Summary
Rapid micropropagation of Saussurea lappa C. B. Clarke, an endangered medicinal plant endemic in the valley of Kashmir and western Himalayas of northern India, was achieved by culturing shoot tips (0.5–1 cm) of 2-wk-old seedlings on Murashige and Skoog’s medium (MS) supplemented with thidiazuron (TDZ, 0.45 µM). Although callus-free multiple shoots were obtained both on N6-benzyladenine- (BA) and TDZ-containing media, TDZ was most effective (90%) in inducing multiple shoots. Shoot tips containing proliferative buds were divided into equal halves and subcultured on MS liquid medium containing 0.225 µM TDZ for further multiplication and elongation. Multiplication of induced shoot buds was more effective when cultured in liquid medium than on agar-solidified medium. Shoots (8–10 cm) developed were rooted in MS medium containing naphthaleneacetic acid (NAA, 1.07 µM). Micropropagated plantlets were successfully transferred to soil after hardening.
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Johnson, T.S., Narayan, S.B. & Narayana, D.B.A. Rapid in vitro propagation of Saussurea lappa, an endangered medicinal plant, through multiple shoot cultures. In Vitro Cell.Dev.Biol.-Plant 33, 128–130 (1997). https://doi.org/10.1007/s11627-997-0010-1
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DOI: https://doi.org/10.1007/s11627-997-0010-1