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The influence of the total flavonoids of Hedysarum polybotry on the proliferation, cell cycle, and expressions of p21Ras and proliferating cell nuclear antigen gene in erythroleukemia cell line K562

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Abstract

Objective

To investigate the effect of total flavonoids of Hedysarum polybotry on the proliferation, cell cycle, and expressions of p21Ras and proliferating cell nuclear antigen (PCNA) gene in erythroleukemia cell line K562.

Methods

The effect of total flavonoids of Hedysarum polybotry on K562 cell line survival was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) reduction assay. The time- and dose-dependent manner was also observed. The cell cycle and apoptosis were analyzed with flow cytometry (FCM). The immunocytochemistry method was applied to quantitatively analyze the effects of flavonoids of Hedysarum polybotry on changes p21Ras and PCNA gene expressions.

Results

Flavonoids of Hedysarum polybotry (20–100 μg/mL) significantly inhibited the proliferation of K562 cells in a time- and dose-dependent manner. After K562 cells were cultured for 48 h, total flavonoids of Hedysarum polybotry had no significant effect on the apoptosis of K562 cells but showed significantly inhibition (P<0.01), indicating that total flavonoids of Hedysarum polybotry could induce K562 cells arrested at G0/G1 and G2/M phases. Compared with the control group, p21Ras and PCNA gene expressions were decreased significantly in K562 cells treated with total flavonoids of Hedysarum polybotry (40 and 80 μg/mL, respectively) for 48 h.

Conclusion

The inhibitory effect on proliferation of K562 cells was observed in the groups treated with flavonoids of Hedysarum polybotry, which might be related to cells arresting.

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Authors and Affiliations

  1. Gansu College of Traditional Chinese Medicine, Lanzhou, 730000, China

    Ya-li Wang  (王雅莉), Ya-li Luo  (骆亚莉), Che Chen  (陈 彻), Neng-lian Li  (李能莲), Ya-li She  (舍雅莉) & Li Zhang  (张 立)

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  1. Ya-li Wang  (王雅莉)
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  2. Ya-li Luo  (骆亚莉)
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  3. Che Chen  (陈 彻)
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  4. Neng-lian Li  (李能莲)
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  5. Ya-li She  (舍雅莉)
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  6. Li Zhang  (张 立)
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Correspondence to Ya-li Wang  (王雅莉).

Additional information

Supported by the Natural Science Foundation for Middle-Aged and Young Scientist of Gansu Province (No. YS031-A21-015)

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Wang, Yl., Luo, Yl., Chen, C. et al. The influence of the total flavonoids of Hedysarum polybotry on the proliferation, cell cycle, and expressions of p21Ras and proliferating cell nuclear antigen gene in erythroleukemia cell line K562. Chin. J. Integr. Med. 18, 385–390 (2012). https://doi.org/10.1007/s11655-011-0952-3

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  • DOI: https://doi.org/10.1007/s11655-011-0952-3

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