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Therapeutic effect of dimethyl dimethoxy biphenyl dicarboxylate on collagen-induced arthritis in rats

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Abstract

Objective

To study the effect of oral administration of dimethyl dimethoxy biphenyl dicarboxylate (DDB) on adjusting angiogeneic/inflflammatory mediators and ameliorating the pathology of bones in rats with collagen-induced arthritis (CIA).

Methods

Wistar rat model of CIA was set up using bovine collagen type II. Fifty rats were divided into fifive groups randomly: normal, CIA model, DDB treatment, methotrexate (MTX) treatment, and combined DDB+MTX treatment. Ankle joints of rats were imaged with digital X-ray machine to show the destruction of joints. Fore and hind paw and knee joints were removed above the ankle joint then processed for haematoxylin and eosin staining. Plasma levels of vascular endothelial growth factor (VEGF), platelet derived growth factor, interleukin-8 (IL-8), IL-4, tumor necrosis factor α (TNF-α), and cyclooxygenase-2 (COX-2) were quantifified by enzyme-linked immunosorbent assay. Nitric oxide levels were detected by Griess reagent.

Results

Compared with the CIA model group, a remarkable reduction in various angiogenic (VEGF and IL-8) and inflflammatory mediators (TNF-α, IL-4 and COX-2) after treatment with DDB either alone or combined with MTX P<0.05 or P<0.01). Histopathological and X-ray fifindings were confifirmatory to the observed DDB anti-arthritic effect. The DDB-treated group showed amelioration in signs of arthritis which appeared essentially similar to normal.

Conclusion

Our data shed light on the therapeutic effificacy of DDB in experimental rheumatoid arthritis (RA) compared with a choice drug (MTX) and it may be offered as a second-line drug in the treatment of RA.

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Authors and Affiliations

  1. Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute, Menofia University, Sadat Branch, Sadat, 32897, Egypt

    Roba M. Talaat, Amira S. Abo-El-Atta & Sabah M. Farou

  2. Pathology Department, Faculty of Medicine, Tanta University, Tanta, 31111, Egypt

    Karima I. El-Dosoky

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  1. Roba M. Talaat
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  2. Amira S. Abo-El-Atta
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  3. Sabah M. Farou
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  4. Karima I. El-Dosoky
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Correspondence to Roba M. Talaat.

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Talaat, R.M., Abo-El-Atta, A.S., Farou, S.M. et al. Therapeutic effect of dimethyl dimethoxy biphenyl dicarboxylate on collagen-induced arthritis in rats. Chin. J. Integr. Med. 21, 846–854 (2015). https://doi.org/10.1007/s11655-014-1746-1

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