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Alternative system for micropropagation of Prunus campanulata (Maxim.)

  • Plant Tissue Culture
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Abstract

Herein, an in vitro multiplication protocol for Prunus campanulata was evaluated. The effects of 15.0, 30.0, and 45.0 g L−1 sucrose and three flask sealing methods (PVC film, rigid polypropylene lid, and rigid lid with membrane) were analyzed at the multiplication, rooting, and acclimatization stages. For in vitro multiplication, shoot tips of approximately 1 cm in length containing two pairs of leaves from seedlings germinated in vitro in Woody Plant Medium (WPM) were used. Evaluations considered the multiplication of shoot apexes in three subcultures (at 40, 80, and 120 d) for survival, number of shoots, shoot length, number of leaves, and multiplication rate. In the rooting phase, the rooting percentage, number of roots, and root length were assessed. In the acclimatization phase under shaded and full sun conditions, seedling survival and biometric characteristics were evaluated, including stem diameter, height, and number of leaves. The addition of sucrose to the medium, at a concentration of 45.0 g L−1, associated with sealing using PVC plastic film, showed inadequate results for in vitro multiplication of P. campanulata. The addition of sucrose to the culture medium in concentrations ranging from 15.0 to 30.0 g L−1 favored in vitro rooting. The use of a permeable membrane for sealing enabled the growth of more vigorous shoots in terms of percentage of rooting and root length, and these seedlings stood out in the acclimatization phases with greater rates of survival and improved biometric characteristics.

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Acknowledgements

The authors thank the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – Brasil (CAPES) – Finance Code 001. We thank Isabel Homczinski, Marina Romaniuk, Anália Surkamp, Thainah Candido, and Odimeia Teixeira for support in the laboratory and analysis. We also thank Evelyn Roberta Nimmo for the language review of the manuscript.

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Authors and Affiliations

  1. Programa de Pós-Graduação em Ciências Florestais, Department of Forestry Engineering, UNICENTRO, Universidade Estadual do Centro-Oeste, Professora Maria Roza Zanon de Almeida, Irati, Paraná, 84505-677, Brazil

    Daniela Sanson, Alexandre Techy de Almeida Garrett, Isy Cavalhães Rodrigues, Gabriel de Magalhães Miranda, Fabricio William de Ávila, Rogério Bobrowski & Fabiana Schmidt Bandeira Peres

  2. Department of Agricultural Sciences, UNIMONTES, Universidade Estadual de Montes Claros, Janaúba, Minas Gerais, 39448-524, Brazil

    Cristiane Alves Fogaça

  3. Department of Forest Science, UFSM, Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul, 97105-900, Brazil

    Ezequiel Gasparin

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  1. Daniela Sanson
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  2. Alexandre Techy de Almeida Garrett
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  3. Isy Cavalhães Rodrigues
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  4. Gabriel de Magalhães Miranda
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Correspondence to Daniela Sanson.

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Sanson, D., de Almeida Garrett, A.T., Rodrigues, I.C. et al. Alternative system for micropropagation of Prunus campanulata (Maxim.). In Vitro Cell.Dev.Biol.-Plant (2024). https://doi.org/10.1007/s11627-024-10424-1

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