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Standardization of in vitro multiplication technique for Areca concinna Thwaites, an endangered palm species for its conservation and utilization

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Abstract

Areca concinna Thwaites (Arecaceae) is an endangered plant species endemic to South-Western Sri Lanka and distributed primarily in Sri Lanka, India, and Southeast Asia. This palm was listed under endangered plants species by IUCN (The International Union for Conservation of Nature) due to habitat loss. Clonal propagation is essential for conservation and maintenance of this endangered species. In an effort to conserve this plant species, a protocol was developed for clonal multiplication of A. concinna Thwaites. Immature inflorescence, immature embryos, and mature embryos were tested for callogenesis and subsequent somatic embryogenesis in M72 basal medium supplemented with the auxins 2,4-D and picloram. Of the three explants, somatic embryos were obtained only from mature embryos. Callus multiplication and somatic embryo formation in M72 basal medium supplemented with 2,4-D were found to be better over picloram media. Serial transfer of explants from higher to lower concentrations was essential for sustaining the multiplication of callus as well as for induction of somatic embryos. Somatic embryos grown in Y3 or 1/2 Y3 basal medium supplemented with 1.0 mg L−1 BAP, 0.5 mg L−1 NAA, and 0.25 mg L−1 IBA exhibited shoot initiation and plantlet development. This protocol has its application in rapid multiplication of A. concinna Thwaites palms thereby enhancing the possibility of conservation of the endangered palm.

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References

  • Aliyu OM (2005) Application of tissue culture to cashew (Anacardium occidentale. p. L.) breeding: an appraisal. Afr J Biotechnol 4:1485–1489

    Google Scholar 

  • Al-Mayahi AMW, Ali AH, Shareef HJ (2018) Influence of cold pretreatment on shoot regeneration from callus in date palm (Phoenix dactylifera L.) cv. ‘Barhee.’ J Genet Eng Biotechnol 16:607–612

    Article  PubMed  PubMed Central  Google Scholar 

  • Aparna V, Neema M, Chandran KP, Muralikrishna KS, Karun A (2023) Enhancement of callogenesis from plumular explants of coconut (Cocos nucifera) via exogenous supplementation of amino acids and casein hydrolysate. Curr Hort 11:40–43

    Article  Google Scholar 

  • Campos-Boza S, Vinas M, Solórzano-Cascante P, Holst A, Steinmacher DA, Guerra MP, Jiménez VM (2022) Somatic embryogenesis and plant regeneration from transverse thin cell layers of adult peach palm (Bactris gasipaes) lateral offshoots. Front Plant Sci 13:995307

    Article  PubMed  PubMed Central  Google Scholar 

  • Carsono O, Juwendah E, Liberty SS, Damayanti F, Rachmadi M (2021) Optimize 2, 4-D concentration and callus induction time enhance callus proliferation and plant regeneration of three rice genotypes. Biodiversitas 22:2555–2560

    Article  Google Scholar 

  • Chan JL, Saénz L, Talavera C, Hornung R, Robert M, Oropeza C (1998) Regeneration of coconut (Cocos nucifera L.) from plumule explants through somatic embryogenesis. Plant Cell Rep 17:515–521

    Article  CAS  PubMed  Google Scholar 

  • Cueto CA, Ebert AW, Rillo EP, Orense OD (1996) In vitro regeneration of coconut (Cocos nucifera L.) from immature inflorescences of tall cultivars. In III Int Symp in Vitro Cult Hortic Breed 447:121–122

    Google Scholar 

  • Eeuwens C, Blake J (1977) Culture of coconut and date palm tissue with a view to vegetative propagation. Acta Hortic 78:277–286

    Article  Google Scholar 

  • EL-Gioushy SF, Liu R, Fan HK (2020) A complete protocol to reduce browning during coconut (Cocos nucifera L.) tissue culture through shoot tips and inflorescence explants. Plant Arch 20:2196–2220

    Google Scholar 

  • Fernando SC, Vidhanaarachchi VRM, Weerakoon LK, Santha ES (2010) What makes clonal propagation of coconut difficult? Asia-Pac J Mol Biol Biotechnol 18:163–165

    Google Scholar 

  • Goh DKS, Michaux FN, Monteuuis O, Bon MC (1999) Evidence of somatic embryogenesis from root tip explants of rattan Calamus manan. In Vitro Cell Dev Biol - Plant 35:424–427

  • Huong LTL, Baicco M, Hug BP, Burno M, Santilachi R, Rosati P (1999) Somatic embryogenesis of Canary Island datepalm. Plant Cell Tiss Org Cult 56:1–7

    Article  Google Scholar 

  • Johnson D (1998) Areca concinna, The IUCN Red List of Threatened Species 1998:e.T38186A10099202

  • Karun A, Siril EA, Radha E, Parthasarathy A (2004) Somatic embryogenesis and plantlet regeneration from leaf and inflorescence explants of arecanut (Areca catechu L.). Curr Sci 86:1623–1628

    Google Scholar 

  • Karunaratne S, Periyapperuma K (1989) Culture of immaure embryos of coconut (Cocos nucifera L.): callus proliferation and somatic embryogenesis. Plant Sci 62:247–253

    Article  Google Scholar 

  • Laukkanen H, Rautiainen L, Taulavuori E, Hohtola A (2000) Changes in cellular structures and enzymatic activities during browning of Scots pine callus derived from mature buds. Tree Physiol 20:467–475

