Skip to main content

Advertisement

SpringerLink
Log in

Effects of Ligustrazine on Airway Inflammation in A Mouse Model of Neutrophilic Asthma

  • Original Article
  • Published:
Chinese Journal of Integrative Medicine Aims and scope Submit manuscript

Abstract

Objective

To investigate the effects of ligustrazine (LTZ) on airway inflammation in a mouse model of neutrophilic asthma (NA).

Methods

Forty healthy C57BL/6 female mice were randomly divided into 4 groups using a random number table, including the normal control, NA, LTZ and dexamethasone (DXM) groups, with 10 rats in each group. The NA mice model was established by the method of ovalbumin combined with lipopolysaccharide sensitization. At 0.5 h before each challenge, LTZ and DXM groups were intraperitoneally injected with LTZ (80 mg/kg) or DXM (0.5 mg/kg) for 14 d, respectively, while the other two groups were given the equal volume of normal saline. After last challenge for 24 h, the aerosol inhalation of methacholine was performed and the airway reactivity was measured. The bronchoalveolar lavage fluid (BALF) was collected. The Wright-Giemsa staining was used for total white blood cells and differential counts. The levels of cytokines interleukin (IL)-17 and IL-10 were detected by enzyme-linked immunosorbent assay. The pathological change of lung tissue was observed by hematoxylin eosin staining.

Results

The airway responsiveness of the NA group was signifificantly higher than the normal control group (P<0.05), while those in the LTZ and DXM groups were signifificantly lower than the NA group (P<0.05). The neutrophil and eosinophil counts in the LTZ and DXM groups were signifificantly lower than the NA group (P<0.05), and those in the LTZ group were signifificantly lower than the DXM group (P<0.05). There were a large number of peribronchiolar and perivascular inflammatory cells in fifiltration in the NA group. The airway inflflammation in the LTZ and DXM groups were signifificantly alleviated than the NA group. The infifiltration in the LTZ group was signifificantly reduced than the DXM group. Compared with the normal control group, the IL-17 level in BALF was signifificantly increased and the IL-10 level in BALF was signifificantly decreased in the NA group (P<0.05). LTZ and DXM treatment signifificantly decreased IL-17 levels and increased IL-10 levels compared with the NA group (P<0.05), and the changes in the above indices were more signifificant in the LTZ group (P<0.05).

Conclusion

LTZ could alleviate the airway inflflammation in the NA mice model through increasing the IL-10 level and decreasing the IL-17 level.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Mcgrath KW, Icitovic N, Boushey HA, Lazarus SC, Sutherland ER, Chinchilli VM, et al. A large subgroup of mild-to-moderate asthma is persistently noneosinophilic. Am J Respir Crit Care Med 2012;185:612–619.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Zhang YX, Nong GM, Liao J, Jiang M, Liang XA, Liu J. Application of induced sputum cytology analysis for childhood asthma subtype. Chin J Appl Clin Pediatr 2010;25:129–132.

    Google Scholar 

  3. Jatakanon A, Uasuf C, Maziak W, Lim S, Chung F, Barnes PJ. Neutrophilic inflammation in severe persistent asthma. Am J Respir Crit Care Med 2012;160:1532–1539.

    Article  Google Scholar 

  4. Zhao Y, Yang J, Gao YD, Guo W. Th17 immunity in patients with allergic asthma. Intern Arch Allergy Immun 2010;151:297–307.

    Article  CAS  Google Scholar 

  5. Zhou C, Yin KS, Chen JH, Shi Y. Changes of the ratio of T helper 17 cells and the level of IL-17 in bronchial asthmatic mice. J Nanjing Med Univ (Nat Sci) 2009;29:109–112.

    Google Scholar 

  6. Zhang XP, Jiang J, Cheng QH, Ye Q, Li WJ, Zhu H, et al. Protective effects of ligustrazine, kakonein and panax notoginsenoside on the small intestine and immune organs of rats with severe acute pancreatitis. Hepatobiliary Pancreatic Dis Int 2011;10:632–637.

    Article  Google Scholar 

  7. Liu RT, Li BZ. Clinical observation of Ligustrazine Injection adjuvant therapy 36 cases infant with bronchial asthma. China Med Herald (Chin) 2011;8:66–68.

    Google Scholar 

  8. Zhu W, Tian H. The treatment of 38 children with asthma in acute stage by tetramethylpyrazine and small dosages of tripterygioside. West J Tradit Chin Med (Chin) 2015;28:102–104.

    Google Scholar 

  9. Xie H, Sun SH. The observation of azithromycin combined ligustrazine on the treatment of glucocorticoid resistance type of asthma. Modern J Integr Tradit Chin West Med (Chin) 2003;13:987–988.

    Google Scholar 

  10. Jiang H, Chen X, Wang JY, Xue YF. Effect of ligustrazine on Th17 cell and balance of Th17/Treg in asthma model mouse. Chin J Immun 2014;10:1339–1343.

    Google Scholar 

  11. Wang YJ, Zhu HZ, Kong XC, Lu L, Liu L, Zhang Y, et al. Observation of the effect of ligustrazine on improving the airway of asthmatic rats. Chin Tradit Patent Med (Chin) 2014;36:834–837.

