Summary
Brugmansia candida, an indigenous South American plant, produces the tropane alkaloids scopolamine and hyoscyamine, which are widely employed in medicine as anticholinergic agents. In this research, hairy roots of Brugmansia candida, obtained through infection with Agrobacterium rhizogenes LBA 9402, were employed to produce these tropane alkaloids in vitro. The effects of different concentrations of GA7 on kinetics of growth and alkaloid accumulation on two different hairy root clones of B. candida were analyzed, and the influence of GA7 on the number of new branches and rates of elongation was also studied. On clone 7A, GA7 at concentrations of 10−4, 10−1, and 1 mg/l increased the exponential growth rate. Levels of 10−1 and 10−4 mg/l GA7 elevated the scopolamine/hyoscyamine (S/H) ratios in the early phases of growth, but the sum of scopolamine plus hyoscyamine per flask (S + H) decreased during that period. When 1 mg/l GA7 was used, the highest S/H ratios were observed in late exponential/early stationary phases, but the highest S + H totals were obtained in mid-exponential phase. GA7 at levels of 10−1 and, especially, 1 mg/l exerted a positive effect on formation, emergence, and rate of elongation of lateral roots (clone 7A). On clone 7B, levels of 10−1 and 1 mg/l GA7 did not alter significantly the exponential growth rate. GA7 in concentrations of 10−1 mg/l induced increases in both S/H ratio and S + H totals in late phases of growth.
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Pitta-Alvarez, S.I., Giulietti, A.M. Effects of gibberellin GA7 on kinetics of growth and tropane alkaloid accumulation in hairy roots of Brugmansia candida . In Vitro Cell.Dev.Biol.-Plant 33, 147–153 (1997). https://doi.org/10.1007/s11627-997-0014-x
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DOI: https://doi.org/10.1007/s11627-997-0014-x