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Soybean androgenesis II: non-gametophytic morphologies in isolated microspore culture

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Abstract

Gametophytic embryogenesis is a process capable of accelerating trait discovery and cultivar development in plant-breeding programs. However, recalcitrance to morphogenic stimuli has limited such work in many crops of interest, especially among fabaceous species such as soybean (Glycine max [L.] Merrill). In complementary studies, donor plant and culture conditions were documented to successfully divert isolated soybean microspores from a gametophytic pathway; these findings were reinforced through transcriptomic analysis of microspores subjected to pretreatment temperature stress, suggesting cellular dedifferentiation through suppression of the pollen developmental program. In the present study, cytological characteristics were observed in isolated microspore culture, which evidenced non-gametophytic development. These phenotypes included (i) inhibition of daughter cell specialization, (ii) pollen dimorphism, and (iii) exine rupture, among others. The outlined morphologies were placed into the context of established gametophytic embryogenesis platforms, further supporting previous claims of cell fate redirection in soybean microspores. The present study provides insight into gametophytic embryogenesis induction via isolated microspore culture and may prove useful for the investigation of soybean androgenesis.

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Acknowledgments

The authors acknowledge and are appreciative of the intellectual contribution from the Corteva Agriscience Doubled Haploid and Core Technologies teams, especially Dr. Sreekala Chellamma, Dr. Pon Samuel, Dr. Yue Yun, and Pa Lor. Additional technical support was provided by Dr. Jianfeng Xu at Arkansas State University.

Funding

This research was supported by grants from USDA-NIFA Non-Land Grant Colleges of Agriculture Capacity Building award number 2018-70001-28762, Arkansas Soybean Promotion Board, University of Arkansas System Division of Agriculture, and Corteva Agriscience Open Innovation.

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Authors and Affiliations

  1. Molecular Biosciences Graduate Program, Arkansas State University, State University, Jonesboro, AR, 72467-0837, USA

    Brett Hale

  2. Arkansas Biosciences Institute, Arkansas State University, State University, Jonesboro, AR, 72467-0639, USA

    Brett Hale, Callie Phipps, Naina Rao, Caroline Kelley & Gregory C. Phillips

  3. College of Agriculture, Arkansas State University, State University, Jonesboro, AR, 72467-1080, USA

    Gregory C. Phillips

  4. University of Arkansas System Division of Agriculture, Agricultural Experiment Station, State University, Jonesboro, AR, 72467-2340, USA

    Gregory C. Phillips

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Correspondence to Brett Hale.

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Editor: Nancy Reichert

The original version of this article was revised: Panels c, e, and f of Fig. 2 were incorrect in the article as originally published and have been replaced. In addition, the information provided for panel c in the figure caption has been modified.

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Hale, B., Phipps, C., Rao, N. et al. Soybean androgenesis II: non-gametophytic morphologies in isolated microspore culture. In Vitro Cell.Dev.Biol.-Plant 57, 356–364 (2021). https://doi.org/10.1007/s11627-020-10144-2

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