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Zhuanggu Jianxi Decoction (壮骨健膝方) limits interleukin-1 β-induced degeneration chondrocytes via the caveolin-p38 MAPK signal pathway

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Abstract

Objective

To evaluate the effect of Zhuanggu Jianxi Decoction (壮骨健膝方, ZGJXD) on interleukin-1 β (IL-1 β)-induced degeneration of chondrocytes (CDs) as well as the activation of caveolin-p38 mitogen-activated protein kinase (MAPK) signal pathway, investigating the possible molecular mechanism that ZGJXD treats osteoarthritis.

Methods

Serum pharmacology was applied in the present study, where ZGJXD was orally administrated to New Zealand rabbits and then ZGJXD containing serum (ZGJXD-S) was collected for following in vitro experiments. CDs were isolated aseptically from New Zealand rabbits and then cultured in vitro. Upon IL-1 β stimulation, the degeneration of CDs was verified by inverted microscope, toluidine blue stain and type II collagen immunocytochemistry. After IL-1 β-stimulated CDs were intervened with blank control serum, ZGJXD-S, together with or without SB203580 (a specific inhibitor of p38 MAPK) for 48 h, caveolin-1 protein expression and the phosphorylation level of p38 were determined by Western blotting, and the mRNA expression of IL-1 β, tumor necrosis factor α (TNF-α), matrix metalloproteinase 3 (MMP-3) and MMP-13 were examined by real-time polymerase chain reaction.

Results

IL-1 β stimulation induced degeneration of CDs, increased caveolin-1 expression and p38 phosphorylation, up-regulated the mRNA level of IL-1 β, TNF-α, MMP-3 and MMP-13. However, the IL-1 β-induced activation of caveolin-p38 signaling and alteration in the expression of p38 downstream target genes were suppressed by ZGJXD-S and/or SB203580 in CDs.

Conclusion

ZGJXD can prevent CDs degeneration via inhibition of caveolin-p38 MAPK signal pathway, which might be one of the mechanisms that ZGJXD treats osteoarthritis.

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Authors and Affiliations

  1. Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China

    Hu Yan  (闫 虎), You-xin Su  (苏友新), Xue-yi Lin  (林学义), Bao-jun Chen  (陈宝军), Qing Zhang  (张 庆), Zi-yi Zhang  (张子怡), Yi-ru Wang  (王艺茹), Ya-nan Li  (李亚楠), Mei-li Lu  (卢美丽), Zhen He  (何 浈), Lu Sheng  (盛 录) & Wen-ting Wang  (王雯婷)

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  1. Hu Yan  (闫 虎)
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  2. You-xin Su  (苏友新)
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  3. Xue-yi Lin  (林学义)
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  4. Bao-jun Chen  (陈宝军)
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  5. Qing Zhang  (张 庆)
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  6. Zi-yi Zhang  (张子怡)
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  7. Yi-ru Wang  (王艺茹)
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  8. Ya-nan Li  (李亚楠)
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  9. Mei-li Lu  (卢美丽)
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  10. Zhen He  (何 浈)
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  11. Lu Sheng  (盛 录)
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  12. Wen-ting Wang  (王雯婷)
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Correspondence to You-xin Su  (苏友新).

Additional information

Supported by the National Natural Science Foundation of China (No. 81072828)

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Yan, H., Su, Yx., Lin, Xy. et al. Zhuanggu Jianxi Decoction (壮骨健膝方) limits interleukin-1 β-induced degeneration chondrocytes via the caveolin-p38 MAPK signal pathway. Chin. J. Integr. Med. 20, 353–359 (2014). https://doi.org/10.1007/s11655-014-1801-y

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  • DOI: https://doi.org/10.1007/s11655-014-1801-y

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