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Isolation of antifungal compound from Paeonia suffruticosa and its antifungal mechanism

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Abstract

Objective

To isolate antifungal compound from Paeonia suffruticosa, and to find the antifungal mechanisms by observing the ultrastructural modifications of yeasts in growth phase produced by 1,2,3,4,6-penta-O-galloyl-beta-D-glucose (PGG).

Methods

Peony (Paeonia suffruticosa) root bark (PRB) was separated by solvent extraction and purified by high performance liquid chromatography (HPLC) method using analytical and preparative reversed phase C18 column on the basis of bio-assay method. In order to investigate the antifungal mechanism of PGG, Yeasts were submitted to different concentrations [3 × minimum inhibition concentration (MIC), 0.3 × MIC] for 1 h under constant stirring at 30 °C, and transmission electron microscopy was performed.

Results

Based on the antifungal activity of PRB on Candida glabrata CBS138, the antifungal compound were isolated in ethyl acetate layer of PRB and identified as PGG by mass spectrometry, 1H nuclear magnetic resonance (NMR) analyses, with molecular weight of 940 and molecular formular as C41H32O26. Transmission electron microscopy showed that PGG degraded the cell wall envelope.

Conclusion

The results suggest that PGG may be responsible for the antifungal activity of PRB by disrupting the structure of cell wall directly.

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Authors and Affiliations

  1. Department of Infectious Diseases, Beijing Friendship Hospital, Capital Medical University, Beijing, 100050, China

    Yong Zhao  (赵 勇), Bao-en Wang  (王宝恩), Shu-wen Zhang  (张淑文), Hong Wang  (王 红), Ai-min Ren  (任爱民) & En-tong Yi  (衣恩通)

  2. School of Chemical Biology and Pharmaceutical Sciences, Capital Medical University, Beijing, 100069, China

    Shu-min Yang  (杨淑敏)

Authors
  1. Yong Zhao  (赵 勇)
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  2. Bao-en Wang  (王宝恩)
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  3. Shu-wen Zhang  (张淑文)
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  4. Shu-min Yang  (杨淑敏)
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  5. Hong Wang  (王 红)
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  6. Ai-min Ren  (任爱民)
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  7. En-tong Yi  (衣恩通)
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Correspondence to Bao-en Wang  (王宝恩).

Additional information

Supported in part by a grant from Bureau of Personnel of Beijing (No. 100005)

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Zhao, Y., Wang, Be., Zhang, Sw. et al. Isolation of antifungal compound from Paeonia suffruticosa and its antifungal mechanism. Chin. J. Integr. Med. 21, 211–216 (2015). https://doi.org/10.1007/s11655-014-1805-7

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  • DOI: https://doi.org/10.1007/s11655-014-1805-7

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