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Achieving highly efficient rooting procedure in three Pyrus communis cultivars by response surface methodology

  • Plant Tissue Culture
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Abstract

Pyrus communis is a difficult-to-root species, and its optimal rooting depends on applying an accurate concentration of various chemicals. Since statistical modeling can be applied to estimate the approximate range of factors needed for the success of in vitro plant propagation, we tried to optimize the rooting medium using response surface methodology. Therefore, sucrose and polyvinyl alcohol were selected as independent variables in the central composite design of response surface methodology with α = 1. Based on the pre-test, it was found that the independent variables for the root formation were 2 to 3% sucrose and 0.5 to 1.5 g L−1 polyvinyl alcohol in studied cultivars. The statistical analysis results showed that the quadratic models were significant, with high R2 values > 0.9 for rooting rate response. The optimized values for independent variables in rooting of the studied cultivars included 2% sucrose, 1.5 g L−1 polyvinyl alcohol in ‘Williams’; 2.85% sucrose, 1.47 g L−1 polyvinyl alcohol in ‘Natanzi’; and 3% sucrose, 0.99 g L−1 polyvinyl alcohol in ‘Ghosi’. A separate validation experiment detected that the experimental values of the studied responses are in close agreement with predicted values in all cultivars. For the first time, the methodology was developed using a central composite design to optimize the rooting in P. communis.

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Acknowledgments

We expressed appreciation to Ferdowsi University of Mashhad for the financial support of this research (cod: 31803) and for providing the experimental site and laboratory facilities.

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Authors and Affiliations

  1. Department of Horticultural Science and Landscape, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

    Atefe Ameri, Gholam Hossein Davarynejad & Ali Tehranifar

  2. Department of Biotechnology and Plant Breeding, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

    Nasrin Moshtaghi

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  1. Atefe Ameri
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  2. Gholam Hossein Davarynejad
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  3. Nasrin Moshtaghi
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  4. Ali Tehranifar
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Correspondence to Gholam Hossein Davarynejad.

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The authors declare that they have no conflict of interest.

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Editor: Randall Niedz

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Ameri, A., Davarynejad, G.H., Moshtaghi, N. et al. Achieving highly efficient rooting procedure in three Pyrus communis cultivars by response surface methodology. In Vitro Cell.Dev.Biol.-Plant 57, 398–408 (2021). https://doi.org/10.1007/s11627-020-10107-7

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  • DOI: https://doi.org/10.1007/s11627-020-10107-7

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