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Effect of Huanglian Jiedu Decoction (黄连解毒汤) on glucose transporter 4 expression in adipose and skeletal muscle tissues of insulin resistant rats

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Abstract

Objective

To investigate the effects of Huanglian Jiedu Decoction (黄连解毒汤, HLJDD) on glucose transporter 4 (GLUT4) protein expressions in insulin-resistant murine target tissues.

Methods

The experimental male Wistar rats were established into insulin resistant models by injecting streptozotocin (STZ 30 mg/kg) via caudal vein and feeding them with high fat high caloric diet, and randomly divided into the model group, the aspirin group and the HLJDD group. Besides, a normal group was set up for control. Changes of body weight (BW), levels of serum fasting blood glucose (FBG), serum fasting insulin (FINS) and oral glucose tolerance test (OGTT) were routinely determined. The expression of GLUT4 protein in adipose and skeletal muscle tissues before and after insulin stimulation was determined with Western blot.

Results

In the HLJDD group after treatment, BW and FBG got decreased, OGTT improved, and the expression and translocation of GLUT4 protein elevated obviously, either before or after insulin stimulation, as compared with those in the model group, showing significant differences respectively.

Conclusion

The mechanism of improving insulin resistance by HLJDD is probably associated with its effect in elevating GLUT4 protein expression and translocation in adipose and skeletal muscle tissues of insulin resistant rats.

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Authors and Affiliations

  1. Institute of Integrative Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China

    Chen Guang  (陈 广), Lu Fu-er  (陆 付耳), Jin Dan  (金丹), Xu Li-jun  (徐丽君) & Wang Kai-fu  (王开富)

Authors
  1. Chen Guang  (陈 广)
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  2. Lu Fu-er  (陆 付耳)
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  3. Jin Dan  (金丹)
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  4. Xu Li-jun  (徐丽君)
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  5. Wang Kai-fu  (王开富)
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Corresponding author

Correspondence to Lu Fu-er  (陆 付耳).

Additional information

Supported by the National Natural Science Foundation of P. R. China (No. 30371816) and Specialized Research Fund for the Doctoral Program of Higher Education (No. 20030487008)

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Chen, G., Lu, Fe., Jin, D. et al. Effect of Huanglian Jiedu Decoction (黄连解毒汤) on glucose transporter 4 expression in adipose and skeletal muscle tissues of insulin resistant rats. Chin. J Integ. Med. 13, 41–45 (2007). https://doi.org/10.1007/s11655-007-0041-9

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  • DOI: https://doi.org/10.1007/s11655-007-0041-9

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