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Adventitious shoot formation and plant regeneration from Pinus pinaster Sol. ex Aiton

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Summary

Pinus pinaster plants were regenerated from cotyledons excised from in vitro germinated seeds and axenically cultured on induction medium (GMD). 6-Benzyladenine (2.2 µM) induced the highest frequency of direct bud formation from cotyledons. An average of 13.1 ± 2.1 elongated shoots per cotyledon was obtained. Germination time influenced shoot induction, and the organogenic potential decreased with explant age. Cotyledons remained for 21 d on induction medium, and in order to promote adventitious shoot elongation, they were transferred to Gupta and Durzan’s DCR medium without growth regulators, containing 0.5% (wt/vol) activated charcoal and 3% (wt/vol) sucrose. Rooting was achieved by application of an indole-3-butyric acid, (396.6 µM) pulse (24 h at 4° C), followed by transfer to a sterile mixture of peat plus perlite (1:1 vol/vol). Ninety-eight to 100% of the regenerated plants were successfully acclimatized. All plants have survived after transfer to the field.

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  1. Centro de Biotecnologia Vegetal, Faculdade de Ciências de Lisboa, Edifício C2 Piso 1, Campo Grande, P-1700, Lisboa, Portugal

    Filomena Calixto & M. Salomé Pais

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  1. Filomena Calixto
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  2. M. Salomé Pais
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Calixto, F., Pais, M.S. Adventitious shoot formation and plant regeneration from Pinus pinaster Sol. ex Aiton. In Vitro Cell.Dev.Biol.-Plant 33, 119–124 (1997). https://doi.org/10.1007/s11627-997-0008-8

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  • DOI: https://doi.org/10.1007/s11627-997-0008-8

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