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Constitutive expression of sense & antisense PtAP3, an AP3 homologue gene of Populus tomentosa, affects growth and flowering time in transgenic tobacco

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Forestry Studies in China

Abstract

To analyze the function of PtAP3, an APETALA3 (AP3) homologue gene isolated from Populus tomentosa Carr., the full length sequence (1 797 bp) and a fragment (870 bp) of PtAP3 were fused to a CaMV 35S promoter of pBI121 to generate the sense and antisense constructs of PtAP3. These constructs were transformed into tobacco by Agrobacterium infection of leaf disks and selection on kanamycin medium. Some sense and antisense transgenic tobacco plants were obtained by PCR and Southern blot analysis. Great phenotypic differences in transgenic tobacco plants were observed. Almost all of sense PtAP3 to transgenic tobaccos showed a higher growth rate than those of antisense transformants and a few developed pregnancy earlier than wild type seedlings and antisense transformants under the same conditions.

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Authors and Affiliations

  1. Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, 100083, Beijing, P. R. China

    Wang Dong-mei, An Xin-min & Zhang Zhi-yi

Authors
  1. Wang Dong-mei
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  2. An Xin-min
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  3. Zhang Zhi-yi
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Correspondence to Zhang Zhi-yi.

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[Supported by the National Natural Science Foundation of China (Grant No. 30371175) and Postdoctoral Foundation of China (Grant No. 2002032041)]

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Wang, Dm., An, Xm. & Zhang, Zy. Constitutive expression of sense & antisense PtAP3, an AP3 homologue gene of Populus tomentosa, affects growth and flowering time in transgenic tobacco. For. Stud. China 7, 22–27 (2005). https://doi.org/10.1007/s11632-005-0026-8

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  • DOI: https://doi.org/10.1007/s11632-005-0026-8

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