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Variation of phenotype, ploidy level, and organogenic potential of in vitro regenerated polyploids of Pyrus communis

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Abstract

A wide range of phenotypic variation was observed among neopolyploids obtained from the diploid pear cultivar ‘Fertility’ by in vitro colchicine treatment. The variant plantlets had alterations in leaf characteristics. Neopolyploids had significantly different ratios of leaf length to leaf width compared to the diploid control. Shoot regeneration from leaf explants and rooting ability from in vitro shoots of neopolyploids was examined. Regeneration frequencies of shoots from leaf explants of seven of the nine neopolyploids were significantly decreased compared to the diploid control. The organogenic potential of neopolyploids was highly genotype-dependent for both shoots and roots. Tetraploid clone 4x − 4 failed to regenerate shoots from leaf explants and the pentaploid clone 5x − 2 failed to root from in vitro shoots. The results suggest that polyploidization caused the decrease in or loss of in vitro organogenic potential. Regenerated shoots derived from neopolyploids showed different phenotypes, depending on the ploidy of the donor plant.

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Acknowledgments

This study was supported by Shandong Natural Science Foundation (Y2008D50), Shandong Outstanding Scientist Research Award Project (01DS48) and Shandong Science and Technology Development Plan poject (2008GG2HZ09018).

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Authors and Affiliations

  1. Shandong Institute of Pomology, Taian, 27100, Shandong, China

    Qingrong Sun, Hongyan Sun, Huifeng Li & Li Xin

  2. Appalachian Fruit Research Station, USDA, ARS, Kearneysville, WV, 25430-2771, USA

    Richard L. Bell

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  1. Qingrong Sun
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  2. Hongyan Sun
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  3. Richard L. Bell
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  4. Huifeng Li
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  5. Li Xin
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Correspondence to Qingrong Sun.

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Sun, Q., Sun, H., Bell, R.L. et al. Variation of phenotype, ploidy level, and organogenic potential of in vitro regenerated polyploids of Pyrus communis . Plant Cell Tiss Organ Cult 107, 131–140 (2011). https://doi.org/10.1007/s11240-011-9965-z

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  • DOI: https://doi.org/10.1007/s11240-011-9965-z

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