Abstract
To assess the effects of single and combined pollution of cadmium (Cd) and mercury (Hg) on soil microbial community structural and functional diversities, an incubation experiment was conducted, by employing two soils, namely, the marine sediment silty loam soil and the yellowish-red soil, in which five levels of Cd, Hg and Cd and Hg in combination were added. After being incubated for 56 days, the phospholipid fatty acids (PLFAs) profile and sole carbon source utilization pattern (BIOLOG) of the samples were tested. The results showed that the composition of the microbial communities changed significantly at different levels of metals application. The principal component analyses (PCA) of PLFAs indicated that the structure of the microbial community was also significantly altered with increasing levels of metals, with increasing PLFAs biomarkers for fungi and actinomycetes, and increasing ratio of Gram-positive to Gram-negative bacteria. Sole carbon source utilization pattern analysis revealed that single and combined application of Cd and Hg inhibited significantly the functional activity of soil microorganisms, the functional diversity indices [Richness (S), Shannon-Wiener indices (H) and Evenness (E H )] were significantly lower in polluted soils than those in non-polluted soils, which also significantly altered with increasing levels of metals. PCA for the sole carbon source utilization pattern also indicated that the metal contamination could result in a variable soil microbial community. The results revealed that the combination of Cd and Hg had higher toxicity to soil microbial community structural and functional diversities than the individual application of Cd or Hg.
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References
ACSSSC (Agrochemistry Commission, Soil Science Society of China, ed.) (1983) Routine Methods for Soil and Agrochemical Analysis [M]. pp.457. Science Press, Beijing.
Avidano L., Gamalero E., Cossa G..P., and Carraro E. (2005) Characterization of soil health in an Italian polluted site by using microorganisms as bioindicators [J]. Applied Soil Ecology. 30, 21–33.
Bligh E.G.. and Dyer W.J. (1959) A rapid method of total lipid extraction and purification [J]. Canadian Journal of Biochemistry and Physiology. 37, 911–917.
Cai Xinde, Qiu Rongliang, Chen Guizhu, Zeng Xiaowen, and Fang Xiaohang (2006) Response of microbial communities to phytoremediation of nickel contaminated soil [J]. Acta Pedologica Sinica. 43, 919–925 (in Chinese).
Campbell C.D., Grayston S.J., and Hirst D.J. (1997) Use of rhizosphere carbon sources in sole carbon source tests to discriminate soil microbial communities [J]. Journal of Microbiological Methods. 30, 33–41.
Doelman P., Jansen E., Michels M., and Van Til M. (1994) Effects of heavy metals in soil on microbial diversity and activity as shown by the sensitivity-resistance index, an ecologically relevant parameter [J]. Biology and Fertility of Soils. 17, 177–184.
Federle T.W. (1986) Microbial distribution in soil-new techniques. In Perspective in Microbial Ecology (eds. Megusar F. and Gantar M.) [M]. pp.493–498. Slovene Society for Microbiology, Ljubljana.
Frostegård A., Tunlid A., and Bååth E. (1993) Phospholipid fatty acid composition, biomass, and activity of microbial communities from two soil types experimentally exposed to different heavy metals [J]. Applied and Environmental Microbiology. 59, 3605–3617.
Frostegård A., Tunlid A., and Bååth E. (1996) Changes in soil microbial community structure during long-term incubation in two soils experimentally contaminated with metals [J]. Soil Biology and Biochemistry. 28, 55–63.
Frey B., Stemmer M., Widmer F., Luster J., and Sperisen C. (2006) Microbial activity and community structure of a soil after heavy metal contamination in a model forest ecosystem [J]. Soil Biology and Biochemistry. 38, 1745–1756.
Garland J.L. (1996) Patterns of potential C source utilization by rhizosphere communities [J]. Soil Biology and Biochemistry. 28, 223–230.
Harch B.D., Correll R.L., Meech W., Kirkby C.A., and Pankhurst C.E. (1997) Using the Gini coefficient with BIOLOG substrate utilization data to provide an alternative quantitative measure for comparing bacterial soil communities [J]. Journal of Microbiological Methods. 30, 91–101.
Hu Qing, Qi Hongyan, Zeng Jinghai, and Zhang Hongxun (2007) Bacterial diversity in soils around a lead and zinc mine [J]. Journal of Environmental Sciences. 19, 74–79.
Kamala S.K. and Krishnamoorthy R. (2006) Isolation of mercury resistant bacteria and influence of abiotic factors on bioavailability of mercury—A case study in Pulicat Lake North of Chennai, South East India [J]. Science of the Total Environment. 367, 341–353.
Kelly J.J. and Tate R.L.III. (1998) Effects of heavy metal contamination and remediation on soil microbial communities in the vicinity of a zinc smelter [J]. Journal of Environmental Quality. 27, 609–617.