    Article  CAS  PubMed  Google Scholar 

  • Mahalakshmi A, Khurana JP, Khurana P (2003) Rapid induction of somatic embryogenesis by 2, 4-D in leaf base cultures of wheat (Triticum aestivum L.). Plant Biotechnol 20:267–273

    Article  CAS  Google Scholar 

  • Mendiburu FD (2020) Agricolae: statistical procedures for agricultural research. R package version 1.3–3. https://CRAN.R-project.org/package=agricolae. Accessed 15 Jul 2022

  • Murthy KN, Pillai RNS (1982) Botany, In: Bavappa KVA, Nair MK, Kumar PT (Eds.), The arecanut palm, Central Plantation Crops research Institute, Kasaragod. P 11–49

  • Neema M, Aparna V, Chandran KP (2022) Contrast analysis recommends flame sterilization for surface depuration in coconut (Cocos nucifera L.) meristem culture. Curr Hort 10:41–44

    Article  Google Scholar 

  • Panaia M, Senaratna T, Bunn E, Dixon KW, Sivasithamparam K (2000) Micropropagation of the critically endangered Western Australian species, Symonanthus bancroftii (F Muell.) L. Haegi (Solanaceae). Plant Cell Tiss Org Cult 63:23–29

    Article  CAS  Google Scholar 

  • Parsad R (2005) Transformation of data. In: Parsad R, Srivastava R, Gupta VK (eds) Design and analysis of agricultural experiments. Indian Agricultural Statistics Research Institute, New Delhi, p 637−647

  • Prathibha VH, Hegde V, Sharadraj KM, Nidhina K, Nagaraja NR, Chaithra M (2015) Identification of sources of resistance against Phytophthora in arecanut. In: Book of Abstracts - 3rd International Symposium on Phytophthora, Bengaluru, India, 9–12th September 2015, p 40

  • R Core Team (2022) R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. https://www.R-project.org/. Accessed 18 Jul 2022

  • Raju CR (2006) Direct in vitro shoot development from immature rachilla explants of coconut (Cocos nucifera L.). Cord 22:51–58

    Google Scholar 

  • Sáenz L, Chan JL, Narvaez M, Oropeza C (2018) Protocol for the micropropagation of coconut from plumule explants. In: Loyola-Vargas VM, Ochoa-Alejo N (eds) Plant cell culture protocols. Humana Press, New York, NY, USA, pp 161–170

    Chapter  Google Scholar 

  • Sandoval-Cancino G, Saenz L, Chan JL, Oropeza C (2016) Improved formation of embryogenic callus from coconut immature inflorescence explants. In Vitro Cell Dev Biol - Plant 52:367–378

    Article  CAS  Google Scholar 

  • Shareefa M, Thomas RJ, Sreelekshmi JS, Rajesh MK, Karun A (2019) In vitro regeneration of coconut plantlets from immature inflorescence. Curr Sci 117:813–820

    Article  CAS  Google Scholar 

  • Tabiyeh DT, Bernard F, Shacker H (2006) Investigation of glutathione, salicylic acid, and GA3 effects on browning in Pistacia vera shoot tips culture. Acta Hortic 726:201

    Article  CAS  Google Scholar 

  • The Plant List (2013) Version 1.1. Published on the internet http://www.theplantlist.org/ (accessed 3rd December 2018)

  • Verdeil JL, Huet C, Grosdemange F, Buffard-Morel J (1994) Plant regeneration from cultured immature inflorescences of coconut (Cocos nucifera L.) evidence for somatic embryogenesis. Plant Cell Rep 13:218–221

    CAS  PubMed  Google Scholar 

  • Vidhanaarachchi VRM, Weerakoon LK (1997) Callus induction and direct shoot formation in in vitro cultured immature inflorescence tissues of coconut. Cocos 12:39–43

    Article  Google Scholar 

  • Zayed EMM (2017) Direct organogenesis and indirect somatic embryogenesis by in vitro reversion of mature female floral buds to a vegetative state. In: Al-Khayri, JM, Jain, SM, Johnson DV (Eds.) Date palm biotechnology protocols volume I: tissue culture applications, methods in molecular biology. Humana Press, New York, p. 47-59

  • Zheng MY, Konzak CF (1999) Effect of 2, 4-D on callus induction and plant regeneration in anther culture of wheat. Plant Cell Rep 19:69–73

    Article  CAS  PubMed  Google Scholar 

Download references

Funding

The authors acknowledge ICAR, India for providing the necessary funds to carry out the research.

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Authors and Affiliations

  1. ICAR-Central Plantation Crops Research Institute, Kasaragod, Kerala, India

    Aparna Veluru, Sandip Shil & Sudha Raju

  2. ICAR-Indian Institute of Oil Palm Research, Research Centre Palode, Palode, Kerala, India

    Neema Mohamed

  3. ICAR-Indian Agricultural Research Institute, Jharkhand, Gauria Karma, Hazaribagh, India

    Krishna Prakash

  4. Department of Food and Agriculture, UAEU University, Al Ain, UAE

    K. Kavya

  5. National Institute of Plant Genomic Research, New Delhi, India

    S. Anand

Authors
  1. Aparna Veluru
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  2. Neema Mohamed
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  3. Sandip Shil
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  4. Krishna Prakash
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  5. K. Kavya
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  6. S. Anand
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  7. Sudha Raju
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Correspondence to Neema Mohamed.

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Veluru, A., Mohamed, N., Shil, S. et al. Standardization of in vitro multiplication technique for Areca concinna Thwaites, an endangered palm species for its conservation and utilization. In Vitro Cell.Dev.Biol.-Plant (2024). https://doi.org/10.1007/s11627-024-10425-0

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