice were randomly divided into 4 groups using a random number table, including the normal control, NA, LTZ and dexamethasone (DXM) groups, with 10 rats in each group. The NA mice model was established by the method of ovalbumin combined with lipopolysaccharide sensitization. At 0.5 h before each challenge, LTZ and DXM groups were intraperitoneally injected with LTZ (80 mg/kg) or DXM (0.5 mg/kg) for 14 d, respectively, while the other two groups were given the equal volume of normal saline. After last challenge for 24 h, the aerosol inhalation of methacholine was performed and the airway reactivity was measured. The bronchoalveolar lavage fluid (BALF) was collected. The Wright-Giemsa staining was used for total white blood cells and differential counts. The levels of cytokines interleukin (IL)-17 and IL-10 were detected by enzyme-linked immunosorbent assay. The pathological change of lung tissue was observed by hematoxylin eosin staining.

Results

The airway responsiveness of the NA group was signifificantly higher than the normal control group (P<0.05), while those in the LTZ and DXM groups were signifificantly lower than the NA group (P<0.05). The neutrophil and eosinophil counts in the LTZ and DXM groups were signifificantly lower than the NA group (P<0.05), and those in the LTZ group were signifificantly lower than the DXM group (P<0.05). There were a large number of peribronchiolar and perivascular inflammatory cells in fifiltration in the NA group. The airway inflflammation in the LTZ and DXM groups were signifificantly alleviated than the NA group. The infifiltration in the LTZ group was signifificantly reduced than the DXM group. Compared with the normal control group, the IL-17 level in BALF was signifificantly increased and the IL-10 level in BALF was signifificantly decreased in the NA group (P<0.05). LTZ and DXM treatment signifificantly decreased IL-17 levels and increased IL-10 levels compared with the NA group (P<0.05), and the changes in the above indices were more signifificant in the LTZ group (P<0.05).

Conclusion

LTZ could alleviate the airway inflflammation in the NA mice model through increasing the IL-10 level and decreasing the IL-17 level.

Keywords

ligustrazine neutrophilic asthma airway inflflammation cytokine 

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Copyright information

© Chinese Association of the Integration of Traditional and Western Medicine 2017

Authors and Affiliations

  • Xiao-ming Liu
    • 1
  • Yong-bin Wang
    • 2
  • Qian Wu
    • 2
  • Zhong-rui Bian
    • 2
  • Xiao-wen Che
    • 2
  1. 1.Department of Geriatric Medicinethe Affiliated Hospital of Shandong University of Traditional Chinese MedicineJi’nanChina
  2. 2.Department of Respiratory Medicinethe Second Hospital of Shandong UniversityJi’nanChina

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