Kelly J.J., HaÈggblom M., and Tate R.L.III. (1999) Changes in soil microbial communities over time resulting from one time application of zinc: a laboratory microcosm study [J]. Soil Biology and Biochemistry. 31, 1455–1456.
Khan S., Cao Qin, Abd Hesham El-Latif, Xia Yue, and He Jizheng (2007) Soil enzymatic activities and microbial community structure with different application rates of Cd and Pb [J]. Journal of Environmental Sciences. 19, 834–840.
King S.A., Behnke S., Slack K., Krabbenhoft D.P., Nordstrom D.K., Burr M.D., and Striegl R.G.. (2006) Mercury in water and biomass of microbial communities in hot springs of Yellowstone National Park, USA [J]. Applied Geochemistry. 21, 1868–1879.
Larkin R.P. (2003) Characterization of soil microbial communities under different potato cropping systems by microbial population dynamics, substrate utilization, and fatty acid profiles [J]. Soil Biology and Biochemistry. 35, 1451–1466.
Lazzaro A., Schulin R., Widmer F., and Frey B. (2006) Changes in lead availability affect bacterial community structure but not basal respiration in a microcosm study with forest soils [J]. Science of the Total Environment. 371, 110–124.
Liao Min, Chen Chengli, and Huang Changyong (2005) Effect of heavy metals on soil microbial activity and diversity in a reclaimed mining wasteland of red soil area [J]. Journal of Environmental Sciences—China. 17, 832–837.
Liao Min and Xie Xiaomei (2007) Effect of heavy metals on substrate utilization pattern, biomass and activity of microbial communities in a reclaimed mining wasteland of red soil area [J]. Ecotoxicology and Environmental Safety. 66, 217–223.
O’Leary W.M. and Wilkinson S.G. (1988) Gram-positive bacteria. In Microbial Lipids (Vol. 1) (eds. Ratledge C. and Wilkinson S.G.) [M]. pp.117–202. Academic Press, London.
Pennanen T., Frostegard A., Fritze H., and Baath E. (1996) Phospholipid fatty acid composition and heavy metal tolerance of soil microbial communities along two heavy metal-polluted gradients in coniferous forests [J]. Applied and Environmental Microbiology. 62, 420–428.
Renella G., Mench M., van der Leliec D., Pietramellara G., Ascher J., Ceccherini M.T., Landi L., and Nannipieri P. (2004) Hydrolase activity, microbial biomass and community structure in long-term Cd-contaminated soils [J]. Soil Biology and Biochemistry. 36, 443–451.
Turpeinen R., Kairesalo T., and Haggblom M.M. (2004) Microbial community structure and activity in arsenic-, chromium-, and copper-contaminated soils [J]. FEMS Microbiology Ecology. 47, 39–50.
Wang Yuanpeng, Shi Jiyan, Wang Hui, Lin Qi, Chen Xincai, and Chen Yingxu (2007) The influence of soil heavy metals pollution on soil microbial biomass, enzyme activity, and community composition near a copper smelter [J]. Ecotoxicology and Environmental Safety. 67, 75–81.
Wendy M.W. and David A.W. (2007) The soil microbial community response when plants are subjected to water stress and defoliation disturbance [J]. Applied Soil Ecology. 37, 139–149.
Yao Huaiying, Xu Jianming, and Huang Changyong (2003) Substrate utilization pattern, biomass and activity of microbial communities in a sequence of heavy metal-polluted paddy soils [J]. Geoderma. 115, 139–148.
Yang Yuangen, Liu Congqiang, Xu Lei, Wu Pan, and Zhang Guoping (2004) Effects of heavy metal contamination on microbial biomass and community structure in soils [J]. Chinese Journal of Geochemistry. 23, 319–328.
Yang Yuangen, Paterson E., and Campbell C.D. (2001) Urban soil microbial features and their environmental significance as exemplified by Aberdeen City, UK [J]. Chinese Journal of Geochemistry. 20, 34–44.
Zhong Wenhui and Cai Zucong (2007) Long-term effects of inorganic fertilizers on microbial biomass and community functional diversity in a paddy soil derived from quaternary red clay [J]. Applied Soil Ecology. 36, 84–91.
Zogg G..P., Zak D.R., Ringleberg D.B., MaPbonald N.W., Pregitzer K.S., and White D.C. (1997) Compositional and functional shifts in microbial communities due to soil warming [J]. Soil Science Society of America Journal. 61, 475–481.
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Xie, X., Liao, M., Ma, A. et al. Effects of contamination of single and combined cadmium and mercury on the soil microbial community structural diversity and functional diversity. Chin. J. Geochem. 30, 366–374 (2011). https://doi.org/10.1007/s11631-011-0521-7
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DOI: https://doi.org/10.1007/s11631-011-0521-7