Abstract
In this study, adventitious organogenesis from mature tissues of ‘Verna 51’ and ‘Fino 49’ lemon cultivars has been studied, as well as their relation with endogenous cytokinins and ethylene production. Thus, nodal explants in which buds were completely removed, obtained from in vitro shoot cultures of both lemon cultivars, were cultured in regeneration media, and the regeneration rate and the explants that regenerated were recorded. Also, after regeneration, endogenous cytokinin content was analyzed, as well as the ethylene emitted by the explants of both cultivars. After 8 wk in the culture medium, the explants of ‘Fino 49’ were more regenerative than those of ‘Verna 51’. Likewise, the endogenous content of cytokinins was globally higher in ‘Verna 51’, even if the levels of isopentenyladenine and isopentenyladenosine were higher in ‘Fino 49’. Additionally, ‘Verna 51’ explants had higher concentrations of zeatin than ‘Fino 49’, and the same content of N6-benzyladenine and N6-benzyladenosine. In like manner, the ethylene level emitted by the explants was higher in ‘Verna 51’. According to these results, the amount of endogenous cytokinins as well as the ethylene production by the explant plays an important role in the organogenesis of mature explants of C. limon.
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The authors want to thank Fernando Córdoba for his technical assistance in the laboratory.
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This work was supported by the European Regional Development Fund.
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Pérez-Jiménez, M., Celdrán-Sánchez, V. & Pérez-Tornero, O. Role of endogenous cytokinins and ethylene in adventitious shoot regeneration in lemon (Citrus limon). In Vitro Cell.Dev.Biol.-Plant 58, 787–793 (2022). https://doi.org/10.1007/s11627-022-10286-5
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DOI: https://doi.org/10.1007/s11627-022-10286-5