    CAS  Google Scholar 

  12. Wilson RH, Whitehead GS, Nakano H, Free ME, Kolls JK, Cook DN. Allergic sensitization through the airway primes Th17-dependent neutrophilia and airway hyperresponsiveness. Am J Respir Critic Care Med 2009;180:720–730.

    Article  CAS  Google Scholar 

  13. Liu XW, Jiang M, Nong GM, Liu HY, Peng F. To establish a mice model of neutrophilic asthma and research the airway hyperreactivity. Chin J Asthma (Electronic Version) 2013;7:151–158.

    Google Scholar 

  14. Wei Y, Luo QL, Sun J, Chen MX, Liu F, Dong JC. Bu-Shen-Yi-Qi Formulae suppress chronic airway inflammation and regulate Th17/Treg imbalance in the murine ovalbumin asthma model. J Ethnopharmacol 2015;164:368–377.

    Article  PubMed  Google Scholar 

  15. Mckinley L, Alcorn JF, Peterson A, Dupont RB, Kapadia S, Logar Alision, et al. Th17 cells mediate steroid-resistant airway inflammation and airway hyperresponsiveness in mice. J Immun 2008;181:4089–4097.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. Zhang LQ. Clinical observation of pidotimod and ligustrazine in children with bronchial asthma. Shaanxi Med J (Chin) 2013;42:355–356.

    CAS  Google Scholar 

  17. Che XW, Wang H, Zhang Y, Wang W. Effect of Ligustrazine Injection on levels of interleukin-4 and interferon-γ in patients with bronchial asthma. Chin J Integr Med 2008;14:217–220.

    Article  CAS  PubMed  Google Scholar 

  18. Pelaia G, Vatrella A, Maselli R. The potential of biologics for the treatment of asthma. Nature Rev Drug Discov 2012;11:958–972.

    Article  CAS  Google Scholar 

  19. Qiu YY, Zhang YW, Qian XF, Bian T. MiR-371, miR-138, miR-544, miR-145, and miR-214 could modulate Th1/Th2 balance in asthma through the combinatorial regulation of Runx3. Am J Transl Res 2017;9:3184–3199.

    PubMed  PubMed Central  Google Scholar 

  20. Wang ZE, Zhou XN, Yang Yi, Liu ZY. Effect of Jian'erle Granule on Th17/Treg imbalance of asthma mice. Chin J Integr Med 2016;36:1510–1514.

    Google Scholar 

  21. Henness S, Van TE, Ge Q, Armour CL, Hughes JM, Ammit AJ. IL-17A acts via p38 MAPK to increase stability of TNFalpha-induced IL-8 mRNA in human ASM. Am J Physiol Lung Cell Mol Physiol 2006;290:1283–1290.

    Article  Google Scholar 

  22. Newcomb DC, Jr PR. Th17-mediated inflammation in asthma. Current Opin Immun 2013;25:755–760.

    Article  CAS  Google Scholar 

  23. Eusebio M, Kuna P, Kraszula L, Kupczyk M, Pietruczuk M. The relative values of CD8+CD25+Foxp3 brigh Treg cells correlate with selected lung function parameters in asthma. Int J Immunopath Pharmac 2015;28:218–226.

    Article  CAS  Google Scholar 

  24. Chen CR, Deng JM, Gong C, Zou XY, Wei X, Yang ML, et al. Study on the imbalance of IL-1, IL-10 and IL-4, IFN-γ in peripheral blood of chronic persistent allergic bronchial asthma. J Guangxi Med Univ (Chin) 2014;31:414–415.

    CAS  Google Scholar 

  25. Shi YH, Shi GC, Wan HY, Jiang LH, Ai XY, Zhu HX, et al. The prevalence of blood Thl7 and CD4+CD25+ Treg cells in patients with bronchial asthma. Chin J Immun (Chin) 2010;26:740–743.

    CAS  Google Scholar 

  26. Huber S, Gagliani N, Esplugues E, Jr WO, Huber FJ, Chaudhry A, et al. Th17 cells express interleukin-10 receptor and are controlled by Foxp3–, and Foxp3+, regulatory CD4+, T cells in an interleukin-10-dependent manner. Immunity 2011;34:554–565.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Geriatric Medicine, the Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Ji’nan, 250011, China

    Xiao-ming Liu

  2. Department of Respiratory Medicine, the Second Hospital of Shandong University, Ji’nan, 250033, China

    Yong-bin Wang, Qian Wu, Zhong-rui Bian & Xiao-wen Che

Authors
  1. Xiao-ming Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yong-bin Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Qian Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Zhong-rui Bian
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Xiao-wen Che
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Xiao-wen Che.

Additional information

Supported by the Key Scientific and Technological Project of Shandong Province (No. 2014GSF119012), China

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Liu, Xm., Wang, Yb., Wu, Q. et al. Effects of Ligustrazine on Airway Inflammation in A Mouse Model of Neutrophilic Asthma. Chin. J. Integr. Med. 24, 353–358 (2018). https://doi.org/10.1007/s11655-017-2830-0

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11655-017-2830-0

Keywords

Search

Navigation