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Pure total flavonoids from Citrus paradisi Macfad induce leukemia cell apoptosis in vitro

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Abstract

Objective

To investigate the potential effect of pure total flavonoids from Citrus paradisi Macfad peel (PTFC) on the proliferation of human myeloid leukemia cells Kasumi-1, HL-60 and K562, and the underlying mechanisms.

Methods

PTFC was extracted from Citrus paradisi Macfad peel and was identified by high performance liquid chromatography. The effect of PTFC on the proliferation and apoptosis of leukemia cells were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, fluorescent microscopy and flow cytometry, respectively. The effect of PTFC on the expression levels of apoptosis-related regulators was determined by Western blot assay.

Results

Treatment with PTFC inhibited leukemia cell proliferation in a dose- and time-dependent manner and triggered Kasumi-1 cell apoptosis. Treatment with PTFC significantly increased the levels of activated poly adenosine diphosphate-ribosepolymerase and caspase-3/-9, but reduced the levels of Mcl-1 expression in Kasumi-1 cells. However, PTFC did not obviously induce HL-60 cell apoptosis.

Conclusion

PTFC inhibited leukemia cell proliferation and induced their apoptosis by modulating apoptosisrelated regulator expression in leukemia cells in vitro.

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Authors and Affiliations

  1. Department of Hematology, the First Hospital Affiliated to Zhejiang University of Traditional Chinese Medicine, Hangzhou, 310006, China

    Tie-ying Dai  (戴铁颖), Bo Wang  (王 博), Sheng-yun Lin  (林圣云), Jian-ping Jiang  (蒋剑平) & Li-qiang Wu  (武利强)

  2. Department of Hematology, the First Affiliated Hospital of Medical School of Zhejiang University, Hangzhou, 310003, China

    Wen-bing Qian  (钱文斌)

Authors
  1. Tie-ying Dai  (戴铁颖)
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  2. Bo Wang  (王 博)
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  3. Sheng-yun Lin  (林圣云)
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  4. Jian-ping Jiang  (蒋剑平)
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  5. Li-qiang Wu  (武利强)
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  6. Wen-bing Qian  (钱文斌)
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Corresponding author

Correspondence to Sheng-yun Lin  (林圣云).

Additional information

Supported by Zhejiang Provincial Natural Science Foundation of China (No. LY14H080003)

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Dai, Ty., Wang, B., Lin, Sy. et al. Pure total flavonoids from Citrus paradisi Macfad induce leukemia cell apoptosis in vitro . Chin. J. Integr. Med. 23, 370–375 (2017). https://doi.org/10.1007/s11655-016-2593-z

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  • DOI: https://doi.org/10.1007/s11655-016-2593-z

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