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Growth of in vitro–regenerated plants of Gerbera jamesonii following micropropagation in temporary immersion bioreactors

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Abstract

Gerbera represents one of the top five most important traditional cut flowers. A major factor influencing the success of the cut flower industry is vase life which in turn has a significant influence on consumer preference. Therefore, the present study considered the effect of silver nanoparticles (AgNPs) supplied in temporary immersion bioreactors (TIBs) on the growth and subsequent shelf life of gerbera flowers. The results showed an initial lag in the growth of plantlets produced from tissue culture, but this effect was reversed by the end of the study, particularly for plants exposed to AgNPs. Although AgNPs did not shorten the time to flowering (67 d in control plants compared with 73 d in AgNPs-treated plants), flower characteristics were not adversely affected by this delay. A significant finding from this study was the observation of improved vase life of flowers grown from plants treated with AgNPs (9.8 d) relative to control plants (8.2 d). This indicated the potential of AgNPs to sustain the longevity of cut flowers after harvest.

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Figure 1.
Figure 2.

Data availability

The data that support the findings of this study are available from the corresponding author (ME) upon reasonable request.

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Acknowledgements

This research was supported by the Bioplant Center (University of Ciego de Ávila Máximo Gómez Báez, Cuba); Universidad Autónoma Agraria Antonio Narro (México); Agricultural Research Council-Tropical and Subtropical Crops (South Africa); Centro de Nanociencias y Nanotecnología (Universidad Nacional Autónoma de México, Mexico); and Tomsk Polytechnic University (Russia). The authors are also grateful to Mr. Aldo Alarcon (CCS Cloroberto Echemendía, Ciego de Ávila, Cuba). The authors acknowledge the mutual grant No. 22-13-20032 of the Russian Science Foundation and Tomsk Region for improving the properties of Argovit™ silver nanoparticles.

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Authors and Affiliations

  1. Bioplant Center, University of Ciego de Ávila Máximo Gómez Báez, Ciego de Ávila, Cuba

    Osbel Mosqueda-Frómeta, Grisis M. Mosqueda-Rodríguez, Oscar Concepción, Maritza Escalona & José Carlos Lorenzo

  2. Universidad Autónoma Agraria Antonio Narro, Coahuila, México

    Barbarita Companioni

  3. Plant Improvement, Agricultural Research Council-Tropical and Subtropical Crops, Private Bag X11208, Nelspruit, 1200, South Africa

    Elliosha Hajari

  4. Centro de Nanociencias y Nanotecnología, Universidad Nacional Autónoma de México, Ensenada, Baja California, Mexico

    Nina Bogdanchikova

  5. Research School of Chemistry & Applied Biomedical Sciences, Tomsk Polytechnic University, Tomsk, 634050, Russia

    Alexey Pestryakov

Authors
  1. Osbel Mosqueda-Frómeta
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  2. Grisis M. Mosqueda-Rodríguez
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  3. Barbarita Companioni
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  4. Elliosha Hajari
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  5. Nina Bogdanchikova
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  6. Oscar Concepción
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  7. Maritza Escalona
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  8. Alexey Pestryakov
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  9. José Carlos Lorenzo
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Contributions

OMF, GMMR, BC, EH, NB, OC, ME, AP, and JCL designed the research; OMF and GMMR conducted the experiment; OMF, BC, EH, NB, OC, ME, AP, and JCL analyzed the data and wrote the paper; and ME and JCL had primary responsibility for the final content. All authors have read and approved the final manuscript.

Corresponding author

Correspondence to José Carlos Lorenzo.

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The authors declare no competing interests.

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Mosqueda-Frómeta, O., Mosqueda-Rodríguez, G.M., Companioni, B. et al. Growth of in vitro–regenerated plants of Gerbera jamesonii following micropropagation in temporary immersion bioreactors. In Vitro Cell.Dev.Biol.-Plant (2024). https://doi.org/10.1007/s11627-024-10429-w

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