Abstract
Objective
To observe the effect of Quyu Jiedu Granules (祛瘀解毒颗粒, QJG) on the micro- microenvironment of ova in patients with endometriosis (EM). environment
Methods
Twenty EM patients who received in vitro fertilization and embryo transfer (IVF-ET) were randomized equally into a treated group and a control group. Further, 20 patients who received IVF-ET due to oviduct factors were enrolled into a non-endometriosis group. The dosage of gonadotrophic hormone used, the number of ova attained, fertilization rate and clinical pregnancy rate were all observed, and the levels of tumor necrosis factor α (TNF-⇌) and interleukin 6 (IL-6) in follicular fluid as well as their mRNA expressions in ovarian granular cells were detected by RT-PCR on the very day of ovum attainment.
Results
The ova attainment (13.80±6.87) and fertilization rate (0.69±0.31) in the treated group were all higher than the corresponding values in the control group (9.80±5.32 and 0.47±0.22); the follicular fluid contents of TNF-α and IL-6 in the treated group were 1.38±0.21 ng/mL and 130.56±12.81 pg/mL, respectively, which were lower than those in the control group (1.98±0.34 ng/mL and 146.83±17.65 pg/mL, respectively). Further, the treated group showed much lower mRNA expressions of TNF-α and IL-6 in ovarian granular cells.
Conclusions
The elevation of TNF-α and IL-6 contents in follicular fluid and their mRNA expressions in ovarian granular cells are possibly related to the low quality of ova in EM; QJG might raise the ova quality by reducing TNF-α and IL-6 levels to improve the living micro-environment for the ova.
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References
Gazvani R, Templeton A. New considetrations for the pathothogenesis of endometriosis. Int J Gynaecol Obstet 2002;76:117–126.
Lang JH, ed. Basic and clinical study of endometriosis. Beijing: Chinese Academy of Medical Sciences and Peking Union Medical College Press; 2003:1–6.
Pellicer A, Albert C, Garrido N, et al. The pathophysiology of endometriosis associated infertility: follicular enviroment and embryo quality. J Reprod Fertil Suppl 2000;55:109–119.
Special Committee of Gynecology and Obstetrics, Chinese Association of Integrative Medicine. Standard of diagnosis and treatment of endometriosis, pregnancy-induced hypertension and female infertility. Chin J Integr Med (Chin) 1991;11:376
Yue J, ed. Obstetrics and gynecology. 6th ed. Bejing: People’s Medical Publishing House, 2004:381–384.
Luo YK, ed. Practical gynecology of traditional Chinese medicine. Shanghai: Shanghai Scientific & Technical Publishers, 1996: 278.
Wei HM, Tian ZG, Liu J. The set-up and preliminary clinical application of the genetic testing method of 14 cytokines. Chin J Microbiol Immunol (Chin) 2002; 20: 180–181.
Mahutte NG, Arici A. New advances in the understanding of endometriosis related infertility. J Reprod Immunol 2002;55:73–83.
Harada T, Yoshioka H, Yoshida S, Iwabe T, Onohara Y, Tanikawa M, et al. Increased interleukin-6 levels in peritoneal fluid of infertile patients with active endometriosis. Am J Obstet Gynecol 1997;176:593–597.
Ghersevich S, Isomaa V, Vihko P. Cytokine regulation of the expression of estrogenic biosynthetic enzymes in cultured rat granulosa cells. Mol Cell Endocrinol 2001;172:21–30.
Specialty Committee of Obstetrics and Gynecology, Chinese Association of Integrative Medicine. Standard for diagnosis and treatment of endometriosis, syndrome of hypertension of pregnancy and female infertility by integrative medicine. Chin J Integr Tradit West Med (Chin)1991;11:376.
Lian F, Wang XB, Zhang JW, Zhang N, Sun ZG, Li HX. Thinking and methods of TCM treatment endometriosis. J Tradit Chin Med (Chin) 2002;43(7):545–546.
Carlberg M, Nejaty J, Fröysa B, Guan Y, Söder O, Bergqvist A. Elevated expression of tumor necrosis factor alpha in cultured granulosa cells from women with endometriosis. Hum Reprod 2000;15:1250–1255.
Naz RK, Zhu X, Menge AC. Expression of tumor necrosis factor-alpha and its receptors type I and type II in human oocytes. Mol Reprod Dev 1997;47:127–133.
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Supported by the National Natural Science Foundation of China (No. 30672706)
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Lian, F., Li, Xl., Sun, Zg. et al. Effect of Quyu Jiedu Granule (祛瘀解毒颗粒) on microenvironment of ova in patients with endometriosis. Chin. J. Integr. Med. 15, 42–46 (2009). https://doi.org/10.1007/s11655-009-0042-8
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DOI: https://doi.org/10.1007/s11655-009-0042-8