Abstract
LED (light-emitting diode) light is an economical and effective light source for inducing plant growth and development. The impact of different LED lights was investigated on callus growth, antioxidant enzymes, oxidative damage, and secondary metabolite production of H. reticulatus. The in vitro callus initiated from hypocotyl were subcultured on Murashige and Skoog (MS) medium and transferred to shelves with different LED lights (white, red, blue, and red-blue) and darkness for 3 wk. Results showed darkness and red-blue light were the best for maximum biomass, protein content, and cell viability. Red and blue LED lights motivated cells to be round in shape and non-viable, and induced more lipoxygenase activity, lipid peroxidation, and hydrogen peroxide contents as compared to darkness and red-blue light. Different LED lights enhanced total sugar content as compared to darkness. The maximum activities of superoxide dismutase (SOD) and catalase (CAT) were observed in calluses treated to red-blue light and darkness, while peroxidase (POX) activity was more induced under blue and red lights. LED light also enhanced the accumulation of total phenol, flavonoid, alkaloid, secondary metabolites, and DPPH scavenging activity, especially in red-blue light. This research may establish new strategies by red-blue LED light for sustainable production of secondary metabolite and biomass in H. reticulatus callus.
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The author is grateful to Mr. Esmailian for designing the LED light selves.
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The author received financial support from the Aerospace Research Institute.
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Hassanpour, H. Potential impact of red-blue LED light on callus growth, cell viability, and secondary metabolism of Hyoscyamus reticulatus. In Vitro Cell.Dev.Biol.-Plant 58, 256–265 (2022). https://doi.org/10.1007/s11627-021-10232-x
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DOI: https://doi.org/10.1007/s11627-021-10232-x