Abstract
A description of a successful direct somatic embryogenesis induction from immature zygotic embryos of a camphor tree (Cinnamomum camphora L.) is presented. After a subculture of 2–3 years, embryogenic calli could be derived from primary somatic embryos. Immature zygotic embryos were cultured on a Murashige and Skoog (MS) basal medium supplemented with a range of combinations of cytokinins (BA) and auxins (2,4-D or NAA) for somatic embryo induction. Primary somatic embryos could be induced directly in almost all PGR combinations. A positive effect of 2,4-D on somatic embryogenesis from immature zygotic embryos of camphor tree was obtained. BA at appropriate concentrations (< 5 mg·L−1) had an effect similar to 2,4-D, whereas high concentrations (> 5 mg·L−1) of BA had the effect of restraining somatic embryo induction. NAA had a less positive effect on somatic embryogenesis than 2,4-D.
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Alemanno L, Berthouly M, Michaux-Ferriere N. 1996. Histology of somatic embryogenesis from floral tissues of cocoa. Plant Cell Tiss. Org. Cult., 46: 187–194
Canhoto J M, Lopes M L, Cruz G S. 1999. Somatic embryogenesis and plant regeneration in myrtle (Myrtaceae). Plant Cell Tiss. Org. Cult., 57: 13–21
Carimi F, Lorenzo R D, Crescimanno F G. 1997. Callus induction and somatic embryogensis in carob (Ceratonia siliqua L.) from ovule culture. Sci. Hort., 70: 73–79
Chen D L. 1998. Study on Darna trima (Moore), a new pest of Cinnamomum camphora. Entomol. J. East China, 7(2): 45–47
Das D K, Teddy M K, Upadhyaya K C, Sopory S K. 2002. An efficient leaf-disc culture method for the regeneration via somatic embryogenesis and transformation of grape (Vitis vinifera L.) Plant Cell Rep., 20: 999–1005
Garin E, Grenier E, March G. 1997. Somatic embryogenesis in wild cherry (Prunus avium). Plant Cell Tiss. Org. Cult., 48: 83–91
Hu Z, Yang J, Guo G Q, Zheng G C. 2002. High-efficiency transformation of Licium barbarum mediated by Agrobacterium tumefaciens and transgenic plant regeneration via somatic embryogenesis. Plant Cell Rep., 21: 233–237
Huong L T L, Baicco M, Huy B P, Mezzetti B, Santilocchi R, Rosati P. 1999. Somatic embryogenesis in Canary Island date palm. Plant Cell Tiss. Org. Cult., 56: 1–7
Igasaki T, Sato T, Akashi N, Mohri T, Maruyama E, Kinoshita I, Walter C, Shinohara K. 2003. Somatic embryogenesis and plant regeneration from immature zygotic embryos of Cryptomeria japonica D.Don. Plant Cell Rep., 22:239–243
Kim Y W, Youn Y, Noh E R, Kim J C. 1999. Somatic embryogenesis and plant regeneration from immature zygotic embryos of Japanese larch (Larix leptolepis). Plant Cell Tiss. Org. Cult., 55: 95–101
Kiss J, Heszky L E, Kiss E, Gyulai G. 1992. High frequency adventive embryogenesis on somatic embryos of anther, filament and immature proembryo origin in horse-chestnut (Aesculus hippocastanum L.). Plant Cell Tiss. Org. Cult., 30: 59–64
Kumria R, Waie B, Rajam M V. 2001. Plant regeneration from transformed embryogenic callus of an elite Indica rice via Agrobacterium. Plant Cell Tiss. Org. Cult., 67: 63–71
Lelu M A P, Bornman C H. 1990. Induction of somatic embryogenesis in excised cotyledons of Picea glauca and Picea mariana. Plant Physiol. Biochem., 28: 785–791
Liu X Y. 1997. Occurrence and control termites in ancient and valuable trees in Guangzhou. Nat. Enem. Insects, 18(4): 169–172 (in Chinese with an English abstract)
Merkle S A, Neu K A, Battle P J, Bailey R L. 1998. Somatic embryogenesis from immature and mature tissues of sweetgum (Liquidambar styraciflua). Plant Sci., 132: 169–178
Merkle S A, Battle P J. 2000. Enhancement of embryogenic culture initiation from tissues of mature sweetgum trees, Plant Cell Rep., 19: 268–273
Mondal T, Bhattacharya A, Ahuja P, Chand P. 2001. Transgenic tea [Camellia sinensis (L.) O. Kuntze cv. Kangra Jat] plants obtained by Agrobacterium-mediated transformation of somatic embryos. Plant Cell Rep., 20: 712–720
Murashige T, Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture, Physiol. Plant., 15, 473–497
de Sousa Araújo S, Ana Sofia Roldão Lopes Amaral Duque Dulce Maria Metelo Fernandes dos Santos Manuel Pedro Salema Fevereiro. 2004. An efficient transformation method to regenerate a high number of transgenic plants using a new embryogenic line of Medicago truncatula cv. Jemalong. Plant Cell Tiss. Org. Cult., 78: 123–131
Shuerman P L, Dandekar A M. 1993. Transformation of temperate woody crops: progress and potentials. Sci. Hort., 55: 101–124
Sullivan J, Lagrimoni L M. 1993. Transformation of Liquidambar styraciflua using Agrobacterium tumefaciens. Plant Cell Rep., 12: 303–306
Tang W. 2000. High-frequency plant regeneration via somatic embryogenesis and organogenesis and in vitro flowering of regenerated plantlets in Panax ginseng. Plant. Cell Rep., 19: 727–732
Te-chato S, Rungnoi O. 2000. Induction of somatic embryogenesis from leaves of Sadao Chang (Azadirachta excelsa (Jack) Jacobs). Sci. Hort., 86: 311–321
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Du, L., Zhou, S. & Bao, Mz. Effect of plant growth regulators on direct somatic embryogenesis in camphor tree (Cinnamomum camphora L.) from immature zygotic embryos and embryogenic calli induction. For. Stud. China 9, 267–271 (2007). https://doi.org/10.1007/s11632-007-0042-y
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DOI: https://doi.org/10.1007/s11632-007-0042-y