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Identification of CpTI gene integration for 2-year-old transgenic poplars at DNA level

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Forestry Studies in China

Abstract

The putative transgenic hybrid triploid poplars [(P. tomentosa × P. bolleana) × P. tomentosa] with CpTI gene have been outplanted in test field for 2 years. Although the authors’ previous studies have proved that they are highly resistant to 3 species of poplar-threatening insect pests and contain high content of CpTI protein in foliage, incorporation status of foreign CpTI gene in poplar genome is uncertain. In this present study, the incorporation of foreign CpTI gene in genome of 5 transgenic poplars was confirmed by PCR and Southern blotting analysis. DNA amplification showed that there were clear DNA bands of about 450bp specific to CpTI gene in transgenic lanes, while no corresponding band in non-transgenic lane was observed. Correspondingly, clear DNA hybridization signals and no signal were exhibited on film for DNA Southern blotting analysis in transgenic lanes and non-transgenic lane, respectively, which further confirmed the stable integration of foreign CpTI gene in genome of 2-year-old transgenic poplar.

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Authors and Affiliations

  1. College of Biological Sciences and Biotechnology, Beijing Forestry University, 100083, Beijing, P. R. China

    Zhang Qian, Lin Shanzhi, Lin Yuanzhen, Zhang Zhiyi, Zou Yening & Wang Zeliang

  2. College of Resources and Environment, Beijing Forestry University, 100083, Beijing, P. R. China

    Liu Haijun

Authors
  1. Zhang Qian
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  2. Lin Shanzhi
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  3. Lin Yuanzhen
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  4. Zhang Zhiyi
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  5. Liu Haijun
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  6. Zou Yening
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  7. Wang Zeliang
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Correspondence to Zhang Zhiyi.

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[Supported by the National Project in Transgenic Plant and Application (Grant No. J2002-B003)]

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Zhang, Q., Lin, S., Lin, Y. et al. Identification of CpTI gene integration for 2-year-old transgenic poplars at DNA level. For. Stud. China 6, 15–19 (2004). https://doi.org/10.1007/s11632-004-0035-z

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  • DOI: https://doi.org/10.1007/s11632-004-0035-z

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