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Relaxant effects of Aike Mixture (艾可合剂) on isolated bladder and prostatic urethral smooth muscle of rabbits

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Abstract

Objective

To observe the relaxant effect of Aike Mixture (艾可合剂, AKM) on isolated bladder and prostatic urethral smooth muscle of rabbits.

Methods

The isolated bladder and prostatic urethral smooth muscle from male rabbits were placed in a Magnus bath and smooth muscle contraction was measured using a biological signal acquisition and analysis system. The effects of AKM in combination with methoxyamine, carbachol and CaCl2 on the contractile tension of muscle strips were determined by cumulative dosing.

Results

AKM dose-dependently reduced contractile tension of bladder trigone smooth muscle (r=0.831, P<0.05), reduced contractile wave amplitude (r=0.837, P<0.05) and decreased contractile frequency (r=−0.917, P<0.01). AKM significantly inhibited the increases in smooth muscle contraction induced by methoxyamine, carbachol and CaCl2.

Conclusion

AKM dose-dependently inhibited the contraction of rabbit isolated bladder and prostatic urethral smooth muscle by antagonizing α1-adrenergic receptors and M-cholinergic receptors.

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Authors and Affiliations

  1. People’s Hospital Affiliated to Fujian University of Traditional Chinese Medicine, Fuzhou, 350004, China

    Min-jian Zhang  (张敏建), Ya-lei Shi  (史亚磊), Wan-jun Cheng  (程宛钧), Zhen-wei Li  (李振伟) & Xiang Lin  (林 湘)

Authors
  1. Min-jian Zhang  (张敏建)
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  2. Ya-lei Shi  (史亚磊)
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  3. Wan-jun Cheng  (程宛钧)
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  4. Zhen-wei Li  (李振伟)
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  5. Xiang Lin  (林 湘)
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Corresponding author

Correspondence to Min-jian Zhang  (张敏建).

Additional information

Supported by the Natural Science Foundation of Fujian Province (No. 2008J0092)

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Zhang, Mj., Shi, Yl., Cheng, Wj. et al. Relaxant effects of Aike Mixture (艾可合剂) on isolated bladder and prostatic urethral smooth muscle of rabbits. Chin. J. Integr. Med. 20, 420–424 (2014). https://doi.org/10.1007/s11655-012-1088-9

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  • DOI: https://doi.org/10.1007/s11655-012-1088-9

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